(A) Time series two-photon laser scanning microscopy (TPLSM) pictures with overlaid tracks of cell motility of the alveolar area from explanted lung. Pink, blue, red, and green squares surround alveolar macrophages, patrolling monocytes, interstitial ECFP+ cells, and interstitial EGFP+ cells, respectively. (B) Time series TPLSM pictures show representative protrusive activity (upper panel) and patrolling (lower panel) behaviour by interstitial ECFP+ cells. (C) Quantification of the mean velocity and arrest coefficient of cell subsets. Bars indicate the medians. Data are pooled from six independent mice. Kruskal–Wallis tests followed by Dunn’s multiple comparison tests were performed. (D) Mean displacement ± SEM as a function of the square root of time for alveolar macrophages (AM) (pink), scanning interstitial ECFP+ cells (red), EGFP+ cells (green) (left scale), and patrolling interstitial ECFP+ cells (blue) (right scale). Coloured lines represent the linear regression of the curves. r2 and motility coefficients (MC = x²/4t) are indicated.