Introduction

The light-sensitive outer segment organelle of vertebrate photoreceptor cells is a modified cilium containing a stack of disc-shaped membranes, or “discs”. While initially formed as serial evaginations of the photoreceptor plasma membrane, mature discs are separated from this membrane and are fully enclosed inside the outer segment (Steinberg et al., 1980; Burgoyne et al., 2015; Ding et al., 2015; Volland et al., 2015) (reviewed in (Spencer et al., 2020)). Membrane enclosure involves the formation of highly curved, hairpin-shaped disc rims. Two homologous tetraspanin proteins, PRPH2 (also known as peripherin-2 or rds) and ROM1, localize specifically to disc rims (Connell and Molday, 1990; Travis et al., 1991; Bascom et al., 1992) (reviewed in (Stuck et al., 2016)). Within the disc rim, PRPH2 and ROM1 form homo- and heteromeric complexes (Bascom et al., 1992; Goldberg et al., 1995; Goldberg and Molday, 1996b; Goldberg and Molday, 1996a) that are assembled into larger oligomers connected by disulfide bonds (Goldberg et al., 1998; Loewen and Molday, 2000; Chakraborty et al., 2009; Zulliger et al., 2018). Three parallel interconnected chains of these oligomers are wrapped around the circumference of the mature disc and support its hairpin-shaped structure (Pöge et al., 2021).

Despite a high level of structural homology between PRPH2 and ROM1 (Li et al., 2003), the consequences of their mutations for photoreceptor health are quite different. Around 200 mutations of PRPH2 are shown to cause a heterogeneous set of inherited retinal diseases, including retinitis pigmentosa, cone-rod dystrophy and macular dystrophies (Landrum et al., 2018; Peeters et al., 2021). Yet, mutations in ROM1 typically cause digenic retinitis pigmentosa in conjunction with mutations in PRPH2 (Kajiwara et al., 1994; Dryja et al., 1997), except for a handful of reports of mutations in ROM1 in patients without accompanying PRPH2 mutations (Bascom et al., 1995; Sakuma et al., 1995; Reig et al., 2000). With regard to animal models, the Prph2 knockout mouse, commonly known as the rds mouse, has a complete failure of outer segment formation (Cohen, 1983; Jansen and Sanyal, 1984), while the Rom1 knockout mouse forms outer segments with relatively minor structural abnormalities (Clarke et al., 2000). This discrepancy could be explained, at least in part, by the importance of the cytoplasmic C-terminus of PRPH2, which has been shown to retain membranes at the photoreceptor cilium, thereby allowing their remodeling into outer segment discs (Salinas et al., 2017). However, most of the disease-associated PRPH2 mutations spare its C-terminus, so the complete explanation of this discrepancy awaits further investigation of the role of ROM1.

In this study, we investigated the role of ROM1 in the formation of photoreceptor disc rims and the process of disc enclosure. We first revisited the phenotype of the Rom1 knockout (Rom1^-/-) mouse and made several novel observations. Using quantitative proteomics, we showed that the knockout of ROM1 causes a compensatory increase in the relative disc content of PRPH2. Despite the total tetraspanin content being essentially the same as in WT discs, Rom1^-/- discs displayed a delay in their maturation and an increase in outer segment diameter. In addition, Rom1^-/- discs lacked the indentations of their rims, known as incisures, present in WT discs. These changes could be explained, at least in part, by an alteration in tetraspanin oligomerization likely arising from a rearrangement of intramolecular disulfide bridge(s) in PRPH2. Strikingly, these morphological phenotypes can be rescued by transgenic overexpression of PRPH2, suggesting that ROM1 is not absolutely necessary for the formation of normal outer segments. In another set of experiments, we investigated whether disc rims could be formed in a knockin mouse in which the tetraspanin core of PRPH2 was replaced with that of ROM1. While the knockin photoreceptors failed to form orderly disc stacks, outer segment membranes preserved the ability to form hairpin-shaped rims. Together, these results demonstrate that, despite its contribution to disc formation and intrinsic ability to support disc rim structure, ROM1 can be replaced by an excess of PRPH2 for the formation of a normal outer segment.

Results

Loss of ROM1 causes a compensatory increase in the relative outer segment content of PRPH2

Three proteins, rhodopsin, PRPH2 and ROM1, comprise ∼98% of the total transmembrane protein material in normal discs (Skiba et al., 2023). Recently, it has been proposed that the overall dimensions of a disc are determined by the molar ratio between the total tetraspanin content (PRPH2 and ROM1) forming the disc rim and rhodopsin forming the disc surface (Lewis et al., 2023). Therefore, interpreting the phenotype of the Rom1^-/- mouse requires an understanding of how the loss of ROM1 affects the outer segment content of PRPH2. This is particularly important because discs deficient in PRPH2 display a compensatory increase in the relative amount of ROM1 (Lewis et al., 2023), suggesting that the opposite (i.e., a compensatory increase in the amount of PRPH2 when there is a deficiency in ROM1) may also be true.

To address this possibility, we employed a quantitative mass spectrometry approach (Skiba et al., 2023) recently used to determine the molar ratio among PRPH2, ROM1 and rhodopsin in other mutant photoreceptors (Lewis et al., 2023). In these experiments, the outer segment content of PRPH2 was determined as a molar fraction of rhodopsin. Because the packing density of rhodopsin in discs is unaffected by any changes in disc dimensions (Liang et al., 2004), this parameter reflects the relative content of PRPH2 in each disc, independent of any changes in outer segment length or volume. We found that the relative content of PRPH2 in Rom1^-/- outer segments increased to an ∼1:12 molar ratio to rhodopsin from an ∼1:18 ratio in WT outer segments (Table 1; see raw data in Supplementary Table 1). Strikingly, this 1:12 ratio is equal to the ratio between total tetraspanin (PRPH2 + ROM1) and rhodopsin in WT outer segments. Therefore, the knockout of ROM1 leads to a compensatory increase in the relative disc content of PRPH2.

Table 1.

Loss of ROM1 delays disc enclosure causing increased outer segment diameter and occasional disc overgrowth

The retinal phenotype of Rom1^-/- mice was analyzed at postnatal day 30 (P30), as photoreceptor cell degeneration was just beginning at this age (Figs. 1A,B). Interestingly, light microscopy imaging was sufficient to identify that the outer segment layer of these mice appeared disorganized and even shortened (Figs. 1A,C). Please note that this and the three subsequent figures also include data obtained with a mouse overexpressing PRPH2 on the Rom1^-/-background (PRPH2 OE/Rom1^-/-), which will be described below, to facilitate a side-by-side comparison of all three phenotypes. Using transmission electron microscopy (TEM), we observed a number of outer segment structural abnormalities (Fig. 2 and Supplementary Fig. 1), consistent with those noted in the original report (Clarke et al., 2000). In general, outer segments appeared to be shorter and wider, with some of them displaying overgrown disc membranes not aligned in a stack (yellow arrows, Fig 2B). The degree of this overgrowth varied, with the extended membranes occasionally wrapping around the entire outer segment.

Figure 1.

Figure 2.

Because these ultrastructural defects are reminiscent of those from several PRPH2 mutant lines in which photoreceptor disc enclosure is affected (Lewis et al., 2021), we assessed the status of disc enclosure in Rom1^-/-outer segments. We contrasted retinal tissue with tannic acid and uranyl acetate instead of the traditionally used osmium tetroxide (Ding et al., 2015). This technique yields a darker staining of newly forming, “open” discs exposed to the extracellular space than discs fully enclosed within the outer segment. This approach revealed that even outer segments lacking gross abnormalities in Rom1^-/- mice have an increased number of open discs at their base (Figs. 3A,B). Whereas WT rods contained a relatively constant number of ∼7 open discs (consistent with previous reports (Ding et al., 2015; Volland et al., 2015; Lewis et al., 2021)), Rom1^-/- rods had an average of ∼14 open discs. In addition, the number of open discs in Rom1^-/- rods was much more variable than in WT rods, with up to ∼30 open discs occasionally observed. Lastly, we quantified outer segment diameters of Rom1^-/-rods and found that they were on average ∼35% wider than WT rods (Fig. 3C), which is close to the ∼43% increase previously reported in (Clarke et al., 2000).

Figure 3.

These findings show that ROM1 contributes to the process of disc enclosure and that the compensatory increase in the disc content of PRPH2 does not fully replace ROM1 in this capacity. Accordingly, the increase in outer segment diameter may be explained by prolonged delivery of disc membrane material to each disc before it becomes fully enclosed. On occasion, an expansion of open discs becomes uncontrolled leading to formation of membranous whorls.

Another phenotype of Rom1^-/- mice was revealed using TEM of tangentially sectioned retinas (Fig. 4). Normal mouse discs contain a single deep indentation of their rims, called an incisure (yellow arrowheads, Fig. 4). In contrast, no incisures were observed in Rom1^-/- discs. Because the rims of both disc circumference and incisure are built from PRPH2/ROM1, the lack of incisures in Rom1^-/- discs may be explained by the entire pool of remaining tetraspanin being deposited at the circumference of larger discs.

Figure 4.

ROM1 regulates the supramolecular organization of PRPH2

We next sought to address why the compensatory increase in the disc content of PRPH2 in Rom1^-/-rods is insufficient to support normal disc morphogenesis. In this context, it is important to consider that defects in PRPH2 oligomerization, without an accompanying decrease in PRPH2 levels, also lead to defects in disc enclosure (Lewis et al., 2021). Therefore, we reasoned that loss of ROM1 may modulate the status of PRPH2 oligomerization. In fact, the idea that ROM1 may regulate the formation of high order PRPH2 oligomers has been put forward in a previous study (Loewen and Molday, 2000).

To address the possibility that PRPH2 oligomerization may be disrupted without ROM1, we first performed SDS-PAGE of retinal lysates from WT and Rom1^-/-mice under non-reducing conditions (i.e., no DTT in samples). Under these conditions, PRPH2 is represented by two bands, one corresponding to ∼35 kDa monomers and another to ∼75 kDa dimers linked by a disulfide bond (Fig. 5A). Both bands are somewhat diffuse, likely reflecting the heterogeneous status of PRPH2 glycosylation. The majority of PRPH2 in WT retinas is represented by dimers, consistent with its origin from large, disulfide-bound oligomers.

Figure 5.

Two differences were observed in Rom1^-/- retinas. First, there was an ∼50% increase in the fraction of PRPH2 that runs in a monomeric state (Figs. 5A,B), indicating that PRPH2 oligomerization may be negatively affected by loss of ROM1. Second, the monomeric form of PRPH2 was represented by a doublet, consistent with a previous observation (Stuck et al., 2015). This doublet was consolidated into a single band under reducing conditions in the presence of DTT (Fig. 5C), which suggests that the two bands in Rom1^-/- retinas represent pools of PRPH2 molecules with different patterns of internal disulfide bonds. Apart from the C150 residue involved in intermolecular disulfide bonding of a PRPH2 molecule to another PRPH2 or ROM1, there are six other conserved cysteines in PRPH2 that are involved in intramolecular disulfide bonding (Goldberg et al., 1998). Therefore, it is possible that ROM1 contributes to PRPH2 assuming the intermolecular disulfide bond conformation optimal for tetraspanin oligomerization at the disc rim.

To further investigate the status of PRPH2 oligomerization in Rom1^-/- mice, we utilized a technique typically used to describe PRPH2 and ROM1 complexes of various sizes. In this approach, complexes are extracted from disc membranes in the presence of an non-ionic detergent and separated by velocity sedimentation on a sucrose gradient under non-reducing conditions (Clarke et al., 2000; Goldberg et al., 2001; Ding et al., 2004; Chakraborty et al., 2008; Chakraborty et al., 2009; Stuck et al., 2014; Zulliger et al., 2018; Milstein et al., 2020; Lewis et al., 2021). We performed velocity sedimentation of lysates from both WT and Rom1^-/-retinas followed by SDS-PAGE of gradient fractions under non-reducing conditions (Figs. 5D,E). The distribution of the PRPH2 monomeric form across fractions was unaffected by the ROM1 knockout. In both WT and Rom1^-/- retinas, it was most abundant in fractions #6-8, shown to correspond to PRPH2 core complexes (Chakraborty et al., 2008). However, the loss of ROM1 shifted the distribution of the disulfide-bound PRPH2 dimers to smaller oligomeric forms, consistent with a previous report (Conley et al., 2019). Taken together, these data show that loss of ROM1 does indeed affect the status of PRPH2 oligomerization and suggest that abnormal PRPH2 oligomerization underlies the defects in disc enclosure in Rom1^-/- mice.

Transgenic overexpression of PRPH2 can compensate for the loss of ROM1

We next investigated whether a further increase in the level of PRPH2 could improve the morphological defects observed in Rom1^-/-outer segments. We employed a transgenic line that expresses PRPH2 in both rods and cones under control of the human IRBP promoter (Nour et al., 2004). While originally called NMP, we refer to this line as PRPH2 OE (PRPH2 overexpressor). In the current study, we used PRPH2 OE mice with a single copy of this transgene, which has been shown to express ∼30% excess PRPH2 over WT levels (Nour et al., 2004). PRPH2 OE mice were crossed with Rom1^-/- mice and retinas were analyzed as for WT and Rom1^-/- mice (Figs. 1-3). Strikingly, the gross morphological defects observed in Rom1^-/- mice were significantly improved by transgenic overexpression of PRPH2 (Fig. 2A). The outer segment length was somewhat restored (Figs. 1A,C), although the degree of this effect did not reach statistical significance. Furthermore, PRPH2 overexpression rescued the disc enclosure defect of Rom1^-/-outer segments by restoring the number of open discs to the WT level (Figs. 3A,B). In addition, PRPH2 OE/Rom1^-/- outer segments had normal diameters (Figs. 3A,C) and their discs contained incisures (Fig. 4). These data indicate that, whereas ROM1 contributes to disc formation in WT rods, it can be functionally replaced by a sufficient excess of PRPH2.

ROM1 is able to form disc rims in the absence of PRPH2

While we have shown that ROM1 contributes to the formation of disc rims, it is unclear whether it can do so in the absence of PRPH2. Because disc formation is completely abolished in the absence of PRPH2, we could not address this question using PRPH2 knockout mice. Instead, we utilized a knockin mouse model, called RRCT (Conley et al., 2019), in which the tetraspanin core of PRPH2 was replaced with that of ROM1 (Fig. 6A). This chimeric protein contains the C-terminus of PRPH2 required for retaining disc membranes at the outer segment base (Salinas et al., 2017), while replacing the tetraspanin core required for its oligomerization and functioning in disc rim formation (Salinas et al., 2017; Conley et al., 2019).

Figure 6.

As previously reported (Conley et al., 2019), homozygous RRCT photoreceptors formed rudimentary outer segments (Fig. 6B). The small number and size of these outer segment-like structures is likely explained by a very low expression of the chimeric protein (Conley et al., 2019), which may lead to a low efficiency of disc membrane retention. We now show that some of these structures contain stacked disc membranes that are partially enclosed within the outer segment plasma membrane. Importantly, these disc membranes have discernible rims with a characteristic hairpin-like shape. This finding indicates that ROM1 alone is, in principle, able to support the formation of disc rims in the absence of PRPH2.

In an additional set of experiments, we explored the oligomerization status of ROM1 and the RRCT chimera in the absence of PRPH2. Unfortunately, the severe disruptions of outer segments in homozygous RRCT mice precluded us from obtaining a sufficient amount of retinal lysate to perform this analysis. Instead, we used an in vitro system in which we co-transfected equal amounts of RRCT and ROM1 in COS7 cells (Fig. 6C). In these cells, RRCT and ROM1 co-localized in structures that were distinct from the endoplasmic reticulum, as evident from the lack of co-localization with the endoplasmic reticulum marker, calreticulin, suggesting that they were normally processed in the biosynthetic membranes. Velocity sedimentation of lysates from these cells revealed that both RRCT and ROM1 sediment in fractions #6-8 (Figs. 6D-E), which is the same as for PRPH2/ROM1 core complexes from WT photoreceptors. These data indicate that heterologously expressed RRCT and ROM1 can form core tetraspanin complexes, even in the absence of PRPH2, that are able to support disc rim formation.

Disc rims can be formed without disulfide bonds between tetraspanin molecules

In a final experiment, we investigated whether the formation of intermolecular disulfide bonds between tetraspanins is dispensable for disc rim formation. We have previously shown that disulfide bonds between PRPH2 molecules are not absolutely required for this function because disc rims were formed in Prph2^C150S/C150S knockin mice lacking the cysteine residue forming this bond (Lewis et al., 2020). Yet ROM1 also forms intermolecular disulfide bonds (Chakraborty et al., 2008), which raises the question of whether disc rim formation in Prph2^C150S/C150S knockin mice was driven by disulfide-linked ROM1 molecules.

To address this question, we analyzed disc rim structure in Rom1^-/-mice bearing the Prph2^C150S/C150S mutation. Outer segments of these mice had morphological defects comparable to those of Prph2^C150S/C150S mice (Fig. 7A). Nonetheless, orderly disc stacks were occasionally observed in these retinas. Higher magnification images of these stacks revealed the presence of disc rims which looked comparable to those in Prph2^C150S/C150S retinas (Fig. 7B). This finding demonstrates that, whereas disulfide bonds connecting tetraspanin molecules are essential for maintaining the overall outer segment structure, these bonds are not absolutely required for the formation of disc rims.

Figure 7.

In addition, staining with tannic acid/uranyl acetate revealed that the outer segments of Prph2^C150S/C150S/Rom1^-/- mice contained both open (yellow arrows, Fig. 7A) and enclosed discs (yellow arrowheads, Fig. 7A). This indicates that disulfide bonds among PRPH2 and ROM1 molecules are also dispensable for disc enclosure.

Discussion

In this study, we analyzed the role of ROM1 in supporting the formation of outer segment discs. In addition to the previously described morphological defects of Rom1^-/-outer segments, we showed that these outer segments experience a delay in disc enclosure. This delay leads to an increase in disc diameter, loss of incisures and occasional uncontrolled disc membrane outgrowth. Remarkably, these defects can be reversed by sufficient overexpression of PRPH2, indicating a functional redundancy between these two tetraspanin proteins.

The idea of redundancy between PRPH2 and ROM1 is supported by the finding that mutations in both genetically interact to modulate disease (Kajiwara et al., 1994; Dryja et al., 1997). While individual PRPH2 mutations have greater phenotypic consequences, this could relate to both the unique role of the C-terminal tail of PRPH2 in the initial stages of disc formation (Salinas et al., 2017) as well as the fact that there is twice as much PRPH2 as ROM1 in outer segments, at least in mice (Kedzierski et al., 1999; Loewen and Molday, 2000; Skiba et al., 2023).

While evidence that ROM1 mutations alone may cause human visual pathology is limited (Bascom et al., 1995; Sakuma et al., 1995; Reig et al., 2000), photoreceptors of Rom1^-/- mice eventually degenerate (Clarke et al., 2000). We propose that the delay in disc enclosure is the primary defect underlying this degeneration. Because discs remain open longer than normal, they may incorporate more than a normal amount of membranous material, which is continuously delivered to the outer segment. This can lead to disc membrane overgrowth and, eventually, the formation of highly dysmorphic membrane whorls. This progressive collapse of outer segment ultrastructure would eventually lead to cell death. Notably, this same progression of structural defects occurs in certain mice in which PRPH2 oligomerization is affected (Lewis et al., 2021).

But why is the phenotype of the heterozygous rds mouse containing ∼50% of normal PRPH2 protein (Hawkins et al., 1985; Cheng et al., 1997) much more severe than the Rom1^-/- mouse lacking 100% of ROM1? This difference is not intuitive because the molar ratio between PRPH2 and ROM1 is ∼2:1 and, therefore, each mouse is expected to have ∼2/3 of the normal tetraspanin content. However, there is a compensatory increase in outer segment tetraspanin in each of these two mice. We now show that relative content of total tetraspanin in Rom1^-/- discs is essentially the same as in WT discs (∼1:12 molar ratio to rhodopsin) due to a compensatory increase in PRPH2. On the other hand, there is only a partial compensatory increase of ROM1 in heterozygous rds mice (∼1:15 molar ratio of PRPH2+ROM1 to rhodopsin) (Lewis et al., 2023). The mechanism behind these increases remains to be elucidated, but regardless, the lesser severity of the Rom1^-/- phenotype could be explained, at least in part, by a larger degree of this compensatory tetraspanin increase.

Yet, the complete restoration of the total tetraspanin content in Rom1^-/- outer segments does not fully rescue their morphological phenotype. This implies that, in normal photoreceptors, ROM1 contributes some unique functional features to the properties of tetraspanin oligomers. In fact, our data suggest that the loss of ROM1 alters both the intra- and intermolecular properties of the remaining PRPH2. We found that ROM1 likely facilitates an optimal internal disulfide bond arrangement of PRPH2 in addition to promoting the formation of larger oligomeric chains of PRPH2. One possible explanation for the ability of ROM1 to potentially facilitate the optimal disulfide bond arrangement of PRPH2 may relate to the intracellular trafficking of these two proteins. Whereas the majority of PRPH2 is normally trafficked through the unconventional secretory pathway from the endoplasmic reticulum that bypasses the Golgi (Tian et al., 2014; Zulliger et al., 2015; Pearring et al., 2021), ROM1 was shown to direct a small fraction of PRPH2 through the conventional secretory pathway (Conley et al., 2019). It is intriguing to speculate that a shift in the intramolecular disulfide bonds of PRPH2 in the absence of ROM1 may arise from a complete loss of PRPH2 trafficking through the conventional pathway. Nonetheless, the outer segment deficiencies arising from loss of ROM1 were nearly completely overcome by a sufficient excess of PRPH2.

Of particular interest is our finding that discs of Rom1^-/-mice lack incisures. A recent study put forth a model in which the total length of the disc rim, including the incisure, is determined by the molar ratio between the total tetraspanin protein and rhodopsin (Lewis et al., 2023). The lack of incisures in Rom1^-/- discs may not appear intuitive since the total tetraspanin content in these discs is essentially the same as in WT discs. However, Rom1^-/- discs also have an increased diameter, which allows the total rim length of WT and Rom1^-/- discs to be about the same. The reason why such a tradeoff between the disc diameter and the presence/absence of an incisure exists remains to be determined, although it is likely to relate to the kinetics of disc enclosure.

In conclusion, our study shows that ROM1 makes distinct contributions to the formation of disc rims and the process of disc enclosure. Nonetheless, a sufficient excess of PRPH2 is able to compensate for the loss of ROM1, indicating that ROM1 is redundant to PRPH2 as a molecular building block of photoreceptor disc rims. Given that excess PRPH2 can rescue defects associated with loss of ROM1, it is exciting to speculate that PRPH2 overexpression can prevent the long-term photoreceptor degeneration associated with either loss of ROM1 or deficiencies arising from mutations in PRPH2 itself. It also remains to be seen whether the opposite is also true, whereby an excess ROM1 may rescue certain defects associated with mutations in PRPH2.

Materials and Methods

Mouse (Mus musculus) husbandry

Animal maintenance and experiments were approved by the local Institutional Animal Care and Use Committee (IACUC; University of Houston, TX, USA) and guidelines as stated by the Association for Research in Vision and Ophthalmology (Rockville, MD). The generation of the Prph2^C150Smouse was previously described in (Stuck et al., 2014). The generation of the RRCT mouse was previously described in (Conley et al., 2019). The generation of the PRPH2 OE mouse was previously described in (Nour et al., 2004). The Rom1^-/- mouse, described in (Clarke et al., 2000), was generously provided by Roderick R McInnes (McGill University). All mice were genotyped to ensure that they did not contain either the rd8 (Mattapallil et al., 2012) or rd1 (Pittler et al., 1993) mutations commonly found in inbred mouse strains. All mice were on a C57BL/6 genetic background bearing the RPE65 L450 variant and housed under a 12/12 hour diurnal light (∼30 lux) cycle.

Transmission electron microscopy (TEM)

For all main figures, fixation and processing of mouse eyes for TEM was performed as described previously (Ding et al., 2015). In the afternoon, anesthetized mice were transcardially perfused with 2% paraformaldehyde, 2% glutaraldehyde and 0.05% calcium chloride in 50 mM MOPS (pH 7.4) resulting in exsanguination. Enucleated eyes were fixed for an additional 2 h in the same fixation solution at room temperature. Eyecups were dissected from fixed eyes, embedded in 2.5% low-melt agarose (Precisionary, Greenville, NC) and cut into 200 µm thick slices on a Vibratome (VT1200S; Leica, Buffalo Grove, IL). Agarose sections were stained with 1% tannic acid (Electron Microscopy Sciences, Hatfield, PA) and 1% uranyl acetate (Electron Microscopy Sciences), gradually dehydrated with ethanol and infiltrated and embedded in Spurr’s resin (Electron Microscopy Sciences). 70 nm sections were cut, placed on copper grids and counterstained with 2% uranyl acetate and 3.5% lead citrate (19314; Ted Pella, Redding, CA). The samples were imaged on a JEM-1400 electron microscope (JEOL, Peabody, MA) at 60 kV with a digital camera (BioSprint; AMT, Woburn, MA). Image analysis and processing was performed with ImageJ. For Supplementary Figure 1, fixation, processing, and imaging of mouse eyes for TEM was performed as described previously (Stricker et al., 2005).

Light microscopy of histological sections

Plastic embedded blocks generated for TEM were sectioned through the optic nerve in 500 nm sections and stained with methylene blue for light microscopy as previously described (Lobanova et al., 2008). Images were taken with a confocal microscope (Eclipse 90i and A1 confocal scanner; Nikon) with a 60× objective (1.4 NA Plan Apochromat VC; Nikon) using Nikon NIS-Elements software. Image analysis and processing was performed with ImageJ.

Preparation of retinal lysates

Eyecups were dissected and immediately frozen using liquid nitrogen and stored at −80°C prior to processing. Lysates were prepared essentially as previously described (Stuck et al., 2014). In short, individual eyecups were lysed in 200 µl solubilization buffer (PBS, pH 7.0, containing 1% Triton X-100, 5 mM EDTA, 5 mg/ml NEM and protease inhibitors (Roche, Mannheim, Germany)). Samples were incubated on ice for 1 h prior to being centrifuged for 30 min at 20,000g at 4°C. The supernatant was collected and subjected to either Western blot analysis directly or velocity sedimentation.

Western blotting of retinal lysates

Protein concentration was assayed by using a colorimetric Bradford assay (Bio-Rad, Hercules, CA, USA). Western blotting was performed essentially as previously described (Spencer et al., 2016). In short, lysates were incubated with Laemmli sample buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol and 1% Bromophenol Blue) with or without 100 mM DTT for reducing or non-reducing blots respectively. Samples containing 10 µg of total protein were incubated at 90°C for 5 minutes and run on a 10-20% Tris-HCl gel. Gels were transferred onto PVDF membrane and blocked with Intercept (PBS) Blocking Buffer (Li-Cor, Lincoln, Nebraska) with 0.25% Tween-20. Blots were incubated overnight at 4°C with 1:1000 dilution of polyclonal rabbit anti-PRPH2 C-terminal antibody (Kedzierski et al., 1999). After primary antibody incubation, blots were washed and incubated with 1:10000 dilution of donkey anti-rabbit DyLight 800 (Invitrogen, Carlsbad, CA) for 2 h. All experiments were repeated at least three times. Blots were imaged using Odyssey CLx imaging system (Li-Cor).

Velocity sedimentation using sucrose gradients

Sucrose gradients were prepared as previously described (Chakraborty et al., 2009). In short, gradients of 5–20% sucrose were prepared by sequentially layering 0.5 ml each of 20, 15, 10 and 5% sucrose solutions in PBS with 0.1% Triton X-100 and 10 mM NEM and allowing them to sit at room temperature for 1 h to equilibrate. Gradients were then chilled on ice for 30 minutes prior to loading lysate and centrifugation at 40,000 rpm on a TLS-55 swinging bucket rotor (Beckman Coulter, Brea, CA) for 16 hours at 4°C. The bottom of each tube was pierced with a 21G needle and 12 fractions of ∼180 µl each were collected. Western blotting was performed as described above using 15 µl samples for each fraction.

Cell culture experiments

COS7 cells were transfected with 7.5 µg of RRCT-FLAG and ROM1 mammalian expression constructs that were previously described (Conley et al., 2010; Conley et al., 2019) using a calcium phosphate transfection protocol as previously described (Conley et al., 2019). After 48 hours, cells were processed for immunofluorescent staining or velocity sedimentation as described below. Experiments were repeated three independent times.

Immunofluorescent staining was performed as described in (Conley et al., 2010). After blocking, coverslips were incubated overnight in primary antibodies to label RRCT, ROM1 and ER membranes as follows: 1) 1:2 dilution of monoclonal mouse anti-RRCT (2E7; described in (Zulliger et al., 2015), 2) 1:400 dilution of polyclonal rabbit anti-ROM1 (2Rom1; described in (Ding et al., 2005)), and 3) 1:250 dilution of polyclonal chicken anti-calreticulin at 1:250 (ab2908; Abcam, Cambridge, UK). After washing, cells were incubated with 1:000 dilution of donkey anti-mouse Alexa 568 (Invitrogen), donkey anti-rabbit Alexa 647 (Invitrogen) and goat anti-chicken Alexa 488 (Invitrogen) secondary antibodies for 1 h. Cells were mounted in ProlongGold with DAPI (ThermoFisher). Images were captured on a BX-62 spinning disk confocal microscope equipped with an ORCA-ER camera (Olympus, Japan) and analyzed with Slidebook™ 5.2 software (Intelligent Imaging Innovations, Denver, CO). Images from the spinning disk confocal microscope were deconvolved using the nearest neighbors paradigm. Images were captured with 100x/1.40 oil objectives, and exposure times and display settings (brightness and contrast) for all images were normalized to a control section where primary antibody was omitted during processing. No gamma adjustments were made to immunofluorescent images.

For velocity sedimentation, scraped cells were lysed in the same solubilization buffer used to prepare retinal extracts. Lysates were separated as described above (200 µg protein lysate/gradient). Resulting gradient fractions were separated by SDS-PAGE under reducing conditions and blots were probed with either 1:500 dilution of monoclonal mouse anti-RRCT (2B7; described in (Conley et al., 2014)) or monoclonal mouse anti-ROM1 (2H5, described in (Conley et al., 2014)). Blots were probed with goat anti-mouse and anti-rabbit HRP secondaries (SeraCare, Milford, MA), imaged on a Bio-Rad ChemiDoc imager and analyzed in Image Lab Software version 6.0.1 (Bio-Rad).

Quantitative mass spectrometry

A crude preparation of rod outer segments was obtained as described in (Lewis et al., 2023). Dissected mouse retinas were vortexed in 8% OptiPrep in mouse Ringer’s solution (containing 130 mM NaCl, 3.6 mM KCl, 2.4 mM MgCl₂, 1.2 mM CaCl₂, and 10 mM HEPES, pH 7.4) that was adjusted to 314 mOsm. The preparation was briefly left on ice to allow the remaining retinal tissue to settle. The supernatant was removed and centrifuged at 20,000 x g. Pelleted outer segments were gently washed with mouse Ringer’s solution before lysis with 2% SDS in PBS. Protein concentration was measured with the Bio-Rad Protein Assay kit (Bio-Rad). Samples containing 5-10 µg of protein were mixed with 0.25-0.5 µg BSA (used as an internal standard in this analysis) and cleaved with 1 µg trypsin/LysC mix (Promega, Madison, WI) using the SP3 beads protocol described in (Hughes et al., 2014). The combined digest of outer segments and BSA was mixed with the digest of a chimeric protein consisting of concatenated tryptic peptides of outer segment proteins, including rhodopsin, PRPH2 and ROM1, which is described in (Skiba et al., 2023). Mass spectrometry, data processing and data analysis were also performed as described in (Skiba et al., 2023). For each genotype, a total of two biological replicates were analyzed.

Experimental design and statistical analysis

For the quantification of the number of photoreceptor nuclei (Fig. 1B), photoreceptor nuclei were counted in 100 µm boxes at 500 µm intervals from the optic nerve spanning 2000 µm in each direction for three mice of each genotype, as previously described (Lobanova et al., 2018). Two-way ANOVA was performed to determine statistical significance across genotype and location.

For the quantification of the number of open discs (Fig. 3B), darkly-stained new discs at the base of the rod outer segment were counted until the first lightly-stained enclosed disc. 3 retinas were collected of each genotype with the number of outer segments analyzed as follows: WT1, 44; WT2, 40; WT3, 68; Rom1^-/-1, 87; Rom1^-/-2, 42; Rom1^-/-3, 44; PRPH2 OE/Rom1^-/-1, 48; PRPH2 OE/Rom1^-/-2, 35; PRPH2 OE/Rom1^-/-3, 59. Data were plotted with samples separated, while statistical analysis was performed on the averages within each retina (n=3 for each genotype). One-way ANOVA with Tukey’s multiple comparisons test was performed to determine statistical significance across genotypes. For the quantification of outer segment diameter (Fig. 3C), 3 retinas were collected of each genotype with the number of outer segments analyzed as follows: WT1, 88; WT2, 97; WT3, 92; Rom1^-/-1, 83; Rom1^-/-2, 81; Rom1^-/-3, 85; PRPH2 OE/Rom1^-/-1, 75; PRPH2 OE/Rom1^-/-2, 66; PRPH2 OE/Rom1^-/-3, 78. Data were plotted with samples separated, while statistical analysis was performed on the averages within each retina (n=3 for each genotype). One-way ANOVA with Tukey’s multiple comparisons test was performed to determine statistical significance across genotypes.

Densitometric analysis of non-saturated bands from Western blots (Figs. 5,6) was performed using Image Lab software v4.1 (Bio-Rad) and ImageJ. For quantification of the PRPH2 monomer:dimer ratio (Fig. 5B), 3 samples were used for both WT and Rom1^-/- mice. Unpaired t-test was performed to determine statistical significance of the PRPH2 monomer:dimer ratio between WT and Rom1^-/- retinas). For the velocity sedimentation analysis of PRPH2 supramolecular organization (Fig. 5E), 11 samples were used for WT and 4 samples were used for Rom1^-/- mice. Two-way ANOVA was performed to determine statistical significance across genotypes and fractions followed by Sidak’s multiple comparisons post-hoc test to determine statistical significance between genotypes in each fraction for the disulfide-bound form. For the quantification of ROM1 and RRCT in each fraction of transfected COS7 cells (Fig. 6E), 3 samples were analyzed.

All experiments were performed with mice of randomized sex. Sample sizes were determined based on previous published experiments. Data were graphed with Prism 9 (GraphPad, San Diego, CA) with error bars depicting the S.E.M. Statistical analyses were performed using Prism 9 (GraphPad). Where noted, statistical values are depicted in graphs with asterisks as follows: p < 0.05, *; p < 0.01, **, p < 0.001, ***; p < 0.0001, ****.

Acknowledgements

This work was supported by the National Institutes of Health grants EY030451 (VYA), EY005722 (VYA), EY010609 (MIN and MRA), EY034671 (MIN), EY033763 (TRL), AG070915 (SMC) and an Unrestricted Award from Research to Prevent Blindness Inc. (Duke University).

Competing interests

No competing interests declared.

Supplementary Material

Supplementary Figure 1.

Supplementary Table 1. Quantification of molar ratios between tetraspanins and rhodopsin in WT and Rom1^-/- mice – raw data