(A) RNA expression levels of expressed lncRNAs with or without translated ORFs and mRNAs in fibroblast cells measure by RNA-seq. (B) Translation efficiency of translated ORFs in lncRNAs and canonical ORFs in mRNAs in fibroblast cells. (C,D) Relative subcellular location of translated/untranslated lncRNAs and mRNAs. RPKM values were calculated using RNA-seq data for nucleus and cytosol fractions of K562 (C) and Hepg2 (D) cells. (E–G) Relative subcellular localization of translated/untranslated pseudogenes. (H, I) mRNAs were grouped based on translation efficiency as in Figure 3D, and relative subcellular locations of mRNAs in K562 (H) and Hepg2 (I) cells were shown.