(A) Schematic of mouse crossing to generate a CCK-iCre::R26-loxSTOPlox-eYFP mouse line (CckYFP). (B) Representative expression of CckYPF in a NTS coronal section. (C) Quantification of CckYPF-express…
(A–D) Bilateral stereotaxic injection of Cre-dependent excitatory hM3Dq-mCherry virus into the NTS of male Cck-iCre mice facilitated activation of CCKNTS neurons. (A) Schematic and Cre-mediated …
(A) Schematic of CCKNTS→ PVH targeting strategy using bilateral NTS delivery of Cre-dependent ChR2-eYFP expressing vector in Cck-iCre mice. (B) Selective eYFP expression following Cre-mediated …
(A) Schematic and Cre-mediated recombination of hM3Dq-mCherry vector. (B) Cck-iCre-hM3Dq-mCherryNTS mice were food deprived overnight and then treated with a single systemic injection of CNO (0.3 …
Representative hypothalamic coronal sections from a Cck-iCre-ChR2-eYFPNTS mouse. Scale bar represents 200 μm. 3v, third ventricle; AHA, anterior hypothalamic (hyp) area, anterior part; AHP, anterior …
(A) Schematic of mouse crossing to generate a Mc4r-t2a-Cre::tdTomato mouse line. (B) Schematic of PVH location in a coronal hypothalamic section. (C) CT values for individual Mc4r-t2a-Cre::tdTomato …
(A and D) Experimental strategies for the interrogation of the valence encoded by NTSCCK→PVH terminals. (B) Food-deprived Cck-iCre-ChR2-eYFPNTS mice exhibited a significant place preference for the …