Unified quantitative characterization of epithelial tissue development
- Institut Curie, France
- Aix Marseille Université, France
- Meiji University, Japan
- Kyoto University, Japan
- Univsersité Paris-Diderot, France
Abstract
Understanding the mechanisms regulating development requires a quantitative characterization of cell divisions, rearrangements, cell size and shape changes, and apoptoses. We developed a multiscale formalism that relates the characterizations of each cell process to tissue growth and morphogenesis. Having validated the formalism on computer simulations, we quantifed separately all morphogenetic events in the Drosophila wing and dorsal thorax pupal epithelia to obtain comprehensive statistical maps linking cell and tissue scale dynamics. While globally cell shape changes, rearrangements and divisions all signifcantly participate in tissue morphogenesis, locally, their relative participations display major variations in space and time. By blocking division we analyzed the impact of division on rearrangements, cell shape changes and tissue morphogenesis. Finally, by combining the formalism with mechanical stress measurement, we evidenced unexpected interplays between patterns of tissue elongation, cell division and stress. Our formalism provides a novel and rigorous approach to uncover mechanisms governing tissue development.
Article and author information
Author details
Reviewing Editor
- Naama Barkai, Weizmann Institute of Science, Israel
Publication history
- Received: May 6, 2015
- Accepted: November 3, 2015
- Accepted Manuscript published: December 12, 2015 (version 1)
- Accepted Manuscript updated: December 15, 2015 (version 2)
- Version of Record published: March 22, 2016 (version 3)
Copyright
© 2015, Guirao et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 5,616
- Page views
-
- 1,623
- Downloads
-
- 110
- Citations
Article citation count generated by polling the highest count across the following sources: Crossref, Scopus, PubMed Central.
Download links
A two-part list of links to download the article, or parts of the article, in various formats.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Unified quantitative characterization of epithelial tissue development
eLife 4:e08519.
https://doi.org/10.7554/eLife.08519
Further reading
-
- Physics of Living Systems
Foraging mammals exhibit a familiar yet poorly characterized phenomenon, ‘alternation’, a pause to sniff in the air preceded by the animal rearing on its hind legs or raising its head. Rodents spontaneously alternate in the presence of airflow, suggesting that alternation serves an important role during plume-tracking. To test this hypothesis, we combine fully resolved simulations of turbulent odor transport and Bellman optimization methods for decision-making under partial observability. We show that an agent trained to minimize search time in a realistic odor plume exhibits extensive alternation together with the characteristic cast-and-surge behavior observed in insects. Alternation is linked with casting and occurs more frequently far downwind of the source, where the likelihood of detecting airborne cues is higher relative to ground cues. Casting and alternation emerge as complementary tools for effective exploration with sparse cues. A model based on marginal value theory captures the interplay between casting, surging, and alternation.
-
- Physics of Living Systems
Computational model reveals why pausing to sniff the air helps animals track a scent when they are far away from the source.
