A panel of induced pluripotent stem cells from chimpanzees: a resource for comparative functional genomics
- University of Chicago, United States
- Institució Catalana de Recerca i Estudis Avançats, Spain
- University of Michigan, United States
- Lonza Walkersville, Inc., United States
- University of California San Diego, United States
- The Scripps Research Institute, United States
- Sanford Consortium for Regenerative Medicine, United States
Abstract
Comparative genomics studies in primates are restricted due to our limited access to samples. In order to gain better insight into the genetic processes that underlie variation in complex phenotypes in primates, we must have access to faithful model systems for a wide range of cell types. To facilitate this, we generated a panel of 7 fully characterized chimpanzee induced pluripotent stem cell (iPSC) lines derived from healthy donors. To demonstrate the utility of comparative iPSC panels, we collected RNA-sequencing and DNA methylation data from the chimpanzee iPSCs and the corresponding fibroblast lines, as well as from 7 human iPSCs and their source lines, which encompass multiple populations and cell types. We observe much less within-species variation in iPSCs than in somatic cells, indicating the reprogramming process erases many inter-individual differences. The low within-species regulatory variation in iPSCs allowed us to identify many novel inter-species regulatory differences of small magnitude.
Article and author information
Author details
Reviewing Editor
- Duncan T Odom, Cancer Research UK Cambridge Institute, United Kingdom
Version history
- Received: February 19, 2015
- Accepted: June 22, 2015
- Accepted Manuscript published: June 23, 2015 (version 1)
- Version of Record published: July 15, 2015 (version 2)
Copyright
© 2015, Gallego Romero et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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A panel of induced pluripotent stem cells from chimpanzees: a resource for comparative functional genomics
eLife 4:e07103.
https://doi.org/10.7554/eLife.07103
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