Chromatin is an ancient innovation conserved between Archaea and Eukarya
- University of Toronto, Canada
Figures
Figure 1
Micrococcal nuclease digestion produces nucleosomal fragments from crosslinked Hfx. volcanii chromatin.
(A) Formaldehyde cross-linked chromatin was subjected to MNase digestion with increasing amounts on microccocal nuclease (from 1 to 5 units). De-crosslinked DNAs were separated on a 3% agarose gel …
Figure 2
Nucleosome occupancy in Haloferax volcanii.
(A) Degree of normalized nucleosome occupancy in aggregate for the main chromosome. As observed in eukaryotes, there is a prominent nucleosome-depleted region (NDR) at the transcriptional start site …
Figure 3
Nucleosome-depleted regions at the 5′ and 3′ ends of transcripts.
As observed in eukaryotes, NDRs are also found at the transcriptional termination sites in Hfx. volcanii. Both 5′ and 3′ end profiles are overlaid in this figure for comparison. The 5′ NDR is, on …
Figure 4
Chromatin architecture is conserved at the 5′ end of transcripts across eukaryotes and archaea.
Due to the smaller size of archaeal nucleosome DNA, the occupancy has a shorter periodicity. Figure adapted with permission from Chang et al. (2012).
Figure 5
Sample screenshot of all data tracks loaded into the Savant genome browser (Fiume et al., 2010).
The nucleosome sequence data is displayed, and the periodicity reflects protected and unprotected fragments after MNase digestion (magnitude of peak is not considered). Peaks represent nucleosome …
Additional files
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Supplementary file 1
A table describing the 32 novel transcripts identified in Hfx. volcanii.
- https://doi.org/10.7554/eLife.00078.008
