(A) Upper panel shows Smc expression levels of strains with mutant smc alleles (BSG1002, 1007, 1008, 1045, 1046, 1047 and 1074) determined by immunoblotting using anti-Smc antibodies. Lower panel shows Coomassie staining of a SDS-PAGE gel loaded with the same whole cell extract samples to control for equal protein extraction efficiency. (B) Overnight cultures of strains (BSG1002, 1007, 1008, 1045, 1046, 1047) were spotted on SMG and NA as described before (see Figure 2—figure supplement 1). Smc locus was without mutation (‘WT’), a smc deletion (‘Δsmc’) or mutations in the Smc ATPase domain. (C) Smc ATPase mutations show slightly decreased levels of cross-linking species ‘e’ and increased levels of ‘d’. Image is identical to Figure 3B, with higher contrast.