(A) Expression levels of FIL, TMO3, LFY and ANT in wild-type (WT), mp-S319 or brm-3 syd-5 inflorescence shoot apices normalized to that of EIF4A-1. Expression in WT was set to one. (B) In situ hybridization of wild-type, mp-S319 or brm-3 syd-5 inflorescence shoot apices prior to ‘pin’ formation using antisense FIL, TMO3 and LFY probes. Black arrowheads: organogenic region from where flower primordia usually arise. Figure 2—figure supplement 1 shows that MP expression is not visibly reduced in brm-3 syd-5 mutants. Inducible increase or reduction of MP function triggered increased or decreased expression of FIL, TMO3, LFY and ANT, respectively (Figure 2—figure supplement 2). (C) Anti-GFP chromatin immunoprecipitation (ChIP) to test pSYD:GFP-SYD and pBRM:BRM-GFP occupancy at pMP:MP-HA bound sites (as determined by anti-HA ChIP). For MP, BRM and SYD occupancy at the ANT locus see Figure 2—figure supplement 3. For comparison of the binding pattern of BRM, SYD and MP at the FIL, TMO3 or LFY loci see Figure 2—figure supplement 4. Control: anti-GFP or anti-HA ChIP in non-transgenic plants. NC: negative control locus (Ta3 retrotransposon).