SPAG7-deficiency causes obesity and insulin resistance
A. Graphical representation of an ENU-driven forward genetic screen.
B. Images of WT and SPAG7 KO littermates at 32 weeks of age.
C. WT vs SPAG7 KO body weight over time. n = 7. Significance was assessed by Welch’s two sample t-test.
D. WT vs SPAG7 KO lean mass over time. n = 7. Significance was assessed by Welch’s two sample t-test.
E. WT vs SPAG7 KO fat mass over time. n = 7. Significance was assessed by Welch’s two sample t-test.
F. Body length measured from nose to base-of-tail. n = 7. Significance was assessed by Welch’s two sample t-test.
G. Bone mineral density, as determined by DEXA scan. n = 7. Significance was assessed by Welch’s two sample t-test.
H. Heart weight. n = 7. Significance was assessed by Welch’s two sample t-test.
I. Gastrocnemius muscle weight. n = 7. Significance was assessed by Welch’s two sample t-test.
J. Tibialis anterior muscle weight. n = 7. Significance was assessed by Welch’s two sample t-test.
K. Kidney weight. n = 7. Significance was assessed by Welch’s two sample t-test.
L. Brain weight. n = 7. Significance was assessed by Welch’s two sample t-test.
M. Liver weight. n = 7.
N. Plasma triglyceride levels following 8-hour fast. n = 5. Significance was assessed by Welch’s two sample t-test.
O. Plasma triglyceride levels following 8-hour fast with a 2-hour ad-lib refeed. n = 5. Significance was assessed by Welch’s two sample t-test.
P. Plasma total cholesterol levels following 8-hour fast. n = 5. Significance was assessed by Welch’s two sample t-test.
Q. Plasma total cholesterol levels following 8-hour fast with a 2-hour refeed. n = 5.
R. Plasma NEFA levels following 8-hour fast. n = 5. Significance was assessed by Welch’s two sample t-test.
S. Plasma NEFA levels following 8-hour fast with a 2-hour refeed. n = 5. Significance was assessed by Welch’s two sample t-test.
T. Blood glucose levels following an oral glucose challenge. n = 7. Significance was assessed by Welch’s two sample t-test.
U. Plasma insulin levels following an oral glucose challenge. n = 7. Significance was assessed by Welch’s two sample t-test.
V. Blood glucose levels following an IP insulin challenge. n = 7. Significance was assessed by Welch’s two sample t-test. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.