A highly-tunable dopaminergic oscillator generates ultradian rhythms of behavioral arousal

  1. Ian D Blum
  2. Lei Zhu
  3. Luc Moquin
  4. Maia V Kokoeva
  5. Alain Gratton
  6. Bruno Giros
  7. Kai-Florian Storch  Is a corresponding author
  1. McGill University, Canada
  2. Douglas Mental Health University Institute, Canada

Abstract

Ultradian (~4 h) rhythms in locomotor activity that do not depend on the master circadian pacemaker in the suprachiasmatic nucleus have been observed across mammalian species, however, the underlying mechanisms driving these rhythms are unknown. We show that disruption of the dopamine transporter gene lengthens the period of ultradian locomotor rhythms in mice. Period lengthening also results from chemogenetic activation of midbrain dopamine neurons and psychostimulant treatment, while the antipsychotic haloperidol has the opposite effect. We further reveal that striatal dopamine levels fluctuate in synchrony with ultradian activity cycles and that dopaminergic tone strongly predicts ultradian period. Our data indicate that an arousal regulating, dopaminergic ultradian oscillator (DUO) operates in the mammalian brain, which normally cycles in harmony with the circadian clock, but can desynchronize when dopamine tone is elevated, thereby producing aberrant patterns of arousal which are strikingly similar to perturbed sleep-wake cycles comorbid with psychopathology.

Article and author information

Author details

  1. Ian D Blum

    Department of Psychiatry, McGill University, Montreal, Canada
    Competing interests
    The authors declare that no competing interests exist.
  2. Lei Zhu

    Department of Psychiatry, McGill University, Montreal, Canada
    Competing interests
    The authors declare that no competing interests exist.
  3. Luc Moquin

    Douglas Mental Health University Institute, Montreal, Canada
    Competing interests
    The authors declare that no competing interests exist.
  4. Maia V Kokoeva

    Department of Medicine, McGill University, Montreal, Canada
    Competing interests
    The authors declare that no competing interests exist.
  5. Alain Gratton

    Department of Psychiatry, McGill University, Montreal, Canada
    Competing interests
    The authors declare that no competing interests exist.
  6. Bruno Giros

    Department of Psychiatry, McGill University, Montreal, Canada
    Competing interests
    The authors declare that no competing interests exist.
  7. Kai-Florian Storch

    Department of Psychiatry, McGill University, Montreal, Canada
    For correspondence
    florian.storch@mcgill.ca
    Competing interests
    The authors declare that no competing interests exist.

Ethics

Animal experimentation: All experimental procedures were performed in accordance with the Canadian Council on Animal Care guidelines and approved by the McGill University Animal Care Committee (animal use protocol #2010-5945).

Reviewing Editor

  1. Richard D Palmiter, Howard Hughes Medical Institute, University of Washington, United States

Publication history

  1. Received: October 10, 2014
  2. Accepted: December 28, 2014
  3. Accepted Manuscript published: December 29, 2014 (version 1)
  4. Version of Record published: February 12, 2015 (version 2)

Copyright

© 2014, Blum et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Ian D Blum
  2. Lei Zhu
  3. Luc Moquin
  4. Maia V Kokoeva
  5. Alain Gratton
  6. Bruno Giros
  7. Kai-Florian Storch
(2014)
A highly-tunable dopaminergic oscillator generates ultradian rhythms of behavioral arousal
eLife 3:e05105.
https://doi.org/10.7554/eLife.05105

Further reading

  1. Dopamine drives an "ultradian" clock with a period of around four hours in mice.

    1. Neuroscience
    Lindsay Collins, John Francis ... David A McCormick
    Research Article Updated

    Fluctuations in brain and behavioral state are supported by broadly projecting neuromodulatory systems. In this study, we use mesoscale two-photon calcium imaging to examine spontaneous activity of cholinergic and noradrenergic axons in awake mice in order to determine the interaction between arousal/movement state transitions and neuromodulatory activity across the dorsal cortex at distances separated by up to 4 mm. We confirm that GCaMP6s activity within axonal projections of both basal forebrain cholinergic and locus coeruleus noradrenergic neurons track arousal, indexed as pupil diameter, and changes in behavioral engagement, as reflected by bouts of whisker movement and/or locomotion. The broad coordination in activity between even distant axonal segments indicates that both of these systems can communicate, in part, through a global signal, especially in relation to changes in behavioral state. In addition to this broadly coordinated activity, we also find evidence that a subpopulation of both cholinergic and noradrenergic axons may exhibit heterogeneity in activity that appears to be independent of our measures of behavioral state. By monitoring the activity of cholinergic interneurons in the cortex, we found that a subpopulation of these cells also exhibit state-dependent (arousal/movement) activity. These results demonstrate that cholinergic and noradrenergic systems provide a prominent and broadly synchronized signal related to behavioral state, and therefore may contribute to state-dependent cortical activity and excitability.