(A) Proteotypic peptides for relative quantification of neurexin variants. The position of peptides within the overall domain structure of neurexin proteins is indicated (laminin-G and EGF-domains …
Note: one NRX2-alpha specific peptide (VNDGEWCHVDFQR) could not be identified by shotgun MS/MS. However, read-outs obtained with this peptide were very close to values obtained with two other …
(A, B) Relative amounts of total neurexin (NRX-pan), NRX-alpha, beta, and splice insertions across P30 mouse brain (OB = olfactory bulb, CX = cortex, HIP = hippocampus, TH = thalamus, ST = striatum, …
(A) Workflow of the preparation of Triton X-100 resistant membranes (TRM) from brain tissues for enrichment of synaptic proteins. (B) Hierarchical clustering of log-transformed protein normalized …
(A) SRM based quantification of the enrichment of neurexin proteins in TRM and PSD samples (left diagram). . In both preparations comparable enrichment (∼4 fold) could be observed. No bias in …
(A) Western blot for recombinant proteins and SRM-based quantification approach using in vitro translated heavy amino acids labeled standards fused to GFP and as heavy standards in SRM assays. For …
Peptide-specific standard curves were derived by spiking each digested synaptic protein-construct at a concentration range of five values into a digested mixture of mouse whole brain TRM protein …
(A) Ratios of normalized spectral abundance factor (NSAF) of NRX1, 2, 3-specific peptides recovered from NL1B, NL3A2, LRRTM2-affinity matrices detected in shotgun experiments. Means of the …
Lists of transitions and limits of detection and quantification for proteotypic peptides used in this study.
(A) Complete list of monitored transitions of peptides for neurexin and other proteins used in this study. (B) Complete list of Limits of detection (LOD) and quantification (LOQ) for peptides derived from in vitro expressed neurexin constructs used for absolute quantification of neurexin isoforms in this study.