Regions within a single epidermal cell of Drosophila can be planar polarised independently

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Abstract

Planar cell polarity (PCP), the coordinated and consistent orientation of cells in the plane of epithelial sheets, is a fundamental and conserved property of animals and plants. Up to now, the smallest unit expressing PCP has been considered to be an entire single cell. We report that, in the larval epidermis of Drosophila, different subdomains of one cell can have opposite polarities. In larvae, PCP is driven by the Dachsous/Fat system; we show that the polarity of a subdomain within one cell is its response to levels of Dachsous/Fat in the membranes of contacting cells. During larval development, cells rearrange (Saavedra et al 2014), and when two subdomains of a single cell have different types of neighbouring cells, then these subdomains can become polarised in opposite directions. We conclude that polarisation depends on a local comparison of the amounts of Dachsous and Fat within opposing regions of a cell's membrane.

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Miguel Rovira

Department of Zoology, University of Cambridge, Cambridge, United Kingdom

Competing interests
The authors declare that no competing interests exist.
Pedro Saavedra

Institute of Molecular and Cell Biology, Proteos, Singapore

Competing interests
The authors declare that no competing interests exist.
José Casal

Department of Zoology, University of Cambridge, Cambridge, United Kingdom

For correspondence
jec85@cam.ac.uk

Competing interests
The authors declare that no competing interests exist.
Peter A Lawrence

Department of Zoology, University of Cambridge, Cambridge, United Kingdom

Competing interests
The authors declare that no competing interests exist.

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