qRT-PCR was used to quantitate d1EGFP mRNA after transfection of various constructs (Syngr3 [synaptogyrin 3], Glrb [Glycine receptor b], Dlg3 and Syt2 [Synaptotagmin 2]). Quantitation of steady state changes in RNA levels (YAAY relative to YCAY constructs) were 50%, 25%, 65%, 48%, respectively, for Syngr3, Glrb, Dlg3 and Syt2. The Y-axis represents the relative mRNA levels (YCAY/YAAY). Interestingly, for Dlg3, a small 3′ UTR construct (115 nt) did not show any change, while a longer construct (214 nt) did, even though both harbored the YCAY element, suggesting that additional elements or RNA structures may be important for binding of NOVA to this 3′ UTR YCAY element. Asterisks indicate p<0.05.