Gene models of Tu-CAS and its neighboring genes are depicted as follows: blue and red rectangles represent coding sequences and untranslated regions, respectively, while introns are shown as dashed lines. (+) and (−) represent the forward and reverse strand, respectively. Underneath the gene models, indicated in green, are the length and position of amplicons obtained by PCR (Figure 4). Next, an alignment of paired-end Sanger reads (and corresponding coverage plot) with the T. urticae genome of the London strain is displayed. Paired-end Sanger reads for which both reads are mapped in or extend nearby the indicated region are denoted by thin lines to show pair connections (shown are all paired-end Sanger reads that were produced from 2.5, 8.5, and 35.5 kb libraries used for assembly of the T. urticae genome [Grbic et al., 2011]). The Sanger reads coverage plot is followed by coverage plots of Illumina-reads from genomic DNA sequencing of the Montpellier and EtoxR strain of T. urticae (Grbic et al., 2011; Van Leeuwen et al., 2012). The coverage plot at the bottom shows Illumina RNA-seq read coverage produced from adult T. urticae polyA selected RNA (Grbic et al., 2011). Numbers between brackets represent the sequence depth.