(A–C) document a MARCM clonal brain, in which the clones null for otd function and are labelled by the GH146-Gal4. In this brain, the three known antennal lobe lineages normally labelled by GH146-Gal4 (ALad1, ALl1, and Alv1) have been recovered, along with an additional fourth neuroblast lineage, LALv1. (D–G) document a control brain where Otd is not down regulated in the LALv1 lineage (yellow dotted lines in E). In this brain, there are three clusters of antennal lobe projection neurons labelled by GH146-QF corresponding to the ALad1, ALl1 and ALv1 lineages, magenta dotted lines in D and G). (H–K) show a brain in which there has been efficient knock down of Otd in the LALv1 lineage (note loss of Otd immunolabelling ventral to the antennal lobe; yellow dotted lines in I). In this brain, apart from the ALad1, ALl1, and ALv1 projection neurons, an additional, fourth cluster of cells is seen innervating the antennal lobe (‘4’, LALv1). The yellow asterisks in A, D, H indicate the point of entry of the ALv1 lineage into the antennal lobe, and the yellow arrow indicates its axon tract. The magenta asterisks in A, D, H indicate the point of entry of the LALv1 lineage into the antennal lobe, and the magenta arrow indicates its axon tract. Note that these are distinct from each other. Genotype in A–C: FRT19A,otdYH13/FRT19A,Tub-Gal80,hsFLP; GH146-Gal4,UAS-mCD8::GFP/+. Genotype in D–K: UASdicer/+;Insc-Gal4/UAS-miRNA-otd-1;GH146-QF,QUAS-mtdTomato-HA/+). Scale bars are 50 µm. The one in A is applicable to B and C and the one in in D is applicable to D–K. Yellow line represents the midline.