(A) Locations of sequenced mycobacteriophages across the globe. (B) Locations of sequenced mycobacteriophages across the United States. Colors and letter designations on the isolates refer to the …
Complete genome sequences of 627 mycobacteriophages were concatenated into a single file which was compared with itself using Gepard (Krumsiek et al., 2007) and displayed as a dotplot using default …
Concatenated DNA sequences for 627 phage genomes.
A dotplot was generated using a concatenated file of genome sequences using Gepard (Krumsiek et al., 2007). The complexity of the genome relationships is illustrated by the Cluster I phages which …
The dotplot of concatenated genome sequences illustrates the ambiguity of whether the singleton Dori warrants inclusion in Cluster B. Dori shares DNA sequence similarity with its closest relative …
The dotplot of concatenated genome sequences illustrates the complex relationships between the singleton MooMoo and other phages. MooMoo shares DNA sequence similarity with SG4 (Subcluster F1) and …
Genomes of 627 mycobacteriophages were compared according to shared gene content using the Phamerator (Cresawn et al., 2011) database Mykobacteriophage_627, and displayed using SplitsTree (Huson and …
Nexus file containing phamily assignments for 627 phage genomes.
The proportions of genes that are orphams (i.e., single-gene phamilies with no homologues within the mycobacteriophage dataset) are shown for each phage. The order of the phages is as shown in Supple…
Pham table containing phamily designations for 627 phage genomes.
(A) Average percentages of phamilies shared between Dori and other mycobacteriophages. (B) Average percentages of phamilies shared between MooMoo and other mycobacteriophages. Genomes on the x axis …
(A) Average percentages of phamilies shared between Gaia and other mycobacteriophages. (B) Average percentages of phamilies shared between Sparky and other mycobacteriophages. Genomes on the x axis …
The percentages of pairwise shared genes was determined using a Phamerator (Cresawn et al., 2011) database (Mykobacteriophage_627) populated with 627 completely sequenced phage genomes. The 69,574 …
Dataset showing percentages of pairwise shared phamilies.
(A) The CLuster Averaged Shared Phamilies (CLASP; blue), Cluster Associated Phamilies (CAP; red) and Cluster Cohesion Index (CCI; green) values are plotted for each mycobacteriophage cluster. (B) …
Datasets showing numbers of CLuster Average Shard Phamilies.
CLuster Averaged Shared Phamilies (CLASP) values were calculated for Clusters A, B, C, E, F, and K by resampling random subsets of the genomes. The size of the subsets is shown on the x axis and …
The CAP and CPV values are plotted for each cluster.
(A) The numbers of phamilies are reported for between 1 and 627 phage genomes sampled at random without replacement; the mean of 10,000 iterations is shown in red; gray lines indicate a confidence …
Datasets for determination of rarefaction curves.
Diversity and genetic isolation of mycobacteriophage genome clusters
Cluster | # Subclusters | # Genomes | Average # genes* | Average length (bp) | Total phams† | Total genes | CLASP‡ | CAP§ | CCI# | CII¶ |
---|---|---|---|---|---|---|---|---|---|---|
A | 11 | 232 | 90 ± 5.3 | 51,514 | 1085 | 20,880 | 38.3 | 12.4 | 0.08 | 80.2 |
B | 5 | 109 | 100.4 ± 4.5 | 68,653 | 421 | 10,944 | 66.2 | 23.2 | 0.24 | 81.0 |
C | 2 | 45 | 231 ± 5.9 | 155,504 | 486 | 10,395 | 89.3 | 29.4 | 0.48 | 84.6 |
D | 2 | 10 | 89.3 ± 6.4 | 64,965 | 147 | 893 | 88.1 | 64.3 | 0.61 | 71.4 |
E | 1 | 35 | 141.9 ± 3.4 | 75,526 | 236 | 4967 | 87.2 | 63.8 | 0.60 | 59.3 |
F | 3 | 66 | 105.3 ± 5.3 | 57,416 | 658 | 6950 | 54.4 | 4.9 | 0.16 | 55.8 |
G | 1 | 14 | 61.5 ± 1.2 | 41,845 | 72 | 861 | 96.0 | 91.1 | 0.85 | 55.6 |
H | 2 | 5 | 98.4 ± 5.7 | 69,469 | 207 | 492 | 61.6 | 31.5 | 0.48 | 67.6 |
I | 2 | 4 | 78 ± 3.7 | 49,954 | 147 | 312 | 58.9 | 35.0 | 0.53 | 23.8 |
J | 1 | 16 | 239.8 ± 9.3 | 110,332 | 530 | 3776 | 70.8 | 40.1 | 0.45 | 58.5 |
K | 5 | 32 | 95.7 ± 4.6 | 59,720 | 411 | 3069 | 51.8 | 20.0 | 0.23 | 73.5 |
L | 3 | 13 | 127.9 ± 6.5 | 75,177 | 246 | 1663 | 78.2 | 50.8 | 0.52 | 72.4 |
M | 2 | 3 | 141 ± 8.8 | 81,636 | 201 | 423 | 73.5 | 63.0 | 0.70 | 69.2 |
N | 1 | 7 | 69.1 ± 2.2 | 42,888 | 152 | 484 | 64.1 | 45.6 | 0.45 | 40.8 |
O | 1 | 5 | 124.2 ± 3.1 | 70,651 | 151 | 621 | 90.6 | 83.3 | 0.82 | 64.2 |
P | 2 | 9 | 78.8 ± 2.1 | 47,668 | 159 | 709 | 76.1 | 42.3 | 0.50 | 34.0 |
Q | 1 | 5 | 85.2 ± 3.7 | 53,755 | 90 | 426 | 96.6 | 90.4 | 0.95 | 73.3 |
R | 1 | 4 | 101.5 ± 2.5 | 71,348 | 117 | 406 | 91.4 | 84.8 | 0.87 | 71.8 |
S | 1 | 2 | 109 ± 2.0 | 65,172 | 117 | 218 | 91.7 | 91.7 | 0.93 | 70.9 |
T | 1 | 3 | 66.7 ± 2.4 | 42,833 | 83 | 200 | 86.1 | 82.5 | 0.80 | 62.7 |
Dori | 1 | 1 | 94 | 64,613 | 94 | 94 | N/A | N/A | N/A | 35.8 |
DS6A | 1 | 1 | 97 | 60,588 | 96 | 97 | N/A | N/A | N/A | 58.3 |
Gaia | 1 | 1 | 194 | 90,460 | 193 | 194 | N/A | N/A | N/A | 58.0 |
MooMoo | 1 | 1 | 98 | 55,178 | 98 | 98 | N/A | N/A | N/A | 31.6 |
Muddy | 1 | 1 | 71 | 48,228 | 70 | 71 | N/A | N/A | N/A | 71.4 |
Patience | 1 | 1 | 109 | 70,506 | 109 | 109 | N/A | N/A | N/A | 57.8 |
Sparky | 1 | 1 | 93 | 63,334 | 93 | 93 | N/A | N/A | N/A | 48.4 |
Wildcat | 1 | 1 | 148 | 78,296 | 148 | 148 | N/A | N/A | N/A | 69.6 |
Average number of protein-coding genes per genome, with standard deviation.
Total phams is the sum of all phamilies (groups of homologous mycobacteriophage genes) in that cluster.
The Cluster Averaged Shared Phamilies (CLASP) index is the average of the percentages of phamilies shared pairwise between genomes within a cluster.
The Cluster-Associated Phamilies (CAP) index is the percentage of the average number of phamilies per genome within a cluster whose phamilies are present in every cluster member.
The Cluster Cohesion Index (CCI) is generated by dividing the average number of genes per genome by the total number of phamilies (phams) in that cluster.
The Cluster Isolation Index (CII) is the percentage of phams that are present only in that cluster, and not present in other mycobacteriophages.
N/A: Not applicable.
Genometrics and Cluster Cohesion Indexes of mycobacteriophages
Cluster | Subcluster | # Genomes | Average # genes | Average length (bp) | # Phams | CLASP* | CAP† | CCI‡ |
---|---|---|---|---|---|---|---|---|
A | 232 | 90.0 | 51,514 | 1085 | 38.3 | 12.4 | 8.0 | |
A1 | 72 | 91.2 | 51,954 | 416 | 72.3 | 36.9 | 22.0 | |
A2 | 28 | 93.4 | 52,805 | 312 | 64.7 | 30.1 | 30.0 | |
A3 | 37 | 87.7 | 50,325 | 163 | 81.1 | 48.8 | 54.0 | |
A4 | 46 | 87.4 | 51,376 | 125 | 92.7 | 70.6 | 70.0 | |
A5 | 16 | 86.0 | 50,531 | 152 | 81.4 | 58.7 | 57.0 | |
A6 | 11 | 97.8 | 51,677 | 128 | 90.2 | 75.1 | 76.0 | |
A7 | 3 | 84.3 | 52,941 | 115 | 74.9 | 64.4 | 73.0 | |
A8 | 4 | 97.8 | 51,597 | 107 | 93.5 | 86.8 | 91.0 | |
A9 | 4 | 96.0 | 52,838 | 106 | 92.7 | 83.4 | 91.0 | |
A10 | 7 | 80.0 | 49,174 | 112 | 81.6 | 60.9 | 71.0 | |
A11 | 4 | 98.5 | 52,260 | 113 | 93.6 | 88.3 | 87.0 | |
B | 108 | 100.4 | 68,653 | 421 | 66.2 | 23.2 | 24.0 | |
B1 | 77 | 101.8 | 68,532 | 144 | 93.2 | 72.9 | 71.0 | |
B2 | 8 | 89.9 | 67,267 | 101 | 94.9 | 84.6 | 89.0 | |
B3 | 12 | 102.8 | 68,698 | 121 | 96.3 | 84.7 | 85.0 | |
B4 | 8 | 96.1 | 70,619 | 166 | 79.9 | 45.8 | 58.0 | |
B5 | 3 | 96.3 | 70,033 | 108 | 91.7 | 87.2 | 89.0 | |
C | 45 | 231.0 | 155,504 | 486 | 89.3 | 29.4 | 48.0 | |
C1 | 44 | 231.0 | 155,297 | 345 | 91.9 | 73.2 | 67.0 | |
C2 | 1 | 229.0 | 164,602 | 227 | N/A | N/A | N/A | |
D | 10 | 89.3 | 64,965 | 147 | 88.1 | 64.3 | 61.0 | |
D1 | 9 | 87.3 | 64,697 | 100 | 94.9 | 88.8 | 87.0 | |
D2 | 1 | 107.0 | 67,383 | 107 | N/A | N/A | N/A | |
E | 35 | 141.9 | 75,526 | 235 | 87.2 | 63.8 | 60.0 | |
F | 66 | 105.3 | 57,416 | 658 | 54.4 | 4.9 | 16.0 | |
F1 | 60 | 104.8 | 57,486 | 573 | 59.6 | 20.6 | 18.0 | |
F2 | 5 | 110.8 | 55,996 | 207 | 65.7 | 49.0 | 54.0 | |
F3 | 1 | 107.0 | 60,285 | 105 | N/A | N/A | N/A | |
G | 14 | 61.5 | 41,845 | 72 | 96.0 | 91.1 | 85.0 | |
H | 5 | 98.4 | 69,469 | 207 | 61.6 | 31.5 | 48.0 | |
H1 | 4 | 95.8 | 69,137 | 131 | 81.9 | 67.9 | 73.0 | |
H2 | 1 | 109.0 | 70,797 | 110 | N/A | N/A | N/A | |
I | 4 | 78.0 | 49,954 | 147 | 58.9 | 35.0 | 53.0 | |
I1 | 3 | 76.0 | 47,588 | 101 | 77.5 | 66.7 | 75.0 | |
I2 | 1 | 84.0 | 57,050 | 84 | N/A | N/A | N/A | |
J | 16 | 239.8 | 110,332 | 530 | 70.8 | 40.1 | 45.0 | |
K | 33 | 95.7 | 59,720 | 411 | 51.8 | 20.0 | 23.0 | |
K1 | 15 | 94.3 | 59,877 | 166 | 85.5 | 47.9 | 57.0 | |
K2 | 4 | 96.3 | 56,597 | 128 | 85.2 | 77.7 | 75.0 | |
K3 | 3 | 98.2 | 61,322 | 111 | 92.2 | 89.5 | 88.0 | |
K4 | 5 | 94.0 | 57,865 | 106 | 93.7 | 87.2 | 89.0 | |
K5 | 6 | 98.2 | 62,154 | 144 | 82.1 | 68.2 | 68.0 | |
L | 13 | 127.9 | 75,177 | 246 | 78.2 | 50.8 | 52.0 | |
L1 | 3 | 123.7 | 74,050 | 135 | 92.6 | 88.8 | 92.0 | |
L2 | 9 | 129.3 | 75,456 | 170 | 90.1 | 72.2 | 76.0 | |
L3 | 1 | 128.0 | 76,050 | 126 | N/A | N/A | N/A | |
M | 3 | 141.0 | 81,636 | 201 | 73.5 | 63.0 | 70.0 | |
M1 | 2 | 135.0 | 80,593 | 138 | 96.6 | 96.6 | 98.0 | |
M2 | 1 | 153.0 | 83,724 | 152 | N/A | N/A | N/A | |
N | 7 | 69.1 | 42,888 | 152 | 64.1 | 45.6 | 45.0 | |
O | 5 | 124.2 | 70,651 | 151 | 90.6 | 83.3 | 82.0 | |
P | 9 | 78.8 | 47,668 | 159 | 76.1 | 42.3 | 50.0 | |
P1 | 8 | 78.4 | 47,313 | 126 | 82.9 | 52.9 | 62.0 | |
P2 | 1 | 82.0 | 50,513 | 82 | N/A | N/A | N/A | |
Q | 5 | 85.2 | 53,755 | 90 | 96.6 | 90.4 | 95.0 | |
R | 4 | 101.5 | 71,348 | 117 | 91.4 | 84.8 | 87.0 | |
S | 2 | 109.0 | 65,172 | 117 | 91.7 | 91.7 | 93.0 | |
T | 3 | 66.7 | 42,833 | 83 | 86.1 | 82.5 | 80.0 |
Cluster Averaged Shared Phamilies.
Cluster Associated Phamilies.
Cluster Cohesion Index.
List of 627 sequenced mycobacteriophages and cluster designations.
Full list of group author details.