Sugar is an endogenous cue for juvenile-to-adult phase transition in plants
- Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, China
- Graduate School of Chinese Academy of Sciences, China
- Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, China
- East China Normal University, China
- Kunming Institute of Botany, China
Figures
Figure 1
Phenotypic analyses of the mir156a mir156c double mutant.
(A) MIR156A and MIR156C genomic regions. Arrowheads mark T-DNA insertion sites. T-DNAs are inserted 137 bp and 218 bp upstream of the stem-loops of MIR156A and MIR156C, respectively. (B) Expression …
Figure 2 with 1 supplement
Expression of miR156.
(A and B) Accumulation of miR156 in 8-, 9-, and 16-day-old long day plants. Expression of miR156 was analyzed by small RNA blot (A) and qRT-PCR (B). The plants were collected at Zeitgeber time (ZT) …
Figure 2—figure supplement 1
Expression pattern of miR156.
Expression of miR156. The plants were collected every 4 hr and subjected to qRT-PCR analyses. ZT: Zeitgeber time.
Figure 3 with 1 supplement
Sugar represses miR156.
(A) Expression of miR156, pri-MIR156A, and pri-MIR156C in response to sugar. Five-day-old wild type seedlings in 1/2 Murashige and Skoog (MS) liquid media were treated with 50 mM sucrose (Suc), …
Figure 3—figure supplement 1
Sugar represses miR156.
Accumulation of miR156 in response to sugar. Five-day-old wild type seedlings were treated with sugar for 1 day and subjected to RNA blot analyses. U6 was monitored as an internal control. Sugar …
Figure 4
Sugar as a mobile signal to trigger vegetative phase transition.
(A) Expression of miR156 in 15-day-old and 60-day-old wild type plants grown under short day conditions. (B) Sugar measurement. Fifteen-day-old and 60-day-old short day plants were collected at …
Figure 5 with 1 supplement
cao/ch1 mutant impairs vegetative phase transition.
(A) Leaf morphology of wild type, cao/ch1, and 35S::MIM156 cao/ch1 plants under long day conditions. The leaves from 15-day-old plants were detached and scanned. The numbers indicate leaf positions. …
Figure 5—figure supplement 1
Phenotype of cao mutant.
Plant morphology of wild type (wt), cao/ch1, and 35S::MIM156 cao/ch1. Scale bar indicates 1.0 cm.
Figure 6
Repression of miR156 by sugar is evolutionarily conserved.
Expression of miR156 in Physcomitrella patens, Solanum lycopersicum, and Nicotiana benthamiana. The plants were treated with 50 mM sucrose (Suc) or mannitol (Man) for 2 days. U6 was monitored as the …
Figure 7 with 2 supplements
Sugar promotes the degradation of miR156 primary transcripts.
(A) Chromatin immunoprecipitation (ChIP) analyses. Five-day-old wild type seedlings were treated with 50 mM glucose (Glc) or mannitol (Man) for 1 day. Anti-Pol II was used for ChIP analyses. The …
Figure 7—figure supplement 1
Effect of CHX on sugar-induced pri-MIR156C degradation.
Five-day-old wild type seedlings in 1/2 Murashige and Skoog liquid media were pre-treated with actinomycin-D (ActD) for 12 hr. Glucose was added 1 h after 100 µM cycloheximide (CHX). The levels in …
Figure 7—figure supplement 2
Expression analyses of pri-MIR156A and pri-MIR156C in upf mutants.
(A) Expression of pri-MIR156A and pri-MIR156C in upf1-5 and upf3-1 mutants. Seven-day-old wild type (WT), upf1-5, and upf3-1 seedlings were used for expression analyses. (B) Glucose response in upf …
Figure 8
The role of HXK1 in sugar-induced miR156 repression.
(A) Expression of miR156 in the 5-day-old wild type (ecotype Ler) and gin2-1 mutant. The expression level of miR156 in Ler was set to 1. (B) Time course analyses of miR156 in the gin2-1 mutant. (C …
Additional files
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Supplementary file 1
(A) MIR156 T-DNA insertion mutants. (B) Oligonucleotide primer sequences.
- https://doi.org/10.7554/eLife.00269.016
