(A) Formaldehyde cross-linked chromatin was subjected to MNase digestion with increasing amounts on microccocal nuclease (from 1 to 5 units). De-crosslinked DNAs were separated on a 3% agarose gel and ∼60 bp and ∼120 bp mono- and di-nucleosomes were observed. Markers (M) indicate *50 bp and **150 bp. (B) The counts of AA, AT, TA, TT or CC, CG, GC, GG dinucleotides are reported at each position showing an enrichment of G/C nucleotides and a depletion of A/T nucleotides at the dyad relative to the end points of the protected fragment. This differs from the observation of Bailey et al. (2000), where GC, AA and TA dinucleotides were repeated at ∼10-bp intervals in recombinant archaeal histone B from Methanothermus fervidus (rHMfB) (Bailey et al., 2000). (C) The sequence logo of a nucleosome-binding site in Hfx. volcanii centered at the nucleosome midpoint. There is a significant GC enrichment towards the nucleosome midpoint. This is exhibited using both bit score and probability measures.