Unified quantitative characterization of epithelial tissue development
- Institut Curie, France
- Aix Marseille Université, France
- Meiji University, Japan
- Kyoto University, Japan
- Univsersité Paris-Diderot, France
Abstract
Understanding the mechanisms regulating development requires a quantitative characterization of cell divisions, rearrangements, cell size and shape changes, and apoptoses. We developed a multiscale formalism that relates the characterizations of each cell process to tissue growth and morphogenesis. Having validated the formalism on computer simulations, we quantifed separately all morphogenetic events in the Drosophila wing and dorsal thorax pupal epithelia to obtain comprehensive statistical maps linking cell and tissue scale dynamics. While globally cell shape changes, rearrangements and divisions all signifcantly participate in tissue morphogenesis, locally, their relative participations display major variations in space and time. By blocking division we analyzed the impact of division on rearrangements, cell shape changes and tissue morphogenesis. Finally, by combining the formalism with mechanical stress measurement, we evidenced unexpected interplays between patterns of tissue elongation, cell division and stress. Our formalism provides a novel and rigorous approach to uncover mechanisms governing tissue development.
Article and author information
Author details
Reviewing Editor
- Naama Barkai, Weizmann Institute of Science, Israel
Version history
- Received: May 6, 2015
- Accepted: November 3, 2015
- Accepted Manuscript published: December 12, 2015 (version 1)
- Accepted Manuscript updated: December 15, 2015 (version 2)
- Version of Record published: March 22, 2016 (version 3)
Copyright
© 2015, Guirao et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Unified quantitative characterization of epithelial tissue development
eLife 4:e08519.
https://doi.org/10.7554/eLife.08519
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