Quantitative system drift compensates for altered maternal inputs to the gap gene network of the scuttle fly Megaselia abdita

This table shows the number of embryos used to calculate median positions for each expression boundary at each point in time (M. abdita: white rows; D. melanogaster: grey rows). Ant: anterior, Post: posterior domain. A indicates anterior, P posterior boundary of a domain. Time classification as defined in Wotton et al. (2014) for M. abdita and in Surkova et al. (2008b) for D. melanogaster: C11–13 correspond to cleavage cycles 11 to 13; T1–8 represent time classes subdividing C14A. Our M. abdita expression dataset consists of a total of 367 embryos (91 stained for hb, 83 for Kr, 87 for gt, and 106 for kni). An additional 115 embryos make up the dataset for maternal co-ordinate and terminal gap genes shown in Supplementary file 1. The D. melanogaster gap gene dataset has been published previously (Crombach et al., 2012a). It is included here for comparison.

A total of 637 RNAi-treated embryos were used for the analysis. This table shows the number of embryos that were stained for each of the trunk gap genes (rows), in each RNAi-treated background (columns), and the number of embryos that showed a knock-down phenotype (see also percentages). The total number of embryos used for each knock-down experiment is shown in the bottom row. A more detailed breakdown of embryos per cleavage cycle and time class, including detailed plots of boundary positions, is provided in Supplementary file 4.