1. Cell Biology
  2. Developmental Biology
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Coordination of planar cell polarity pathways through Spiny legs

  1. Abhijit A Ambegaonkar
  2. Kenneth D Irvine  Is a corresponding author
  1. Rutgers University, United States
Research Article
  • Cited 23
  • Views 1,542
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Cite this article as: eLife 2015;4:e09946 doi: 10.7554/eLife.09946

Abstract

Morphogenesis and physiology of tissues and organs requires planar cell polarity (PCP) systems that orient and coordinate cells and their behaviors, but the relationship between PCP systems has been controversial. We have characterized how the Frizzled and Dachsous-Fat PCP systems are connected through the Spiny-legs isoform of the Prickle-Spiny-legs locus. Two different components of the Dachsous-Fat system, Dachsous and Dachs, can each independently interact with Spiny-legs and direct its localization in vivo. Through characterization of the contributions of Prickle, Spiny-legs, Dachsous, Fat, and Dachs to PCP in the Drosophila wing, eye, and abdomen, we define where Dachs-Spiny-legs and Dachsous-Spiny-legs interactions contribute to PCP, and provide a new understanding of the orientation of polarity and the basis of PCP phenotypes. Our results support the direct linkage of PCP systems through Sple in specific locales, while emphasizing that cells can be subject to and must ultimately resolve distinct, competing PCP signals.

Article and author information

Author details

  1. Abhijit A Ambegaonkar

    Waksman Institute, Rutgers University, Piscataway, United States
    Competing interests
    The authors declare that no competing interests exist.
  2. Kenneth D Irvine

    Waksman Institute, Rutgers University, Piscataway, United States
    For correspondence
    irvine@waksman.rutgers.edu
    Competing interests
    The authors declare that no competing interests exist.

Reviewing Editor

  1. Jeremy Nathans, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, United States

Publication history

  1. Received: July 8, 2015
  2. Accepted: October 22, 2015
  3. Accepted Manuscript published: October 27, 2015 (version 1)
  4. Version of Record published: February 11, 2016 (version 2)
  5. Version of Record updated: February 8, 2017 (version 3)

Copyright

© 2015, Ambegaonkar & Irvine

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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