COPI selectively drives maturation of the early Golgi

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Cite this article as: eLife 2015;4:e13232 doi: 10.7554/eLife.13232

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Abstract

COPI coated vesicles carry material between Golgi compartments, but the role of COPI in the secretory pathway has been ambiguous. Previous studies of thermosensitive yeast COPI mutants yielded the surprising conclusion that COPI was dispensable both for the secretion of certain proteins and for Golgi cisternal maturation. To revisit these issues, we optimized the anchor-away method, which allows peripheral membrane proteins such as COPI to be sequestered rapidly by adding rapamycin. Video fluorescence microscopy revealed that COPI inactivation causes an early Golgi protein to remain in place while late Golgi proteins undergo cycles of arrival and departure. These dynamics generate partially functional hybrid Golgi structures that contain both early and late Golgi proteins, explaining how secretion can persist when COPI has been inactivated. Our findings suggest that cisternal maturation involves a COPI-dependent pathway that recycles early Golgi proteins, followed by multiple COPI-independent pathways that recycle late Golgi proteins.

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Author details

Effrosyni Papanikou

Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, United States

Competing interests
The authors declare that no competing interests exist.
Kasey J Day

Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, United States

Competing interests
The authors declare that no competing interests exist.
Jotham Austin

Electron Microscopy Core Facility, The University of Chicago, Chicago, United States

Competing interests
The authors declare that no competing interests exist.
Benjamin S Glick

Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, United States

For correspondence
bsglick@uchicago.edu

Competing interests
The authors declare that no competing interests exist.

Reviewing Editor

Chris G Burd, Yale University, United States

Publication history

Received: November 20, 2015
Accepted: December 27, 2015
Accepted Manuscript published: December 28, 2015 (version 1)
Version of Record published: February 8, 2016 (version 2)

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This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.