1. Cell Biology

Controlling fertilization and cAMP signaling in sperm by optogenetics

  1. Vera Jansen
  2. Luis Alvarez
  3. Melanie Balbach
  4. Timo Strünker
  5. Peter Hegemann
  6. U Benjamin Kaupp  Is a corresponding author
  7. Dagmar Wachten  Is a corresponding author
  1. Center of Advanced European Studies and Research, Germany
  2. Humboldt University of Berlin, Germany
https://doi.org/10.7554/eLife.05161
Share this article
Cite this article
  1. Vera Jansen
  2. Luis Alvarez
  3. Melanie Balbach
  4. Timo Strünker
  5. Peter Hegemann
  6. U Benjamin Kaupp
  7. Dagmar Wachten
(2015)
Controlling fertilization and cAMP signaling in sperm by optogenetics
eLife 4:e05161.
https://doi.org/10.7554/eLife.05161
  2. Download BibTeX
  3. Download .RIS

Abstract

Optogenetics is a powerful technique to control cellular activity by light. The light-gated Channelrhodopsin has been widely used to study and manipulate neuronal activity in vivo, whereas optogenetic control of second messengers in vivo has not been examined in depth. In this study, we present a transgenic mouse model expressing a photoactivated adenylyl cyclase (bPAC) in sperm. In transgenic sperm, bPAC mimics the action of the endogenous soluble adenylyl cyclase (SACY) that is required for motility and fertilization: light-stimulation rapidly elevates cAMP, accelerates the flagellar beat, and, thereby, changes swimming behavior of sperm. Furthermore, bPAC replaces endogenous adenylyl cyclase activity. In mutant sperm lacking the bicarbonate-stimulated SACY activity, bPAC restored motility after light-stimulation and, thereby, enabled sperm to fertilize oocytes in vitro. We show that optogenetic control of cAMP in vivo allows to non-invasively study cAMP signaling, to control behaviors of single cells, and to restore a fundamental biological process such as fertilization.

https://doi.org/10.7554/eLife.05161.001

eLife digest

Tiny hair-like structures called cilia on the outside of cells play many important roles, including detecting physical and chemical signals from the environment. Special cilia—called flagella—help cells to move around and perhaps the most well-known of these are sperm flagella, which propel sperm in their quest to fertilize the egg. A chemical messenger called cAMP is essential for the movement of sperm flagella.

When a sperm cell enters the female reproductive tract, an enzyme called SACY is activated. Within seconds, SACY produces cAMP and, thereby, causes the flagella to beat faster so that the sperm cell speeds toward the egg. cAMP also controls sperm maturation, which is needed to penetrate the egg. However, the precise details of the role of cAMP in sperm cells are not clear.

Here, Jansen et al. have investigated this role using a cutting-edge technique—called optogenetics—that was originally developed to study brain cells in living organisms. Jansen et al. genetically engineered a mouse so that exposing sperm to blue light activates a light-sensitive enzyme called bPAC that increases cAMP levels in sperm.

In these mice, the activation of bPAC by light accelerated the beating of the flagella so the sperm moved faster, in a way that was similar to the effects that are normally observed after the activation of the SACY enzyme. In mice lacking among other things the SACY enzyme—whose sperm cells are unable to move or fertilize an egg—activating the light-sensitive bPAC enzyme restored sperm motility and enabled the sperm to fertilize an egg.

These results show that optogenetics may be a useful tool for studying how flagella and other types of cilia work.

https://doi.org/10.7554/eLife.05161.002

Introduction

Almost every eukaryotic cell contains a specialized surface protrusion called the primary cilium (Singla and Reiter, 2006). Primary cilia serve as sensory antennae that register physical and chemical cues from the environment. Cilia are not only involved in for example, mechano-, chemo-, and photo-sensation but also in embryonic and neuronal development (Praetorius and Spring 2001; Singla and Reiter, 2006; Insinna and Besharse, 2008; Goetz and Anderson, 2010; Satir et al., 2010). Motile cilia, also called flagella, are used both as sensory antenna and motors that move fluids or propel cells (Salathe, 2007; Bloodgood, 2010; Lindemann and Lesich, 2010; Pichlo et al., 2014). Signaling in cilia and flagella is tightly regulated, spatially confined, and relies on a cilia-specific transport machinery (Rosenbaum and Witman, 2002; Delling et al., 2013; Chung et al., 2014; Pichlo et al., 2014).

Cyclic nucleotide-dependent signaling plays a central role in both primary and motile cilia. In ciliary structures of olfactory neurons and photoreceptors, it regulates chemosensation and photoreception, respectively (Johnson and Leroux, 2010). In cilia of endothelial and mesenchymal cells, cAMP signaling controls cilia length in response to extracellular stimuli (Besschetnova et al., 2010). Defects in cilia of the renal epithelium cause polycystic kidney disease (Pazour et al., 2000). During embryonic development, ciliary cAMP signaling is involved in neural tube development by regulating the Sonic hedgehog (Shh) pathway (Mukhopadhyay et al., 2013).

A case in point for the importance of cAMP signaling in flagella is the mammalian sperm cell. Cyclic AMP signaling is essential for sperm development, motility, and maturation in the female genital tract (Visconti et al., 1995b; Wennemuth et al., 2003; Krähling et al., 2013). However, the underlying signaling pathways are not well understood. CRIS, a cAMP-binding protein, controls spermatogenesis and sperm motility (Krähling et al., 2013). An increase in bicarbonate (HCO3−) during the transit from the epididymis to the female genital tract activates a soluble adenylyl cyclase (SACY) and, thereby, accelerates the flagellar beat and enhances progressive sperm motility (Esposito et al., 2004; Xie et al., 2006). HCO3−-induced cAMP synthesis by SACY stimulates protein kinase A (PKA) (Wennemuth et al., 2003; Nolan et al., 2004) and controls capacitation, a maturation process of sperm in the female genital tract that is essential for fertilization (Chang, 1951; Austin, 1952). Acceleration of the flagellar beat and capacitation proceed on quite different time scales: the beat frequency increases within seconds, whereas PKA-dependent tyrosine phosphorylation, a hallmark of sperm capacitation, takes about an hour to become noticeable (Visconti et al., 1995a).

The study of cAMP-dependent signaling pathways in mammalian sperm is further complicated by an ill-defined interplay between cAMP and Ca2+ signaling; for example, Ca2+ controls SACY activity (Carlson et al., 2007) and cAMP reportedly evokes a Ca2+ influx (Kobori et al., 2000; Ren et al., 2001; Xia et al., 2007; Wertheimer et al., 2013). Previous attempts to delineate these signaling pathways relied on common pharmacological tools: membrane-permeable cAMP analogs and modulators of cAMP signaling components (Kobori et al., 2000; Xia et al., 2007; Wertheimer et al., 2013). Recent studies in human sperm, however, refute that cAMP controls [Ca2+]i (Strünker et al., 2011) and disclose serious shortcomings of these pharmacological tools that directly act on the sperm Ca2+ channel CatSper and, thereby, artifactually change Ca2+ levels (Brenker et al., 2012). In mice, however, it is equivocal whether cAMP indeed evokes a Ca2+ signal. Therefore, it is required to study the interconnection of cAMP and Ca2+ signaling in mouse sperm by novel, non-invasive techniques.

Optogenetics is a powerful tool to spatially and temporally control second messenger-dependent signaling, uncompromised by pharmacological side effects. To this end, we present here a transgenic mouse model expressing the photoactivated adenylyl cyclase bPAC (Ryu et al., 2010; Stierl et al., 2011) to manipulate cAMP levels in sperm and, thereby, control sperm motility by light. We show that bPAC mimics the action of HCO3− in sperm. Furthermore, bPAC functionally replaces the endogenous SACY activity and remedies cAMP signaling defects, demonstrating that in vitro, fertility can be restored by optogenetics. Finally, an increase of cAMP levels by light does not elevate Ca2+ levels in sperm. Thus, the Prm1-bPAC mouse model is a powerful tool to analyze cAMP-dependent signaling in ciliary structures with high spatio-temporal resolution uncompromised by pharmacological artifacts.

Results

Generation of Prm1-bPAC transgenic mice

We engineered a targeting vector to express the beta subunit of photo activated adenylyl cyclase from the soil bacterium Beggiatoa (bPAC) under the control of the protamine 1 promoter (Prm1, Figure 1A) that is exclusively active in post-meiotic spermatids (Zambrowicz et al., 1993). Transgenic mice were generated by pronuclear injection using standard procedures (Ittner and Götz, 2007). Genomic insertion of the transgene was confirmed by PCR (Figure 1B). Protein expression of bPAC in testis lysates varied between different founder lines (Figure 1C). This variation in transgene expression reflects differences in integration site and/or copy number. For further analysis, founder lines 1 and 5 were chosen due to stable inheritance of the transgene to the next generation. The bPAC protein was exclusively expressed in sperm (Figure 1D–F). Prm1-bPAC males were fertile and did not show any defects during spermatogenesis, demonstrating that bPAC expression does not affect sperm development or function (Figure 1—source data 1).

Figure 1
Download asset Open asset
Characterization of the Prm1-bPAC mouse.

(A) Scheme of the Prm1-bPAC targeting vector. Expression of hemagglutinin (HA)-tagged bPAC is driven by the protamine 1 promoter (Prm1); arrows indicate the position of genotyping primers. (B) Genotyping by PCR. In Prm1-bPAC mice, a 213-bp fragment is amplified. The targeting vector served as a positive control (+). (C) Western blot analyzing bPAC-HA expression in testis lysates from different founder lines. Lysates from HEK cells expressing bPAC-HA served as positive control (+), wild-type testis lysates as negative control (−). (D) Western blot analyzing bPAC-HA expression in tissue lysates from male and female Prm1-bPAC mice. (E) Western blot analyzing bPAC-HA expression in testis and sperm. (F) Immunohistochemical analysis of bPAC-HA expression (left panel: transmission, right panel: fluorescence). Pictures at the bottom show a higher magnification (see white box). Sperm flagella are indicated (arrow). Cryosections of mouse testis were probed with anti-HA antibody and fluorescent secondary antibody (green), DNA was stained with DAPI (blue). Loading control for Western blots: β-tubulin.

https://doi.org/10.7554/eLife.05161.003
Figure 1—source data 1

The Prm1-bPAC mouse model shows no change in fertility parameters.

Data are given as mean ± s.d.; n = number of experiments.

https://doi.org/10.7554/eLife.05161.004

Control of sperm cAMP levels and motility by light

To scrutinize whether bPAC allows optogenetic control of sperm cAMP signaling, we determined cAMP levels in whole sperm before and after light stimulation. Basal cAMP levels in bPAC sperm were slightly enhanced (bPAC: 15.3 ± 3.6 fmol/105 sperm versus wild-type: 10.0 ± 4.4 fmol/105 sperm; Figure 2A), which might be due to basal bPAC activity (Stierl et al., 2011). In wild-type sperm, light stimulation did not alter cAMP levels, whereas in bPAC sperm, cAMP levels increased by about 2.5-fold (Figure 2A). During continuous light stimulation, cAMP levels reached a maximum within 2 min (Figure 2B); after switching off the light, cAMP returned to baseline within 10 min (Figure 2B). The light-induced cAMP increase was similar to the HCO3−-induced cAMP increase (Figure 2A), indicating that activation of bPAC mimics the activation of SACY by HCO3−.

Figure 2
Download asset Open asset
Manipulation of cAMP signaling and sperm motility by light.

(A) Light stimulation of bPAC sperm for 10 min increases cAMP levels. Stimulation of SACY activity with HCO3− (1 min) evokes the same response in wild-type and bPAC sperm. (B) cAMP levels in bPAC sperm during prolonged light stimulation and after switching off the light. (C) Western blot analyzing SACY and PKA expression in wild-type and bPAC sperm. (D) Phosphorylation of PKA targets in wild-type and bPAC sperm detected with an anti-phospho-(Ser/Thr) PKA substrate antibody (p-PKA). Wild-type sperm were stimulated with 25 mM HCO3−, bPAC sperm with HCO3− or light. (E) db-cAMP- (1 mM) and HCO3−-induced tyrosine phosphorylation in wild-type and bPAC sperm detected with an anti-phospho-tyrosine antibody (pY). (F) Acrosome-reaction assay. Percentage of sperm that has undergone the acrosome reaction under non-capacitating conditions (no HCO3−) before and after light-stimulation and under capacitating conditions (25 mM HCO3−). (G) Basal flagellar beat frequency in wild-type and bPAC sperm (individual values and mean ± s.d.). (H, I) Light-induced change in flagellar beat frequency of individual bPAC sperm. (J) Average change in flagellar beat frequency after light stimulation. (K) Change in flagellar beat frequency of bPAC sperm after global or local light stimulation of the flagellum for 100 ms; see also Video 3 and 4. Data are plotted as mean ± s.d.; (n) = number of experiments, n ≥ 5 if not stated otherwise; p values calculated using Student's t test. Loading control for Western blots: β-tubulin.

https://doi.org/10.7554/eLife.05161.005

Next, we studied whether bPAC expression interferes with cellular events controlled by cAMP, such as capacitation. Sperm are capacitated in vitro by incubation with a cholesterol acceptor (e.g., serum albumin) and HCO3− to stimulate cAMP synthesis via SACY (Visconti et al., 1995a; Xie et al., 2006). cAMP in turn activates PKA, the principal cAMP target in sperm. PKA activation indirectly promotes tyrosine phosphorylation of a subset of proteins, which is considered to be a hallmark of capacitation (Visconti et al., 1995a; Nolan et al., 2004).

The presence of bPAC did not affect SACY expression level (Figure 2C), consistent with the observation that HCO3−-stimulated cAMP synthesis is similar in wild-type and bPAC sperm (Figure 2A). The expression level of PKA also remained unchanged in bPAC sperm (Figure 2C). The phosphorylation of PKA targets under non-stimulated conditions was also not majorly different in bPAC sperm compared to wild-type sperm (Figure 2D). Furthermore, stimulation of bPAC sperm with either light or HCO3− both evoked phosphorylation of PKA targets (Figure 2D). Finally, we analyzed whether the capacitation-associated phosphorylation of tyrosine residues was altered: incubation with HCO3− or the cAMP analog dibutyryl-cAMP (db-cAMP) enhanced tyrosine phosphorylation to a similar extent in wild-type and bPAC sperm (Figure 2E). In summary, bPAC expression does not interfere with known cAMP-signaling events in sperm.

Next, we studied whether a cAMP increase evoked by light-stimulation of bPAC results in sperm capacitation. As a functional read-out for sperm capacitation, we used an acrosome-reaction assay (Figure 2F). Under non-capacitating conditions (without HCO3−) in the dark, the percentage of sperm undergoing the acrosome reaction was similar between wild-type and bPAC sperm (wild-type: 34.1% versus bPAC: 41.7%; Figure 2F). After light-stimulation, the number of sperm undergoing the acrosome reaction changed dramatically for bPAC sperm but remained unchanged for wild-type sperm (wild-type: 38.4% bPAC: 70.5%; Figure 2F). This demonstrates that a light-stimulated increase in cAMP levels results in sperm capacitation and that bPAC functionally mimics the activation of SACY by HCO3−.

In mammalian sperm, cAMP controls the flagellar beat (Wennemuth et al., 2003; Esposito et al., 2004; Nolan et al., 2004). The basal beat frequency of bPAC sperm was slightly lower compared to wild-type sperm (5.5 ± 2.5 Hz versus 8.0 ± 3.7 Hz; Figure 2G), which might be due to a cAMP-dependent feedback mechanism activated by elevated basal cAMP levels (Burton and McKnight, 2007). Light stimulation of bPAC sperm evoked a transient acceleration of the flagellar beat: a 200-ms light pulse increased the beat frequency within 10 s about 2.5-fold from 7 Hz to 18 Hz (Figure 2H; Videos 1,2). The acceleration persisted for at least 10 s and sperm returned to basal beat frequency within 30 s (Figure 2H). The light-stimulated acceleration of the flagellar beat was dose-dependent: the longer the light exposure, the faster the onset and higher the beat frequency (Figure 2I,J). Local illumination of a small part of the flagellum also enhanced the beat frequency, albeit the increase was smaller and its kinetics slower compared to global illumination (Figure 2K, Videos 3,4). Altogether, we conclude that (1) light activation of bPAC mimics the transient beat acceleration evoked by HCO3− stimulation of SACY (Wennemuth et al., 2003; Carlson et al., 2007), (2) the behavioral response of single cells can be modulated in a graded fashion, and that (3) bPAC is able to control cAMP signaling in the sperm flagellum with spatial precision.

Video 1
Download asset
Wild-type sperm before and after UV flash.

The cell was tethered to the glass surface by lowering the BSA concentration (0.3 mg/ml). The recording was performed using an epifluorescent microscope (IX71; Olympus) equipped with a dark-field condenser and a 10x objective (UPlanFL, NA 0.3; Olympus) and an additional 1.6× lenses. Frames were acquired at 200 fps using a CMOS camera (Dimax; PCO). Stimulation with UV light for 200 ms was achieved using a UV LED. For clarity, the video is played with 100 fps (half original speed).

https://doi.org/10.7554/eLife.05161.006
Video 2
Download asset
bPAC sperm before and after UV flash.

The recording was performed as described for Video 1.

https://doi.org/10.7554/eLife.05161.007
Video 3
Download asset
Local illumination of bPAC sperm.

The recording was performed as described for Video 1 using local illumination of the sperm flagellum for 100 ms; the video is played with 100 fps (half of the original speed). In this setting, the light flash is not visible and is, therefore, visualized with a circle. The analysis of the beat frequency is presented in Figure 2K.

https://doi.org/10.7554/eLife.05161.008
Video 4
Download asset
Local illumination of bPAC sperm.

Video 3 shown at higher intensity for the frames containing the light flash. This allows to visualize the light flash. The profile of the light flash is indicated in an inset. For clarity, the video speed was reduced to 10% of the frames containing the light flash.

https://doi.org/10.7554/eLife.05161.009

Light-stimulated cAMP synthesis does not affect intracellular Ca2+ levels

We tested whether a light-induced increase of cAMP evokes a Ca2+ influx into bPAC sperm. The use of a red-shifted fluorescent Ca2+ indicator enabled us to orthogonalize indicator excitation and bPAC activation (Stierl et al., 2011): after recording basal Ca2+ levels at 520 nm, we repeatedly switched between excitation of the Ca2+ indicator at 520 nm and activation of bPAC at 485 nm; finally, we challenged sperm with 8-Br-cNMPs or db-cAMP while exciting the Ca2+ indicator only (Figure 3A–C). To scrutinize our experimental conditions, we determined sperm cAMP levels before and after activation of bPAC (Figure 3D). We observed that cAMP synthesis via light stimulation of bPAC did not evoke a Ca2+ signal (Figure 3A–D), consistent with the observation that a HCO3−-induced cAMP increase does not affect [Ca2+]i in mouse (Wennemuth et al., 2003; Carlson et al., 2007) and human sperm (Strünker et al., 2011). In contrast, 8-Br-cAMP, 8-Br-cGMP, and db-cAMP evoked a Ca2+ signal in wild-type and bPAC sperm (Figure 3A–C,E) that was abolished in CatSper-null sperm (Figure 3F); similar results have been reported by others (Ren et al., 2001; Xia et al., 2007). In human sperm, 8-Br-cNMPs directly activate CatSper via an extracellular site (Brenker et al., 2012). Experiments analyzing the action of 8-Br-cNMPs on membrane currents in mouse sperm yielded inconsistent results (Kirichok et al., 2006; Cisneros-Mejorado et al., 2014). Thus, this effect needs to be addressed by future studies.

Figure 3
Download asset Open asset
cAMP does not evoke a Ca2+ influx in mouse sperm.

(A, B) Ca2+ signals induced by 8-Br-cAMP (10 mM; left) and db-cAMP (10 mM; right) in wild-type (A) and bPAC sperm (B) loaded with the fluorescent Ca2+ indicator FluoForte. Arrows indicate the addition of compounds. Light stimulation does not evoke a Ca2+ signal. Signals were measured in 96 multi-well plates in a fluorescence plate reader. (C) Mean signals evoked by light stimulation, cyclic nucleotide analogs, and ionomycin. (D) Illumination according to the protocol in (A, B) stimulates cAMP synthesis in bPAC sperm. Data are plotted as mean ± s.d.; (n) = number of experiments; p values calculated using Student's t test. (E, F) Ca2+ signals of capacitated sperm induced by cyclic nucleotide analogs (10 mM) in wild-type (E) and CatSper-null (F) sperm loaded with the fluorescent Ca2+ indicator Cal-520 in a stopped-flow device. Inset in (F): ionomycin control (2 µM).

https://doi.org/10.7554/eLife.05161.010

Light-stimulated cAMP synthesis restores fertility

The loss of SACY function abolishes cAMP signaling in sperm, rendering sperm immotile and resulting in male infertility (Esposito et al., 2004; Xie et al., 2006). We explored the potential of optogenetics to compensate for the functional loss of SACY. Removal of extracellular Ca2+ attenuated HCO3−-induced cAMP synthesis by SACY (Figure 4A) (Jaiswal and Conti, 2003; Carlson et al., 2007). Thereby, tyrosine phosphorylation in wild-type and bPAC sperm was largely abolished (Figure 4B). Elevation of basal cAMP levels by the phosphodiesterase inhibitor IBMX restored tyrosine phosphorylation in wild-type sperm (Figure 4A,B). Similarly, in Ca2+-free medium, the light-stimulated increase of cAMP levels restored tyrosine phosphorylation in bPAC sperm (Figure 4A,B), demonstrating that bPAC activation compensates for the loss of SACY activity. Furthermore, we examined whether in sperm lacking functional SACY, bPAC activation can restore motility and the ability to fertilize the egg. To this end, we crossed Prm1-bPAC mice with mice lacking the sperm-specific Na+/H+ exchanger sNHE, encoded by the Slc9a10 gene. Male Slc9a10-null mice are infertile, because their sperm are largely immotile (Wang et al., 2003). In Slc9a10-null mice, the HCO3−-stimulated SACY activity is abolished as well, probably because sNHE and SACY form a functional signaling complex that is disrupted in these mice (Wang et al., 2007). The motility of Slc9a10-null sperm is restored by db-cAMP (Wang et al., 2003); however, db-cAMP not only elevates cAMP levels but also evokes a Ca2+ influx via CatSper (Figure 3E). Thus, we tested whether motility can be restored by solely increasing cAMP levels with light. Sperm were immotile in darkness (Figure 4C, left); light stimulation restored both the flagellar beat and forward motility (Figure 4C, right; Video 5), demonstrating that restoring cAMP levels alone is sufficient to rescue motility. We went one step further and tested whether Slc9a10-null/bPAC sperm also regain their fertilization potential upon light stimulation. Indeed, light-stimulated Slc9a10-null/bPAC sperm were able to fertilize oocytes in vitro (Figure 4D), demonstrating that optogenetics can restore fertility.

Figure 4
Download asset Open asset
bPAC restores fertility in mice lacking functional SACY.

(A) Nominally Ca2+-free medium attenuates HCO3−-induced cAMP synthesis by SACY in wild-type and bPAC sperm. Inhibition of phosphodiesterases by IBMX in wild-type sperm and light stimulation in bPAC sperm increases cAMP levels without extracellular Ca2+. Data are plotted as mean ± s.d.; (n) = number of experiments; p values calculated using Student's t test. (B) In the absence of extracellular Ca2+, HCO3−-induced tyrosine phosphorylation (pY) is strongly attenuated. In bPAC sperm, light stimulation is sufficient to restore tyrosine phosphorylation. (C) Light stimulation restores flagellar beating of Slc9a10-null/bPAC sperm. Flagellar waveform of Slc9a10-null/bPAC sperm before (left) and after light stimulation (right). Successive, aligned, and superimposed images creating a ‘stop-motion’ image, illustrating one flagellar beating cycle. Scale bar: 30 µm. (D) Upon light stimulation, Slc9a10-null/bPAC sperm fertilize oocytes in vitro (mean ± s.d.; (n) = total number of oocytes from three independent experiments).

https://doi.org/10.7554/eLife.05161.011
Video 5
Download asset
Slc9a10-null/bPAC sperm before and after UV flash.

The recording was performed as described for Video 1, but without the additional 1.6× magnification, an acquisition frequency of 90 fps, and 500 ms UV stimulation. The video is shown in real time.

https://doi.org/10.7554/eLife.05161.012

Discussion

In this study, we report on a transgenic mouse model designed to control cAMP signaling in sperm using optogenetics. We demonstrate that transgenic expression of bPAC in sperm does not interfere with the endogenous HCO3−-stimulated SACY activity. In fact, bPAC mimics the effect of SACY activity: the light-stimulated increase in cAMP levels is similar to that activated by HCO3− and activation of bPAC increases the flagellar beat frequency in a dose-dependent manner. Thus, the Prm1-bPAC mouse model is an ideal optogenetic tool to study cAMP signaling in sperm.

Furthermore, we show that optogenetics can be used to restore fertility. Sperm from infertile Slc9a10-null mice are devoid of cAMP signaling, because they lack HCO3−-stimulated SACY activity, which is essential for sperm motility (Wang et al., 2003, 2007). Expression of bPAC in Slc9a10-null sperm allowed restoring motility after light stimulation and, most importantly, restoring fertilization in vitro. Of note, in vitro fertilization (IVF) using light-stimulated sperm was successful using zona pellucida-intact oocytes, demonstrating that a cAMP increase, at least in vitro, is sufficient to restore fertility in Slc9a10-null mice. In contrast, incubation of Slc9a10-null sperm with cAMP analogs only restored fertilization of zona pellucida-free but not zona pellucida-intact oocytes (Wang et al., 2003). This discrepancy could be explained by (1) cAMP analogs not reaching sufficiently high levels in sperm, or (2) cAMP analogs being not as efficient as cAMP to stimulate downstream targets, or (3) non-specific effects compromising the interpretation of their action (Brenker et al., 2012). The Prm1-bPAC mouse model holds great promise studying cAMP signaling uncompromised by side effects and allowed us to solve a long-standing controversy by showing that cAMP, in fact, does not stimulate a Ca2+ influx into mouse sperm (Figure 3B). These results demonstrate that optogenetics can disentangle cellular events such as Ca2+ and cAMP signaling in vivo.

Now that we have established the Prm1-bPAC mouse model and demonstrated that it can be used to analyze cAMP signaling in sperm, the next step will be to implement the spatio-temporal precision of this tool to study cAMP signaling in sperm. For example, HCO3− stimulation evokes a rapid cAMP increase and changes the flagellar beat frequency within seconds. However, PKA-dependent activation of tyrosine phosphorylation is barely detectable within the first hour of HCO3− incubation (Visconti et al., 1995a). Thus, HCO3− exhibits short-term and long-term effects and probably controls cellular events in addition to cAMP synthesis via SACY. The Prm1-bPAC mouse model allows deciphering these signaling events, if bPAC expression does not interfere with other signaling pathways.

Signaling molecules in sperm flagella are highly compartmentalized (Chung et al., 2014). The flagellar beat is controlled by cAMP-dependent phosphorylation of PKA targets in the axoneme (Salathe, 2007); however, it is unknown whether cAMP signaling is restricted to microdomains or whether changes in cAMP propagate along the flagellum. Compartmentalization of cAMP signaling confers specificity to this ubiquitous cellular messenger, for example, in cell-cycle progression or modulation of gene expression. (Michel and Scott, 2002; Zaccolo and Pozzan, 2002; Willoughby and Cooper, 2007). Our results show that local illumination of the flagellum of bPAC sperm is sufficient to increase its beat frequency. Optogenetics using bPAC provides the spatial and temporal precision to increase cAMP levels in subcellular regions and specialized signaling compartments such as cilia and flagella, which allows to study complex signaling networks - not only in mammalian sperm but also any other ciliated cell type.

Finally, the optogenetic toolkit has been recently expanded by a light-activated phosphodiesterase (LAPD) that hydrolyses cyclic nucleotides upon stimulation by red light (Gasser et al., 2014). A combination of LAPD and bPAC with fluorescent cAMP biosensors holds great promise to map the dynamics of cAMP signaling in live cells in precise spatio-temporal and quantitative terms. These findings encourage future studies to explore the full potential of controlling cellular messengers by optogenetics.

Materials and methods

Generation and genotyping of Prm1-bPAC transgenic mice

Request a detailed protocol

The bPAC cDNA (beta subunit of photo-activated adenylyl cyclase from Beggiatoa sp. PS, BGP_1043; modified for expression in mammalian systems according to Stierl et al. (2011), GU461307) sequence was amplified via PCR. A hemagglutinin (HA) tag was fused to the C terminus, an EcoRI restriction site was added to the 5′ end and a BamHI and XhoI restriction site to the 3′ end by nested PCR. The PCR product was cloned into a pBluescript SK(-) vector (Agilent Technologies, Santa Clara, USA) using EcoRI and XhoI (pB-bPAC). After sequencing, the bPAC-HA insert was excised and cloned into pPrCExV (a kind gift from Robert Braun, Jackson Laboratory) using EcoRI/BamHI. Transgenic mice were generated via pronuclear injection following standard procedures (Ittner and Götz, 2007) at the HET animal facility (University Hospital Bonn, Germany). Mice were genotyped by PCR using bPAC-specific primers (see also Figure 1A). Mice used in this study were 2–5 months of age. All animal experiments were in accordance with the relevant guidelines and regulations.

Slc9a10-null mice

Request a detailed protocol

Slc9a10-null mice were purchased from the Jackson Laboratory (B6;129S6-Slc9a10tm1Gar/J, stock number: 007661).

Western blot analysis

Request a detailed protocol

For heterologous expression, HEK293 cells were transfected using Lipofectamine 2000 (Life Technologies, Carlsbad, USA). Lysates were obtained by homogenizing cells or tissues in lysis buffer (10 mM Tris/HCl, pH 7.6, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, mPIC protease inhibitor cocktail 1:500) followed by trituration through a 18-gauge needle. Samples were incubated for 30 min on ice and centrifuged at 10,000×g for 5 min at 4°C. Prior to separation by SDS-PAGE, samples were mixed with 4× SDS loading buffer (200 mM Tric/HCl, pH 6.8, 8% SDS (wt/vol), 4% β-mercaptoethanol (vol/vol), 50% glycerol, 0.04% bromophenol blue) and heated for 5 min at 95°C. To activate bPAC, sperm samples were illuminated for 90 min in suspension in a reaction tube using a halogen fiber optics illuminator (Olympus, Tokio, Japan). Sperm samples used for SDS-PAGE were washed with 1 ml PBS and sedimented by centrifugation at 5000×g for 5 min. 1–2 × 106 cells were resuspended in 4× SDS loading buffer and heated for 5 min at 95°C. For Western blot analysis, proteins were transferred onto PVDF membranes (Merck Millipore, Billerica, USA), probed with antibodies, and analysed using a chemiluminescence detection system. For re-probing, membranes were incubated in stripping buffer (62.5 mM Tris/HCl pH 6.7, 2% SDS) for 30 min at 65°C and washed with PBS before incubation with a new antibody. For the study of protein phosphorylation, sperm were incubated in capacitating buffer containing 25 mM NaHCO3 for 10 min (PKA-dependent phosphorylation) or 90 min (tyrosine kinase-dependent phosphorylation). Similarly, bPAC sperm were illuminated for either 10 or 90 min using a halogen fibre optics illuminator (150 W, Intralux 6000-1; Volpi, Schlieren, Switzerland).

Primary antibodies: anti-HA 3F10 (1:5000; Roche, Basel, Switzerland), anti-SACY R21 (1:2000; CEP Biotech, Tamarac, USA), anti-phosphotyrosine 4G10 (1:1000; Merck Millipore), anti-PKA[C] 5B (1:4000; BD Transduction Laboratories, San Jose, USA), anti-phospho-PKA substrate (1:1000; Cell Signaling, Danvers, USA), anti-α-tubulin (1:5000; Sigma-Aldrich, Seelze, Germany); secondary antibodies: goat-anti-rat, HRP conjugated (1:5000, Dianova, Hamburg, Germany), sheep-anti-mouse, HRP conjugated (1:5000, Dianova).

Immunohistochemistry

Request a detailed protocol

Testes were fixed in 4% paraformaldehyde/PBS overnight, cryo-protected in 10 and 30% sucrose, and embedded in Tissue-Tek (Sakura Finetek, Alphen aan den Rijn, Netherlands). To block unspecific binding sites, cryosections (16 µm) were incubated for 1 hr with blocking buffer (0.5% Triton X-100 and 5% ChemiBLOCKER (Merck Millipore) in 0.1 M phosphate buffer, pH 7.4). The primary anti-HA antibody (rat monoclonal; Roche) was diluted 1:1000 in blocking buffer and incubated for 2 hr. Fluorescent secondary antibodies (donkey anti-rat Alexa488; Dianova) was diluted 1:400 in blocking buffer containing 0.5 mg/ml DAPI (Life Technologies) and pictures were taken on a confocal microscope (FV1000; Olympus).

Sperm preparation

Request a detailed protocol

Sperm were isolated by incision of the cauda followed by a swim-out in modified TYH medium (135 mM NaCl, 4.8 mM KCl, 2 mM CaCl2, 1.2 mM KH2PO4, 1 mM MgSO4, 5.6 mM glucose, 0.5 mM sodium pyruvate, 10 mM L-lactate, 10 mM HEPES, pH 7.4). For capacitation, the medium contained 3 mg/ml BSA and 25 mM of the NaCl was substituted with 25 mM NaHCO3. The pH was adjusted at 37°C. After 15–30 min swim-out at 37°C, sperm were collected and counted. Red light was used during the isolation of bPAC sperm.

Determination of cAMP content

Request a detailed protocol

Sperm were adjusted to a concentration of 1.25 × 107 cells per ml with TYH buffer containing different compounds with the following final concentrations: 25 mM NaHCO3, 1 mM N6,2′-O-dibutyryladenosine-3′,5′-cyclic monophosphate (db-cAMP; Sigma-Aldrich), 0.5 mM IBMX (Sigma-Aldrich). To activate bPAC, sperm samples were illuminated in suspension in a reaction tube using a halogen fiber-optics illuminator (150 W, Intralux 6000-1; Volpi, Schlieren, Switzerland). After stimulation with light or compounds for 1 to 10 min, the reaction was quenched with HClO4 (1:3 (vol/vol); 0.5 M final concentration). Samples were frozen in liquid N2, thawed, and neutralized by addition of K3PO4 (0.24 M final concentration). The salt precipitate and cell debris were sedimented by centrifugation (15 min, 15,000 g, 4°C). The cAMP content in the supernatant was determined by a competitive immunoassay (Molecular Devices, Sunnyvale, USA), including an acetylation step for higher sensitivity. Calibration curves were obtained by serial dilutions of cAMP standards. 96-well plates were analysed by using a microplate reader (FLUOstar Omega; BMGLabtech, Ortenberg, Germany).

Analysis of acrosomal exocytosis

Request a detailed protocol

1 × 106 sperm were capacitated in TYH buffer supplemented with 3 mg/ml BSA and 25 mM NaHCO3. Non-capacitated sperm were incubated in BSA buffer only. To induce capacitation by light, sperm were illuminated with a halogen fiber-optics illuminator (150 W, Intralux 6000-1; Volpi) at 37°C for 90 min. The acrosome reaction was induced by incubating sperm with 2 µM ionomycin for 10 min, incubation with 1% DMSO served as control. Sperm were collected by centrifugation and resuspended in 100 µl PBS buffer. Samples were air dried on microscope slides and fixed with 100% ethanol for 30 min at room temperature. For acrosome staining, sperm were labeled with 5 μg/ml PNA-FITC (L7381, Sigma-Aldrich) and 2 μg/ml DAPI in PBS for 30 min. Images were acquired using a confocal laser scanning microscope (FV1000; Olympus), a minimum of 600 cells was analyzed per condition.

Sperm motility analysis

Request a detailed protocol

Sperm motility was studied in shallow observation chambers (depth 150 µm). Cells were tethered to the glass surface by adjusting the BSA concentration in the buffer to 0.3 mg/ml. For analysis, cells that had their head attached to the glass surface and that had a free beating flagellum were chosen. Sperm motility was recorded under an inverted microscope (IX71; Olympus) equipped with a dark-field condenser, a 10x objective (UPlanFL, NA 0.3; Olympus), and additional 1.6× lenses (16× final magnification). The temperature of the microscope incubator (Life Imaging Services, Basel, Switzerland) was adjusted to 37°C. To obtain sharp images of moving sperm, stroboscopic illumination (2 ms light pulses) was achieved using a red LED (M660L3-C1; Thorlabs, Newton, USA) with a custom-made power supply. The camera and the LED light pulses were synchronized using a function generator (33220A; Agilent). Images were collected at 200 frames per second using a CMOS camera with a pixel size of 11 µm (Dimax; PCO, Kelheim, Germany). Stimulation of bPAC was achieved using a collimated UV LED (365 nm; ∼12 mW) coupled to the epifluorescence port of the microscope (M365L2-C1; Thorlabs) and a TTL-controlled custom-made power supply. Irradiation time was set using a TTL pulse generator (UTG100; ELV). For local activation of the flagellum of bPAC sperm, we partially focused the UV-LED onto a 100-µm diaphragm (Linos Photonik) by detuning its corresponding collimator lens. The diaphragm aperture was then imaged onto the objective focal plane by a plano-convex lens (f = 100 mm; LA1509A, Thorlabs). This resulted in a Gaussian-shaped UV spot (R2 of the fit 0.96) with a 12-µm width at the focal plane. The total UV-light power delivered was 2.3 mW. Quantification of the flagellar beat was performed using custom-made programs written in MATLAB (MathWorks. Natick, USA). The software can be made available upon request. The program identified the best threshold for binarization followed by a skeleton operation to identify the flagellum. The flagellar beat parameters were determined within a time window of 0.5 s before and after each frame. For frames at the boundaries (the beginning or the end of the video or flanking the UV flash), the time windows were asymmetric but contained the same number of frames. We monitored the angle between the straight line connecting the middle of the flagellum with the sperm head and the axis of symmetry of the cell. This angle varied in a sinusoid-like manner in time. The beat frequency was obtained by fitting a sinus to this wave. For alignment of the flagellar beat envelopes, we used custom-made programs written in LabVIEW. Using defined thresholds, the image was binarized. From a user-defined region-of-interest centred at the cell head, the program determined the location of the head on subsequent frames using a registering procedure. The neck of the cell was identified by applying a mask with the shape of an annulus centred into the sperm head. The annulus had an internal diameter of 16 µm to cover the sperm head and a 4 µm longer external diameter, enough to resolve the first pixels of the neck. All frames were then rotated and superimposed with a rotation angle equal to the azimuth of the neck region on a reference system centred at the sperm head. For better visualization, representative datasets were smoothed using Graph Pad Prism 5.02 (factor 20 for global illumination, factor 100 local illumination).

Measurement of changes in intracellular Ca2+

Request a detailed protocol

Changes in [Ca2+]i were recorded using the fluorescent Ca2+ indicators Cal-520, AM (AAT Bioquest, Sunnyvale, USA) and FluoForte (ENZO Life Sciences, Lörrach, Germany) in 96 multi-well plates in a fluorescence plate reader (Fluostar Omega; BMGLabtech) and in a rapid-mixing device in the stopped-flow mode (SFM400; Bio-Logic, Claix, France). For Ca2+ measurements in the plate reader, sperm were loaded with FluoForte (20 µM) in the presence of Pluronic F-127 (0.02% vol/vol) at 37°C for 45 min. After incubation, excess dye was removed by three centrifugation steps (700×g, 7 min, room temperature). The pellet was resuspended in TYH and equilibrated for 5 min at 37°C. Each well was filled with 100 µl (106 sperm ml−1) of the sperm suspension. To record changes in [Ca2+]i upon activation of bPAC, samples were alternately excited at 485 nm and 520 nm. As a control, fluorescence was recorded before and after the addition of 10 µl of different compounds to the final concentration of 10 mM (db-cAMP, 8-Bromo-cAMP, 8-Bromo-cGMP; Sigma-Aldrich). Changes in fluorescence are depicted as ΔF/F0 (%), indicating the percentage change in fluorescence (ΔF) with respect to the mean basal fluorescence (F0) before application of buffer or compounds.

To record changes in [Ca2+]i in a rapid-mixing device (SFM-400; Bio-Logic) in the stopped-flow mode, sperm were loaded with Cal-520 (5 μM) as described above for FluoForte. Changes in [Ca2+]i were measured as previously described (Strünker et al., 2011) with minor modifications. In brief, the sperm suspension (5 × 106 sperm/ml) was rapidly mixed 1:1 (vol/vol) with the respective stimulants at a flow rate of 0.5 ml/s. Fluorescence was excited by a SpectraX Light Engine (Lumencor, Beaverton, USA), whose intensity was modulated with a frequency of 10 kHz. The excitation light was passed through a BrightLine 475/28 nm filter (Semrock, Rochester, USA) onto the cuvette. Emission light was passed through a BrightLine 536/40 filter (Semrock) and recorded by photomultiplier modules (H10723-20; Hamamatsu Photonics). The signal was amplified and filtered through a lock-in amplifier (7230 DualPhase; Ametek, Paoli, USA). Data acquisition was performed with a data acquisition pad (PCI-6221; National Instruments. Austin, USA) and Bio-Kine software v. 4.49 (Bio-Logic). Ca2+ signals are depicted as the percent change in fluorescence (ΔF) with respect to the mean of the first three data points recorded immediately after mixing (F0), that is, when a stable fluorescence signal was observed. The control (buffer) ΔF/F0 signal was subtracted from compound-induced signals.

In vitro fertilization

Request a detailed protocol

Superovulation in females was induced by intraperitoneal injection of 10 I.U. Intergonan (SimposiumVet, Lisbon, Portugal) 3 days before the experiment. 14 hours before oocyte preparation, mice were injected with 10 I.U. Ovogest (SimposiumVet). KSOM medium (EmbryoMax Modified M16 Medium; Merck Millipore) was mixed 1:1 with mineral oil (Sigma-Aldrich) and equilibrated overnight at 37°C. On the day of preparation, 100 µl drops of KSOM medium were covered with the medium/oil mixture and 100,000 sperm were added to each drop. Sperm were capacitated for 90 min in TYH medium supplemented as indicated above. For stimulation of bPAC, the medium contained no HCO3− and reaction tubes containing sperm were placed in a custom-made rack equipped with blue LEDs for 90 min. Cumulus-enclosed oocytes were prepared from the oviducts of superovulated females and added to the drops. After 4 hr at 37°C and 5% CO2, oocytes were transferred to fresh KSOM medium. The number of 2-cell stages was evaluated after 24 hr.

References

    1. Austin CR
    (1952)
    The capacitation of the mammalian sperm
    Nature 170:326.
    1. Chang MC
    (1951)
    Fertilizing capacity of spermatozoa deposited into the fallopian tubes
    Nature 168:697–698.
    1. Rosenbaum JL
    2. Witman GB
    (2002) Intraflagellar transport
    Nature Reviews Molecular cell biology 3:813–825.
    https://doi.org/10.1038/nrm952
    1. Visconti PE
    2. Bailey JL
    3. Moore GD
    4. Pan D
    5. Olds-Clarke P
    6. Kopf GS
    (1995a)
    Capacitation of mouse spermatozoa. I. Correlation between the capacitation state and protein tyrosine phosphorylation
    Development 121:1129–1137.
    1. Visconti PE
    2. Moore GD
    3. Bailey JL
    4. Leclerc P
    5. Connors SA
    6. Pan D
    7. Olds-Clarke P
    8. Kopf GS
    (1995b)
    Capacitation of mouse spermatozoa. II. Protein tyrosine phosphorylation and capacitation are regulated by a cAMP-dependent pathway
    Development 121:1139–1150.

Decision letter

  1. David E Clapham
    Reviewing Editor; Howard Hughes Medical Institute, Boston Children's Hospital, United States

eLife posts the editorial decision letter and author response on a selection of the published articles (subject to the approval of the authors). An edited version of the letter sent to the authors after peer review is shown, indicating the substantive concerns or comments; minor concerns are not usually shown. Reviewers have the opportunity to discuss the decision before the letter is sent (see review process). Similarly, the author response typically shows only responses to the major concerns raised by the reviewers.

Thank you for sending your work entitled “Controlling fertilization and cAMP signaling in sperm flagella by optogenetics” for consideration at eLife. Your article has been favorably evaluated by a Senior editor, David Clapham as a guest Reviewing editor, and two peer reviewers.

The Reviewing editor and the reviewers discussed their comments before we reached this decision, and the Reviewing editor has assembled the following comments to help you prepare a revised submission.

The full reviewers comments are shown at the end of this letter, but the points for revision boil down to the following:

1) You need to more convincingly show that light stimulation in the imaging experiments induces a significant [cAMP] increase in the sperm principal piece.

2) You need to show that cAMP/cGMP analogs activate ICatSper in mouse sperm. You state: “However, these analogues failed to induce a Ca2+ influx in CatSper-null sperm (Figure 3F) (Ren et al, 2001), indicating that analogues of cyclic nucleotides, like in human sperm, nonspecifically activate also mouse CatSper. These results solve a long-standing controversy concerning the interconnection of cAMP and Ca2+ influx in mammalian sperm.” You assume that 8-Br-cAMP/cGMP also activates ICatSper in mouse sperm, “like in human sperm”. Although not referenced in this sentence, the authors are presumably referring to their previous paper (EMBOJ, 2012, 31,1654-1665, Figure 8) where high concentration (5 mM) 8-Br-cGMP enhanced a monovalent ICatSper-like current by ∼ 2 fold. Such a small change at this high dose is difficult to interpret. In the Kirichok et al. paper (Nature, 2006, 439: 737-740), 8-Br-cAMP was tested on mouse sperm, and there was no ICatSper activation. Thus, if you wish to support this statement, you could record mouse ICatSper changes with 8Br-cAMP and show that 8-Br-cAMP activation is nonspecific, perhaps by showing a dose response curve, comparing inside and outside perfusion of the analog, etc.

Alternatively, for both points, you could refrain from overextending your conclusions and simply state the points you have definitively shown. This would require removing the comments quoted above and alike comments in the discussion, and state without exaggeration the merits and current limitations of the experiments. This is an important new tool, and it is best to accurately state what is, and is not, possible to conclude. Further refinement of the indicator, or other indicators may enable you to definitively address these points in the future.

Reviewer #1:

In this manuscript, Jansen et al. made a transgenic mouse line expressing a bacterial photoactivated adenylyl cyclase (bPAC) in testis and sperm. bPAC was previously developed and tested by the Hegemann group in Xenopus oocytes and cultured neurons; this paper reports the first use of bPAC in whole animals. Light stimulation in the transgenic sperm is able to increase cAMP concentration, leads to the acrosome reaction and triggers sperm tail beating. In addition, light stimulation also rescues the fertililization deficiency in the sNHE (a sperm-specific putative Na+/H+ exchanger) knockout sperm.

The tool developed in the paper allowed the authors to directly test the function of cAMP. Previous experiments relied on the addition of bicarbonate, exogenously applied cell-permeable cAMP or cAMP UV-uncaging, which can be somewhat indirect. The results from the paper largely confirmed previous findings: cAMP is important for all the major sperm physiology including capacitation, motility and fertilization. One apparently surprising finding is that light stimulation does not lead to obvious increase of intracellular Ca2+ concentration.

The experiments in the paper are well designed and the data is convincing. This is the first reported optogenetic tool applied to sperm physiology. The tool will allow cAMP signaling studies with high temporal and spatial resolution in the future.

The only major comment I have is on the conclusion that cAMP does not induce Ca2+ influx ([Ca2+]i increase) in sperm, which is apparently at odd with various previous studies with “indirect” methods. While the finding in Figure 3B and C, that light stimulation does not lead to detectable change in Δ F/F, is consistent with the idea that cAMP doesn't cause Ca2+ influx, there can be other interpretations to the data. For example, the light-induced [cAMP] increase is relatively moderate (∼2 to 4 fold) and is comparable to that induced by HCO3 (Figure 2A). Earlier studies by Babcock and colleagues (e.g. Wennemuth, JBC, 2000) showed that large detectable HCO3- induced [Ca2+] increases were obvious only when combined with K8.6 stimulus (higher [K+] with alkali bath). It is possible that at the level achieved by light stimulation in the current study, cAMP requires other co-stimuli to induce Ca2+ influx. Another possibility, perhaps even more likely, is that the 2-4 fold [cAMP] increase, which is measured from whole, is largely from sperm head (because of its overwhelmingly larger volume) but little from sperm principal piece where the Ca2+ influx channels are localized. The experiments in the paper do not show whether there is [cAMP] increase in sperm tail upon light stimulation. The geometry of the sperm midpiece and the filled mitochondria perhaps are significant barriers for cAMP diffusion from sperm head to tail.

Reviewer #2:

This paper describes a novel animal model featuring expression of the light-activated adenylyl cyclase bPAC exclusively in sperm. The authors present exciting data showing rapid changes in flagellar beat in live sperm upon stimulation of bPAC, and that light can serve as a surrogate for other stimuli believed to elevate cAMP in sperm such as HCO3- treatment. The changes in flagellar beat also provide a useful (albeit indirect) readout of the temporal features of illumination-dependent cAMP production by bPAC.

This optogenetic tool will provide a fantastic opportunity to clarify the role of compartmentalized cAMP signaling in sperm motility, maturation and fertilization. The animal model is well-validated (Figure 1), and bPAC expression does not alter expression of other cAMP signaling proteins and pathways (Figure 2). While expression of bPAC did not alter PKA phosphorylation per se (Figure 2D), it would have been nice to see that illumination could also produce sufficient cAMP to stimulate phosphorylation of PKA targets. If the authors could produce such a figure, it would strengthen the paper.

In Figure 3, the authors confirm previous findings that cyclic nucleotide analogs (10 mM 8Br-cAMP and 10mM db-cAMP) caused Ca2+ entry into the sperm via CatSper, but light stimulation of bPAC did not cause this Ca2+ entry. Is another possible interpretation of this result simply that the illumination of bPAC didn't cause sufficient intracellular cAMP production to influence CatSper? On the other hand, the concentration of cAMP analogs in the present study is much higher than would typically be used in most other cell types to activate cAMP targets. Is this concentration comparable to what was used previously in other studies that examined the effects of cAMP analogs on calcium entry? What was the concentration of db-cAMP used in Figure 2E (cAMP-dependent tyrosine phosphorylation)?

https://doi.org/10.7554/eLife.05161.013

Author response

1) You need to more convincingly show that light stimulation in the imaging experiments induces a significant [cAMP] increase in the sperm principal piece.

We would love to do this experiment. Such an experiment requires the transgenic expression of a cAMP FRET biosensor in the flagellum of intact sperm; the spectral properties of the cAMP sensor and bPAC must be orthogonal. Such a sensor is not yet available. Notwithstanding, we show in a new data set (Figure 2K and Movie 4, 5) that stimulation of a small part of the flagellum with a single flash increased the flagellar beat frequency, demonstrating that local bPAC activation significantly increases cAMP levels in the flagellum. We have amended the Results, Discussion, and Materials and methods sections accordingly and added a sentence to the discussion stating that it would be ideal to combine optogenetic tools with a fluorescent cAMP biosensor to study cAMP dynamics in live cells.

2) You need to show that cAMP/cGMP analogs activate ICatSper in mouse sperm. You state: “However, these analogues failed to induce a Ca2+ influx in CatSper-null sperm (Figure 3F) (Ren et al, 2001), indicating that analogues of cyclic nucleotides, like in human sperm, nonspecifically activate also mouse CatSper. These results solve a long-standing controversy concerning the interconnection of cAMP and Ca2+ influx in mammalian sperm.” You assume that 8-Br-cAMP/cGMP also activates ICatSper in mouse sperm, “like in human sperm”. Although not referenced in this sentence, the authors are presumably referring to their previous paper (EMBOJ, 2012, 31,1654-1665, Figure 8) where high concentration (5 mM) 8-Br-cGMP enhanced a monovalent ICatSper-like current by ∼ 2 fold. Such a small change at this high dose is difficult to interpret. In the Kirichok et al. paper (Nature, 2006, 439: 737-740), 8-Br-cAMP was tested on mouse sperm, and there was no ICatSper activation. Thus, if you wish to support this statement, you could record mouse ICatSper changes with 8Br-cAMP and show that 8-Br-cAMP activation is nonspecific, perhaps by showing a dose response curve, comparing inside and outside perfusion of the analog, etc.

Alternatively, for both points, you could refrain from overextending your conclusions and simply state the points you have definitively shown. This would require removing the comments quoted above and alike comments in the discussion, and state without exaggeration the merits and current limitations of the experiments. This is an important new tool, and it is best to accurately state what is, and is not, possible to conclude. Further refinement of the indicator, or other indicators may enable you to definitively address these points in the future.

Millimolar concentrations (1-10 mM) of 8-Br-cGMP and -cAMP are commonly used to stimulate Ca2+ entry in mammalian sperm (e.g. Ren et al., 2001, Nature; Xia et al., 2007; Biology of Reproduction, Fukuda et al., 2004, Journal of Cell Science). Genetic ablation of CatSper in mice abolishes 8-Br-cNMP-evoked Ca2+ entry (Ren et al., 2001, Nature), suggesting that these compounds act via CatSper. In Brenker et al. 2012 (EMBO Journal), we reveal that 8-Br-cGMP in fact directly activates human CatSper, rather than a “CatSper-like” channel, via an extracellular site; the current increases by 100%, which we consider a significant change.

The results in human sperm suggest that these chemicals might also directly activate mouse CatSper. In fact, studies on the action of 8-Br-cNMPs on membrane currents in mouse sperm yielded mixed results: Kirichok et al., 2006 (Nature) did not observe an action of 8-Br-cNMPs (at 5 mM), whereas Cisneros-Mejorado et al. (2014, Andrology, and 2012, FEBS Letters) show that 8-Br-cNMPs (0.2 – 0.8 mM) enhance monovalent currents; these 8-Br-cNMP-induced currents are presumably mediated by CatSper rather than by CNG channels as the authors concluded. We did not scrutinize the direct action of cNMP analogs on mouse sperm and we don’t want to make any unwarranted claims. Therefore, we followed the referee´s suggestion and rephrased the paragraph. We now state that: “in contrast, in wild-type and bPAC sperm, 8-Bromo-cAMP, 8-Bromo-cGMP, and db-cAMP evoked a Ca2+ signal (Figure 3A–C, E) that is abolished in CatSper-null sperm (Figure 3F), similar to what has been reported by others (Ren et al, 2001; Xia and Ren, 2007)”.

Reviewer #1:

The only major comment I have is on the conclusion that cAMP does not induce Ca2+ influx ([Ca2+]i increase) in sperm, which is apparently at odd with various previous studies with “indirect” methods. While the finding in Figure 3B and C, that light stimulation does not lead to detectable change in Δ F/F, is consistent with the idea that cAMP doesn't cause Ca2+ influx, there can be other interpretations to the data. For example, the light-induced [cAMP] increase is relatively moderate (∼2 to 4 fold) and is comparable to that induced by HCO3 (Figure 2A). Earlier studies by Babcock and colleagues (e.g. Wennemuth, JBC, 2000) showed that large detectable HCO3- induced [Ca2+] increases were obvious only when combined with K8.6 stimulus (higher [K+] with alkali bath). It is possible that at the level achieved by light stimulation in the current study, cAMP requires other co-stimuli to induce Ca2+ influx. Another possibility, perhaps even more likely, is that the 2-4 fold [cAMP] increase, which is measured from whole, is largely from sperm head (because of its overwhelmingly larger volume) but little from sperm principal piece where the Ca2+ influx channels are localized. The experiments in the paper do not show whether there is [cAMP] increase in sperm tail upon light stimulation. The geometry of the sperm midpiece and the filled mitochondria perhaps are significant barriers for cAMP diffusion from sperm head to tail.

Previously, two different experimental protocols had been applied that prompted different interpretations. First, in mice sperm, a cAMP increase alone upon stimulation with HCO3- does not stimulate a Ca2+ influx (e.g. Wennemuth et al., 2000, Journal of Biological Chemistry; Wennemuth et al., 2003, Development; Carlson et al., 2007, Developmental Biology). These previous results by Dr. Babcock´s group completely agree with our results. However, if Ca2+ influx was provoked by high K+/alkaline pH (conditions that activate CatSper), HCO3- increases and accelerates the amplitude and kinetics, respectively, of the K8.6-stimulated Ca2+ increases (e.g. Wennemuth et al. 2003, Development). Thus, cAMP might sensitize CatSper to open upon depolarization and alkalization, but cAMP does not open CatSper on its own. Concerning the light-induced cAMP increase in the flagellum, please see our response to the Reviewing Editor.

Reviewer #2:

This paper describes a novel animal model featuring expression of the light-activated adenylyl cyclase bPAC exclusively in sperm. The authors present exciting data showing rapid changes in flagellar beat in live sperm upon stimulation of bPAC, and that light can serve as a surrogate for other stimuli believed to elevate cAMP in sperm such as HCO3- treatment. The changes in flagellar beat also provide a useful (albeit indirect) readout of the temporal features of illumination-dependent cAMP production by bPAC.

This optogenetic tool will provide a fantastic opportunity to clarify the role of compartmentalized cAMP signaling in sperm motility, maturation and fertilization. The animal model is well-validated (Figure 1), and bPAC expression does not alter expression of other cAMP signaling proteins and pathways (Figure 2). While expression of bPAC did not alter PKA phosphorylation per se (Figure 2D), it would have been nice to see that illumination could also produce sufficient cAMP to stimulate phosphorylation of PKA targets. If the authors could produce such a figure, it would strengthen the paper.

We performed a full new data set and replaced Figure 2D with a new figure showing both bicarbonate- and light-induced phosphorylation of PKA targets. We have amended the Materials and methods section accordingly.

In Figure 3, the authors confirm previous findings that cyclic nucleotide analogs (10 mM 8Br-cAMP and 10mM db-cAMP) caused Ca2+ entry into the sperm via CatSper, but light stimulation of bPAC did not cause this Ca2+ entry. Is another possible interpretation of this result simply that the illumination of bPAC didn't cause sufficient intracellular cAMP production to influence CatSper? On the other hand, the concentration of cAMP analogs in the present study is much higher than would typically be used in most other cell types to activate cAMP targets. Is this concentration comparable to what was used previously in other studies that examined the effects of cAMP analogs on calcium entry? What was the concentration of db-cAMP used in Figure 2E (cAMP-dependent tyrosine phosphorylation)?

Concerning the use and action of 8-Br-cNMPs, please see our response to the Reviewing Editor. A db-cAMP-induced Ca2+ influx via CatSper has not been reported before. In general, this drug is used at millimolar concentrations to interfere with cAMP signaling. The concentration of db-cAMP used in Figure 2E was 1 mM, similar to what has been used in other studies (Visconti et al., 1955, Development). We have included this information in the figure legend.

https://doi.org/10.7554/eLife.05161.014

Article and author information

Author details

  1. Vera Jansen

    1. Department of Molecular Sensory Systems, Center of Advanced European Studies and Research, Bonn, Germany
    2. Minerva Research Group Molecular Physiology, Center of Advanced European Studies and Research, Bonn, Germany
    Contribution
    VJ, Conception and design, Acquisition of data, Analysis and interpretation of data, Drafting or revising the article
    Competing interests
    The authors declare that no competing interests exist.

  2. Luis Alvarez

    Department of Molecular Sensory Systems, Center of Advanced European Studies and Research, Bonn, Germany
    Contribution
    LA, Conception and design, Analysis and interpretation of data, Contributed unpublished essential data or reagents
    Competing interests
    The authors declare that no competing interests exist.

  3. Melanie Balbach

    Department of Molecular Sensory Systems, Center of Advanced European Studies and Research, Bonn, Germany
    Contribution
    MB, Acquisition of data, Analysis and interpretation of data
    Competing interests
    The authors declare that no competing interests exist.

  4. Timo Strünker

    Department of Molecular Sensory Systems, Center of Advanced European Studies and Research, Bonn, Germany
    Contribution
    TS, Conception and design, Analysis and interpretation of data, Drafting or revising the article
    Competing interests
    The authors declare that no competing interests exist.

  5. Peter Hegemann

    Institute of Biology, Experimental Biophysics, Humboldt University of Berlin, Berlin, Germany
    Contribution
    PH, Drafting or revising the article, Contributed unpublished essential data or reagents
    Competing interests
    The authors declare that no competing interests exist.

  6. U Benjamin Kaupp

    Department of Molecular Sensory Systems, Center of Advanced European Studies and Research, Bonn, Germany
    Contribution
    UBK, Conception and design, Drafting or revising the article
    For correspondence
    U.B.Kaupp@caesar.de
    Competing interests
    The authors declare that no competing interests exist.

  7. Dagmar Wachten

    Minerva Research Group Molecular Physiology, Center of Advanced European Studies and Research, Bonn, Germany
    Contribution
    DW, Conception and design, Analysis and interpretation of data, Drafting or revising the article
    For correspondence
    dagmar.wachten@caesar.de
    Competing interests
    The authors declare that no competing interests exist.

Funding

Max-Planck-Gesellschaft (Minerva Program)

  • Dagmar Wachten

University of Bonn (Maria-von-Linden Program)

  • Dagmar Wachten

Deutsche Forschungsgemeinschaft (SFB645 (INST 217/555-2))

  • Timo Strünker
  • U Benjamin Kaupp
  • Dagmar Wachten

Deutsche Forschungsgemeinschaft (Bonn Excellence Cluster ImmunoSensation)

  • Luis Alvarez
  • U Benjamin Kaupp
  • Dagmar Wachten

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Acknowledgements

We thank N Blank, JH Krause, R Pascal, and I Lux for technical assistance, W Bönigk for cloning, and R Braun (Jackson Laboratory) for providing the pPrCExV vector.

Ethics

Animal experimentation: This study was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the LANUV (Landesamt für Natur, Umwelt und Verbraucherschutz). Reference number: 84-02.04.2012.A192.

Reviewing Editor

  1. David E Clapham, Howard Hughes Medical Institute, Boston Children's Hospital, United States

Version history

  1. Received: October 14, 2014
  2. Accepted: December 22, 2014
  3. Version of Record published: January 20, 2015 (version 1)

Copyright

© 2015, Jansen et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 5,056
    Page views
  • 684
    Downloads
  • 85
    Citations

Article citation count generated by polling the highest count across the following sources: Scopus, Crossref, PubMed Central.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Open citations (links to open the citations from this article in various online reference manager services)

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

  1. Vera Jansen
  2. Luis Alvarez
  3. Melanie Balbach
  4. Timo Strünker
  5. Peter Hegemann
  6. U Benjamin Kaupp
  7. Dagmar Wachten
(2015)
Controlling fertilization and cAMP signaling in sperm by optogenetics
eLife 4:e05161.
https://doi.org/10.7554/eLife.05161

Categories and tags

Research organism

Further reading

  1. Remote controlled sperm: Listen to Vera Jansen and Dagmar Wachten talk about using optogenetics to control sperm

    Podcast

    Light can be used to control the motility of sperm.

    1. Cell Biology
    2. Medicine

    Glucagon-like peptide-1 receptor activation stimulates PKA-mediated phosphorylation of Raptor and this contributes to the weight loss effect of liraglutide

    Thao DV Le, Dianxin Liu ... Julio E Ayala
    Research Article Updated

    The canonical target of the glucagon-like peptide-1 receptor (GLP-1R), Protein Kinase A (PKA), has been shown to stimulate mechanistic Target of Rapamycin Complex 1 (mTORC1) by phosphorylating the mTOR-regulating protein Raptor at Ser791 following β-adrenergic stimulation. The objective of these studies is to test whether GLP-1R agonists similarly stimulate mTORC1 via PKA phosphorylation of Raptor at Ser791 and whether this contributes to the weight loss effect of the therapeutic GLP-1R agonist liraglutide. We measured phosphorylation of the mTORC1 signaling target ribosomal protein S6 in Chinese Hamster Ovary cells expressing GLP-1R (CHO-Glp1r) treated with liraglutide in combination with PKA inhibitors. We also assessed liraglutide-mediated phosphorylation of the PKA substrate RRXS*/T* motif in CHO-Glp1r cells expressing Myc-tagged wild-type (WT) Raptor or a PKA-resistant (Ser791Ala) Raptor mutant. Finally, we measured the body weight response to liraglutide in WT mice and mice with a targeted knock-in of PKA-resistant Ser791Ala Raptor. Liraglutide increased phosphorylation of S6 and the PKA motif in WT Raptor in a PKA-dependent manner but failed to stimulate phosphorylation of the PKA motif in Ser791Ala Raptor in CHO-Glp1r cells. Lean Ser791Ala Raptor knock-in mice were resistant to liraglutide-induced weight loss but not setmelanotide-induced (melanocortin-4 receptor-dependent) weight loss. Diet-induced obese Ser791Ala Raptor knock-in mice were not resistant to liraglutide-induced weight loss; however, there was weight-dependent variation such that there was a tendency for obese Ser791Ala Raptor knock-in mice of lower relative body weight to be resistant to liraglutide-induced weight loss compared to weight-matched controls. Together, these findings suggest that PKA-mediated phosphorylation of Raptor at Ser791 contributes to liraglutide-induced weight loss.

    1. Cell Biology
    2. Developmental Biology

    Cylicins are a structural component of the sperm calyx being indispensable for male fertility in mice and human

    Simon Schneider, Andjela Kovacevic ... Hubert Schorle
    Research Article

    Cylicins are testis-specific proteins, which are exclusively expressed during spermiogenesis. In mice and humans, two Cylicins, the gonosomal X-linked Cylicin 1 (Cylc1/CYLC1) and the autosomal Cylicin 2 (Cylc2/CYLC2) genes, have been identified. Cylicins are cytoskeletal proteins with an overall positive charge due to lysine-rich repeats. While Cylicins have been localized in the acrosomal region of round spermatids, they resemble a major component of the calyx within the perinuclear theca at the posterior part of mature sperm nuclei. However, the role of Cylicins during spermiogenesis has not yet been investigated. Here, we applied CRISPR/Cas9-mediated gene editing in zygotes to establish Cylc1- and Cylc2-deficient mouse lines as a model to study the function of these proteins. Cylc1 deficiency resulted in male subfertility, whereas Cylc2-/-, Cylc1-/yCylc2+/-, and Cylc1-/yCylc2-/- males were infertile. Phenotypical characterization revealed that loss of Cylicins prevents proper calyx assembly during spermiogenesis. This results in decreased epididymal sperm counts, impaired shedding of excess cytoplasm, and severe structural malformations, ultimately resulting in impaired sperm motility. Furthermore, exome sequencing identified an infertile man with a hemizygous variant in CYLC1 and a heterozygous variant in CYLC2, displaying morphological abnormalities of the sperm including the absence of the acrosome. Thus, our study highlights the relevance and importance of Cylicins for spermiogenic remodeling and male fertility in human and mouse, and provides the basis for further studies on unraveling the complex molecular interactions between perinuclear theca proteins required during spermiogenesis.