1. Structural Biology and Molecular Biophysics
Download icon

Conserved mechanisms of microtubule-stimulated ADP release, ATP binding, and force generation in transport kinesins

  1. Joseph Atherton
  2. Irene Farabella
  3. I-Mei Yu
  4. Steven S Rosenfeld
  5. Anne Houdusse
  6. Maya Topf
  7. Carolyn A Moores  Is a corresponding author
  1. Birkbeck College, University of London, United Kingdom
  2. Institut Curie, Centre National de la Recherche Scientifique, France
  3. Lerner Research Institute, Cleveland Clinic, United States
Research Article
  • Cited 50
  • Views 2,993
  • Annotations
Cite this article as: eLife 2014;3:e03680 doi: 10.7554/eLife.03680

Abstract

Kinesins are a superfamily of microtubule-based ATP-powered motors, important for multiple, essential cellular functions. How microtubule binding stimulates their ATPase and controls force generation is not understood. To address this fundamental question, we visualized microtubule-bound kinesin-1 and kinesin-3 motor domains at multiple steps in their ATPase cycles - including their nucleotide-free states - at ~7Å resolution using cryo-electron microscopy. In both motors, microtubule binding promotes ordered conformations of conserved loops that stimulate ADP release, enhance microtubule affinity and prime the catalytic site for ATP binding. ATP binding causes only small shifts of these nucleotide-coordinating loops but induces large conformational changes elsewhere that allow force generation and neck linker docking towards the microtubule plus end. Family-specific differences across the kinesin-microtubule interface account for the distinctive properties of each motor. Our data thus provide evidence for a conserved ATP-driven mechanism for kinesins and reveal the critical mechanistic contribution of the microtubule interface.

Article and author information

Author details

  1. Joseph Atherton

    Birkbeck College, University of London, London, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
  2. Irene Farabella

    Birkbeck College, University of London, London, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
  3. I-Mei Yu

    Institut Curie, Centre National de la Recherche Scientifique, Paris, France
    Competing interests
    The authors declare that no competing interests exist.
  4. Steven S Rosenfeld

    Lerner Research Institute, Cleveland Clinic, Cleveland, United States
    Competing interests
    The authors declare that no competing interests exist.
  5. Anne Houdusse

    Institut Curie, Centre National de la Recherche Scientifique, Paris, France
    Competing interests
    The authors declare that no competing interests exist.
  6. Maya Topf

    Birkbeck College, University of London, London, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
  7. Carolyn A Moores

    Birkbeck College, University of London, London, United Kingdom
    For correspondence
    c.moores@mail.cryst.bbk.ac.uk
    Competing interests
    The authors declare that no competing interests exist.

Reviewing Editor

  1. Anthony A Hyman, Max Planck Institute of Molecular Cell Biology and Genetics, Germany

Publication history

  1. Received: June 13, 2014
  2. Accepted: September 8, 2014
  3. Accepted Manuscript published: September 10, 2014 (version 1)
  4. Accepted Manuscript updated: September 17, 2014 (version 2)
  5. Version of Record published: October 8, 2014 (version 3)

Copyright

© 2014, Atherton et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 2,993
    Page views
  • 398
    Downloads
  • 50
    Citations

Article citation count generated by polling the highest count across the following sources: Crossref, Scopus, PubMed Central.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Download citations (links to download the citations from this article in formats compatible with various reference manager tools)

Open citations (links to open the citations from this article in various online reference manager services)

Further reading

    1. Structural Biology and Molecular Biophysics
    Alison B Hickman et al.
    Research Article Updated
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics
    James Chen et al.
    Research Article Updated