Peer review process
Not revised: This Reviewed Preprint includes the authors’ original preprint (without revision), an eLife assessment, public reviews, and a provisional response from the authors.
Read more about eLife’s peer review process.Editors
- Reviewing EditorIvan VelascoUniversidad Nacional Autónoma de México, Mexico City, Mexico
- Senior EditorSofia AraújoUniversity of Barcelona, Barcelona, Spain
Reviewer #1 (Public review):
Summary:
The paper describes the initial characterization of Eml3 knockout mice. Eml3 global inactivation leads to delayed embryonic development, perinatal lethality apparently due to failure to inflate lungs, and a cobblestone brain-like phenotype represented by focal neuronal ectopias in the marginal zone or subarachnoid space of dorsal telencephalon. The neural ectopias are associated with interruptions in the pial basal membrane (PBM), which appear around E11.5. The authors also confirmed previously described protein interactions, using coIP-MS experiments of placenta and embryonic tissues (TUBB3, several 14-3-3 proteins, and DYNLL). The authors generated mice carrying a TQT86AAA homozygous mutation in EML3 (a motif required for EML3-DYNLL interactions) that were normal and showed no focal neuronal ectopias, indicating that this particular protein interaction is dispensable. The authors propose Eml3 knockout mice as a model of cobblestone brain malformation.
Strengths:
The brain phenotype described in this work is relevant for the neural development field and with potential clinical relevance. The initial phenotyping is appropriate but will require additional experiments to establish the cause of the failure to inflate the lungs. The study shows convincing data regarding the main characteristics of the brain phenotype and data supporting the timing when these abnormalities arise during development.
Weaknesses:
The study would benefit from clearer evidence and additional experiments that would help to establish the molecular and cellular mechanisms underlying the brain phenotype, the central topic of the work.
Reviewer #2 (Public review):
Summary:
In this manuscript, the authors investigate the role of the microtubule-binding protein EML3 during cortical development through the generation and characterization of an Eml3 mouse mutant. The authors focus mainly on the effects of EML3 loss on brain development, although Eml3 mouse mutants also present with developmental delay and growth restriction, and die perinatally due to respiratory distress caused by delayed maturation of the lungs. The main finding in the developing cortex is the presence of focal neuronal ectopias, which contain neurons from all cortical layers, as revealed by immunostaining. The authors use electron microscopy to show that ectopias seem to be caused by disruption to the pial basement membrane at early stages of development, which allows neurons to breach through it. To find a functional link between EML3 and the observed phenotype, studies are conducted that demonstrate expression of EML3 in radial glia cells and mesenchymal cells, both cell types involved in the formation and maintenance of the pial basement membrane. Furthermore, interaction partners for EML3 are identified through coIP-MS analysis, including tubulin beta-3, 14-3-3 proteins, and cytoplasmic dynein light chain. However, mice carrying a mutant EML3 allele engineered to abolish the interaction between EML3 and cytoplasmic dynein light chain do not recapitulate any of the symptoms of complete EML3 loss.
Strengths:
The manuscript offers several important strengths that contribute significantly to the field. This study presents the first characterization of Eml3 knockout animals, providing novel insights into the role of Eml3 in vivo. Information on Eml3 function so far was restricted to cell culture data, so the results in this manuscript start to fill an important gap in our knowledge about this microtubule-binding protein. The experimental approach is carefully designed, with appropriate controls that ensure the reliability of the data. Moreover, the authors have addressed a key challenge in the analysis, namely the developmental delay of the knockout animals. By implementing a strategy to match developmental stages between wild-type and knockout groups, they allow for meaningful and valid comparisons between the two genotypes. Importantly, the authors have successfully generated three different Eml3 mutant mouse lines (knockout, floxed, and with disrupted binding to cytoplasmic dynein light chain), which are very valuable tools for the broader scientific community to further study the roles of this gene in development and disease in the future.
Weaknesses:
While the manuscript presents valuable data, there are also several weaknesses that limit the overall impact of the study. Most notably, there is no clear mechanistic link established between the loss of Eml3 function and the observed phenotype, leaving the biological significance of the findings somewhat speculative, as it is not straightforward how a microtubule-associated protein can have an impact on the stability of the pial basement membrane. In this respect, but also in general for the whole manuscript, there seems to be a considerable amount of experimental work that has been conducted but is not presented, possibly due to the negative nature of the results. At least some of those results could be shown, particularly (but not only) the stainings for the composition of the ECM components. Additionally, the phenotype reported appears to be dependent on the genetic background, as it is absent in the CD1 strain. This observation raises concerns as to how robust the results are and how much they can be generalized to other mouse strains, but, more importantly, to humans. There is no data included in the manuscript about the generation and analysis of the Eml3AAA/AAA mouse line. This is an important omission, especially as no details on the validation or phenotypic characterization of this additional mouse line are provided. Including these elements would greatly strengthen the rigor and interpretability of the work, especially if that mouse line is to be shared with the scientific community.
Reviewer #3 (Public review):
Summary:
This work aims to understand the role of Echinoderm Microtubule-associated Protein-like 3 (EML3) in embryogenesis and neocortical development. Importantly, this work shows that depletion of EML3 causes focal neuronal ectopias by disrupting the structural integrity of the pial basement membrane, describing a new model of cobblestone brain malformation. Another member of the EML family, EML1, has already been shown to trigger neuronal migration disorders, particularly subcortical band heterotopia, by affecting cell polarity. The results presented here point to a different mechanism of action. The authors show that EML3 is expressed in radial glia cells and mesenchymal cells in the pial region, and upon EML3 depletion (i.e., Eml3 mutant mice), the pial basement membrane is structurally damaged, allowing migrating neuroblasts to ectopically migrate through. Answering, in this case, that the weakening of the pial basement membrane is a prerequisite for focal neuronal ectopias. The authors provide a meticulous characterization of the Eml3 mutant mice, strengthening the conclusions of the results.
Strengths:
The authors provide a very detailed analysis of the defects observed in Eml3 mutant mice, by providing not only results by inferred day of conception but also by classifying embryos by their number of somite pairs.
Weaknesses:
(1) Besides the data provided in the figures, the authors report a significant amount of experiments/results as "Data not shown". Negative data is still important data to report, and the authors may want to choose some crucial "not shown data" to report in the manuscript.
(2) Results in Figure 3A apparently contradict results in 3B. A better explanation of the results should improve understanding of the data. Even though the conclusion that the "onset and progression of neurogenesis is normal in Eml3 null mice" seems logical based on the data, the final numbers are not (Figure 3A) and this should be acknowledged, as well.
(3) The authors should define which cell types are identified by SOX1 and PAX6.