1. Cell Biology
  2. Plant Biology
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Natural variation reveals that intracellular distribution of ELF3 protein is associated with function in the circadian clock

  1. M. Usman Anwer
  2. Eleni Boikoglou
  3. Eva Herrero
  4. Marc Hallstein
  5. Amanda M Davis
  6. Geo Velikkakam James
  7. Ferenc Nagy
  8. Seth J Davis  Is a corresponding author
  1. Max Planck Institute for Plant Breeding Research, Germany
  2. Cold Spring Harbour Laboratory, Germany
  3. MRC National Institute for Medical Research, United Kingdom
  4. Biological Research Centre of the Hungarian Academy of Sciences, Hungary
Research Article
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Cite this article as: eLife 2014;3:e02206 doi: 10.7554/eLife.02206

Abstract

Natural selection of variants within the Arabidopsis thaliana circadian clock can be attributed to adaptation to varying environments. To define a basis for such variation, we examined clock speed in a reporter-modified Bay-0 x Shakdara recombinant inbred line and localized heritable variation. Extensive variation led us to identify EARLY FLOWERING3 (ELF3) as a major quantitative trait locus (QTL). The causal nucleotide polymorphism caused a short-period phenotype under light and severely dampened rhythm generation in darkness, and entrainment alterations resulted. We found that ELF3-Sha protein failed to properly localize to the nucleus, and its ability to accumulate in darkness was compromised. Evidence was provided that the ELF3-Sha allele originated in Central Asia. Collectively we showed that ELF3 protein plays a vital role in defining its light-repressor action in the circadian clock and that its functional abilities are largely dependent on its cellular localization.

Article and author information

Author details

  1. M. Usman Anwer

    Max Planck Institute for Plant Breeding Research, Cologne, Germany
    Competing interests
    The authors declare that no competing interests exist.
  2. Eleni Boikoglou

    Cold Spring Harbour Laboratory, Laurel Hollow, Germany
    Competing interests
    The authors declare that no competing interests exist.
  3. Eva Herrero

    MRC National Institute for Medical Research, London, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
  4. Marc Hallstein

    Max Planck Institute for Plant Breeding Research, Cologne, Germany
    Competing interests
    The authors declare that no competing interests exist.
  5. Amanda M Davis

    Max Planck Institute for Plant Breeding Research, Cologne, Germany
    Competing interests
    The authors declare that no competing interests exist.
  6. Geo Velikkakam James

    Max Planck Institute for Plant Breeding Research, Cologne, Germany
    Competing interests
    The authors declare that no competing interests exist.
  7. Ferenc Nagy

    Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary
    Competing interests
    The authors declare that no competing interests exist.
  8. Seth J Davis

    Max Planck Institute for Plant Breeding Research, Cologne, Germany
    For correspondence
    seth.davis@york.ac.uk
    Competing interests
    The authors declare that no competing interests exist.

Reviewing Editor

  1. Todd C Mockler, Donald Danforth Plant Science Center, United States

Publication history

  1. Received: January 3, 2014
  2. Accepted: May 22, 2014
  3. Accepted Manuscript published: May 27, 2014 (version 1)
  4. Version of Record published: July 1, 2014 (version 2)

Copyright

© 2014, Anwer et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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