PHF13 is a molecular reader and transcriptional co-regulator of H3K4me2/3
- Max Planck Institute for Molecular Genetics, Germany
- Structural Genomics Consortium, Canada
- Heinrich-Pette-Institute - Leibniz Institute for Experimental Virology, Germany
- Bayer Pharma AG, Germany
- Ludwig-Maximilians-University, Germany
- Centre for Genomic Regulation, Spain
- Friedrich-Alexander-Universität Erlangen-Nürnberg, Germany
Abstract
PHF13 is a chromatin affiliated protein with a functional role in differentiation, cell division, DNA damage response and higher chromatin order. To gain insight into PHF13's ability to modulate these processes, we elucidate the mechanisms targeting PHF13 to chromatin, its genome wide localization and its molecular chromatin context. Size exclusion chromatography, mass spectrometry, X-ray crystallography and ChIP sequencing demonstrate that PHF13 binds chromatin in a multivalent fashion via direct interactions with H3K4me2/3 and DNA, and indirectly via interactions with PRC2 and RNA PolII. Furthermore, PHF13 depletion disrupted the interactions between PRC2, RNA PolII S5P, H3K4me3 and H3K27me3 and resulted in the up and down regulation of genes functionally enriched in transcriptional regulation, DNA binding, cell cycle, differentiation and chromatin organization. Together our findings argue that PHF13 is an H3K4me2/3 molecular reader and transcriptional co-regulator, affording it the ability to impact different chromatin processes.
Article and author information
Author details
Copyright
© 2016, Ho-Ryun et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 2,656
- views
-
- 685
- downloads
-
- 25
- citations
Views, downloads and citations are aggregated across all versions of this paper published by eLife.
Download links
A two-part list of links to download the article, or parts of the article, in various formats.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
PHF13 is a molecular reader and transcriptional co-regulator of H3K4me2/3
eLife 5:e10607.
https://doi.org/10.7554/eLife.10607
