(A) Schematic representation of the experimental geometry (not to scale). The MT suspended bridge is formed by attaching an MT (green) to the surface-immobilized beads (gray) that are coated with a …
(A) Example trace of a kinesin-1 coated bead, traveling along GMP-CPP MT filaments with a left handed spiraling motion. (B) Representative two dimensional trace. The average pitch of helical motion …
(A and B) (top) Representative three-dimensional trace of a cargo bead-driven by GST-Dyn331kD motors shows left- (A) and right-handed (B) helical motion. (bottom) Two-dimensional projections of the …
(A) First 20 s of the 3D trace shown in Figure 2A is plotted in x, y, and z directions as a function of time. (B) The brightfield image of the bead shows the changes in the position and intensity of …
(top) Representative three-dimensional trace of a cargo bead-driven by GST-Dyn331kD motors shows right-handed helical motion. (bottom) Two-dimensional projection of the trace shown at top.
(A and B) (top) Representative three-dimensional trace of a cargo bead driven by full-length dynein motors shows left- (A) and right-handed (B) helical motion. (bottom) Two-dimensional projections …
An example trace shows that a cargo bead initially moves along a GMP-CPP MT bridge with a right-handed helical motion. At around t = 5 s, the bead reverses its helical directionality (arrow) and …
(A) Representative two-dimensional trace of cargo beads driven by GST-Dyn331kD on taxol stabilized MTs which contains the mixture of 12, 13, 14 protofilaments with different rotational pitches. (B) …
(A) Schematic representation of quantum-dot labeled single dynein motors on the MT bridges (not to scale). Expected amplitude of rotations is ∼50 nm. (B) Two example traces show 2D projection of …
(A) Representative three-dimensional trace of a cargo bead driven by monomers shows right-handed helical motion. (B) (top) Representative two-dimensional trace for monomeric Dyn331kD. (bottom) …
(A) Top view of a monomeric dynein (red oval) stepping toward the MT-minus end (arrows). The yellow circles represent the putative binding sites for the highlighted dyneins. The closest available …
Matlab code used in data analysis.