(A) MCF10A cells treated with indicated siRNAs for 48 hr were analyzed for entosis (n = 3 ± SD analyzed by one way ANOVA followed by Dunnett's post-tests compared with siMOCK group). (B) MCF10A cells stably expressing mCherry-H2B or GFP-H2B were treated with indicated siRNAs before equal cell numbers were mixed and plated to analyze entotic invasion. (C) HEK293 cells were transfected with LPAR2 cDNA before analyzation for entosis (n = 3 ± SD, p<0.05, t test). (D) Immunolabeling of endogenous LPAR2 (red) and nuclei (DAPI) of MCF10A cells fixed at different stages during entosis as indicated. Scale bar 5 μm. (E) Immunolabeling of transfected Flag-tagged LPAR2 (green), F-actin (phalloidin, red), and nuclei (DAPI) of invading HEK293 cells undergoing entosis with or without 5 min addition of 100 nM latrunculin B (LatB) before fixation. Arrows indicate disassembled F-actin. Scale bar 5 μm.