(A) Location of the IE1-CTD binding site on the acidic patch of the nucleosomal surface. The histone octamer is shown as a surface representation colored according to electrostatic potential …
A stereo view of the simulated annealing (Fo-Fc) omit map showing the presence of IE1-CTD. The map is contoured at 2.5 σ level. A stick model of IE1-CTD is superimposed.
All NCP-binding peptides or protein segments, shown in a stick model superimposed onto a cartoon representation of the backbone, were superimposed onto the structure of NCP, shown in a surface …
(A–G) Raw data and fitting curves of the integrated data for the indicated peptides and NCPs are shown together with the derived KD values and fitting errors.
Bindings of full-length IE1 (A), IE1-CTD (B), and IE1 lacking CTD (C) are measured by ITC at 150 mM NaCl concentration.
(A) AUC analyses showing that IE1-CTD has little effect on the folding of the 10-nm nucleosomal array. Green and black data points represent that of 10-nm nucleosomal arrays in the absence and …
(A) Nucleosomal arrays corresponding to ~1 μg DNA were cleaved with indicated amount of micrococcal nuclease (MNase). (B) EM analysis of the reconstituted nucleosomal array (Bar: 100 nm).
Statistics of crystallographic analysis.
Data collection statistics | |
wavelength (Å) | 1.0308 |
space group | P212121 |
unit cell (Å) | a = 106.70, b = 109.47, c = 181.98 |
resolution (Å) | 30.00–2.80 (2.90–2.80) |
Rmerge | 0.133 (0.611) |
I/σI | 12.5 (3.3) |
Completeness (%) | 99.9 (100.0) |
Total/Unique reflections | 346679/52496 |
Refinement statistics | |
Rwork/Rfree | 0.195/0.244 |
rmsd bonds (Å) | 0.008 |
rmsd angles (º) | 0.935 |
No. of Atoms | |
Protein | 6116 |
DNA | 5982 |
Peptide | 104 |
Ion | 4 |
Water | 230 |
B factor (Å2) | |
Protein | 35.9 |
DNA | 87.8 |
Peptide | 56.9 |
Ion | 47.5 |
Water | 36.0 |
Ramachandran plot | |
favored | 750 (98.7%) |
allowed | 8 (1.1%) |
outlier | 2 (0.3%) |