Auxin regulates SNARE-dependent vacuolar morphology restricting cell size

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Cite this article as: eLife 2015;4:e05868 doi: 10.7554/eLife.05868

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Abstract

The control of cellular growth is central to multicellular patterning. In plants, the encapsulating cell wall literally binds neighbouring cells to each other and limits cellular sliding/migration. In contrast to its developmental importance, growth regulation is poorly understood in plants. Here we reveal that the phytohormone auxin impacts on the shape of the biggest plant organelle, the vacuole. TIR1/AFBs-dependent auxin signalling posttranslationally controls the protein abundance of vacuolar SNARE components. Genetic and pharmacological interference with the auxin effect on vacuolar SNAREs interrelates with auxin-resistant vacuolar morphogenesis and cell size regulation. Vacuolar SNARE VTI11 is strictly required for auxin-reliant vacuolar morphogenesis and loss of function renders cells largely insensitive to auxin-dependent growth inhibition. Our data suggests that the adaptation of SNARE-dependent vacuolar morphogenesis allows auxin to limit cellular expansion, contributing to root organ growth rates.

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Author details

Christian Löfke

Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna, Austria

Competing interests
The authors declare that no competing interests exist.
Kai Dünser

Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna, Austria

Competing interests
The authors declare that no competing interests exist.
David Scheuring

Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna, Austria

Competing interests
The authors declare that no competing interests exist.
Jürgen Kleine-Vehn

Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna, Austria

For correspondence
juergen.kleine-vehn@boku.ac.at

Competing interests
The authors declare that no competing interests exist.

Reviewing Editor

Christian S Hardtke, University of Lausanne, Switzerland

Publication history

Received: December 3, 2014
Accepted: March 5, 2015
Accepted Manuscript published: March 5, 2015 (version 1)
Version of Record published: April 2, 2015 (version 2)

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This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.