LNKN-1::YFP localizes to the plasma membrane. In addition to the male somatic gonad (Figure 3E,F), expression was seen in: (A and B) seam cells, (C and D) pharynx (bracket), pharyngeal-intestinal valve (arrow), and excretory cell (arrowhead), (E and F) hermaphrodite somatic gonad (bracket) and vulval precursor cells (arrows), (G and H) the hermaphrodite distal tip cell, (I and J) intestine (bracket), male hook precursor cells (arrowhead), and tail cells (arrow), and (K and L) excretory canal (arrows, longitudinal fluorescent line) and intestine in the background. (M and N) YFP fused to the beginning of the extracellular domain of LNKN-1, in YFP::LNKN-1 constructs, also shows the same plasma membrane localization. Bracket marks the male somatic gonad. (O and P) Dissected, fixed gonad from LNKN-1::YFP expressing animals (O) and the same gonad stained with DLG-1 antibody, an apical domain marker. LNKN-1::YFP is expressed uniformly in the plasma membrane and does not show enrichment in the apical domain as marked by DLG-1 antibody. In contrast, LNKN-1 antibody staining shows enriched LNKN-1 localization to the apical domain (Figure 3A–D). In all figures, the top panel is a Nomarski micrograph and the bottom panel is an epifluorescence image of the same region. Scale bars represent 10 μm except in I and J, where they represent 20 μm. Anterior is left, posterior is right, dorsal is up, and ventral is down.