Investigation of neurons activated by social behavior with Hr38 FISH.
(A) Hr38 expression in the neurons labeled with P1a-split GAL4, Tk-GAL4, and aSP2-split GAL4 drivers. magenta: myr::GFP, yellow: Hr38 HCR signals. Dotted lines depicted the outlines of myr::GFP labeled cell bodies. Arrows: cell bodies of Hr38 positive neurons. (B) - (D) Expression level of Hr38 HCR signals (top) and percentage of Hr38 positive neurons (bottom) in P1a (B), Tk (C), and aSP2 (D) after different behavioral episodes. Aggression: aggressive behavior against another male, Dead: interaction with a dead male, No Food: interaction with another male without food (no fighting), Mesh: interaction with another male, separated with mesh (no physical contact), Courtship: courtship behavior with a virgin female. Bottom: They are grouped in categories according to the characters (a, b, c). In each group, the behavioral conditions do not have a statistically significant difference (p < 0.05, chi-square test). Number of neurons analyzed; P1a-Control: 135, P1a-Aggression: 84, P1a-Dead Male: 126, P1a-No Food: 115, P1a-Mesh: 87, P1a-Courtship: 114, Tk-Control: 45, Tk-Aggression: 69, Tk-Dead Male: 61, Tk-No Food: 63, Tk-Mesh: 82, Tk-Courtship: 66, aSP2-Control: 20, aSP2-Aggression: 36, aSP2-Dead Male: 23, aSP2-No Food: 49, aSP2-Mesh: 36, aSP2-Courtship: 24.