1. Genetics and Genomics
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Genome Engineering: Bacteria herald a new era of gene editing

  1. David J Segal  Is a corresponding author
  1. University of California, Davis, United States
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Cite this article as: eLife 2013;2:e00563 doi: 10.7554/eLife.00563
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Figures

Jinek et al. attached a Cas9 nuclease (represented here by the purple rounded rectangle; the white arrows are the endonucleases) to a single-guide RNA (sgRNA), which guided it to a specific region of the target DNA called the protospacer.

The sgRNA is a hybrid of two components: CRISPR RNA (crRNA; blue) and trans-activating crRNA (tracrRNA; red). (A) Cleavage of the target DNA by the Cas9 nuclease results in mutations that can knock out the target gene. (B) If an appropriate donor DNA molecule is available, genetic information (shown here in pale blue) can be added to the target DNA in a precise manner. (C) By targeting two Cas9 nucleases to different regions of the target DNA, it is possible to delete the genetic information between the two regions. CRISPR: clustered regularly interspaced short palindromic repeats; Cas: CRISPR-associated.

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