(A) Histogram of fold changes of gene expression in sporocysts 48 hr post-transformation with respect to expression in miracidia, follows a log-normal distribution. The half standard deviation is used as the threshold to define differentially expressed transcripts. (B) Expression of sporocyst-specific genes that have homologs in neoblast-enriched transcripts is plotted logarithmically in fold change vs RPKM. A group of well-characterized conserved stem cell/neoblast markers, including ago2-1, vlg-3, and fgfrA, listed in Table 1, is highlighted by open symbols. (C) Confocal sections of sporocysts 24 hr post-transformation showing the expression of various conserved neoblast genes which overlap with the expression of cell cycle-associated transcripts. From top to bottom: mago nashi homolog (Smp_103470), far upstream (fuse) binding protein (Smp_044550), nucleosome assembly protein (nap, Smp_023530), adenosylhomocysteinase (ahcy, Smp_028440), pescadillo related gene (Smp_055800), and p30 dbc protein (Smp_193440).