Trajectory analysis of cell populations from the regenerating intestinal tissue.
(A) UMAP plot of all identified clusters. (B) RNA velocity embedded in UMAP of all main clusters. (C) UMAP of re-clustering of cells from C0, C1, C3, C4, C5, and C9. (D) RNA velocity analysis results from the subset from panel (C). (E) Jitter plot of Slingshot pseudotime of cells from C0, C1, C3, C4, C5 and C9. Pseudotime resulted in two lineages one containing C5 and the other C 9. (F) UMAP of re-clustered anlage cells corresponding to C0, C1, C3, and C4(G) RNA velocity results from the cluster results from panel F. (H) UMAP of panel F plot overlayed with pseudotime results of Slingshot. Color represent pseudotime values from 0 (blue) to 60 (yellow). (I) Jitter plot showing the Slingshot pseudotiume of cells from each cell cluster.