(A) RQ of the ste11 mRNA determined by quantitative RT-PCR using the ΔΔct method in the wt, set1Δ, and H3K4R strains. Each column represents the averaged value ± SEM (n = 2). a.u.: arbitrary units. Note that the wild-type control is different in the case of the set1Δ and H3K4R strains because the histone mutant is constructed in a background where only one copy of the histone H3 is retained (Figure 3—figure supplement 1C). Isogenic strains are used as control and set as 1. (B) Heterothallic wild-type, lsk1Δ, set1Δ, and set1Δ lsk1Δ strains were plated for 48 hr on mating medium before iodine staining to reveal sterility. (C) NLS-GST-CTD fusions containing wt, S2A, or S5A repeats were expressed in fission yeast from a pREP-3 plasmid. Protein extracts from the ctr and set1-TAP strains expressing these fusions were probed by Western blot using the anti-GST, anti-S5P, anti-S2P, or anti-TAP antibodies as indicated (Top panel-Total extracts). After TAP immunoprecipitation, the resulting beads were probed by Western blot using anti-GST or anti-TAP antibodies (Bottom panel-TAP IP). Right panel: a protein extract from the ctr strain expressing the NLS-GST-CTD fusion was treated or not by a phosphatase and probed by Western blot using anti-GST antibodies.