(A, A′, C) Charts depict LTP positive neurons/brain hemisphere (A) and Repo positive glia/brain (A′), and DRNs or LTP positive DRNs/brain (C) from larvae reared on YF, PF, raised on PF till late second instar and transferred for 16hr on YF, and raised on YF till late second instar and transferred for 16 hr on PF. p-values (Student's ttest) are indicated, * = p < 0.05, ** = p < 0.01, n.s. = not significant, error bars = standard deviation. (B) Equal volumes hemolymph from early third instar wt larvae reared on YF or PF probed for Apo-LTP, Apo-LPP and Cvd. (D, E, G) Plotted are percentages (Y-axis) of hatched embryos (D; nYF = 198, nPF = 198; parental flies kept for three generations on respective food types before embryo collection), pupariated larvae (E; nYF = 163 and nPF = 133; time of pupal development was unchanged) and of living mated females (G; nYF = 40 and nPF = 35) over time (X-axis). Please note, LD50 indicated with grey line (G). (F–F″) Exemplary photographs of staged wt larvae bred on YF (left) or PF (right). Hours after egg collection (AEC) are indicated in top right corner.