(A) Localisation of DallyYFP in a WT third instar wing disc. Solid and dashed lines indicate the AP and DV boundaries respectively. (B) Expression of UASV5Pent with apGal4 internalised DallyYFP into …
(A–C) WT (A) and disc expressing DallyGFP in the Dpp stripe (B) immunostained for pMad. Expression of Dally in the Dpp stripe increases pMad in the centre of the disc at the expense of the …
(A) Schematic diagram of wing disc, where UAS constructs are expressed with apgal4 (green) and LexOV5Pent from the dpp stripe (dppLexA). (B–F) Third instar wing discs expressing UASGFP (B), …
(A–H) Adult wing and anterior wing margin morphology (for A–F) from flies of the indicated genotypes. Scale bars are shown in (A) and are 500 µm and 100 µm for wing and wing margin, respectively. A …
(A) Third instar DallyYFP wing disc immunostained for Pent. Pent and DallyYFP localise together in discrete puncta. (B,C) Third instar wing discs expressing UASV5Pent with apGal4. Immuno-staining …
(A) Localisation of Dlp in a WT third instar wing disc. (B) Expression of UASV5Pent with enGal4 resulted in a decrease of Dlp protein in the posterior compartment and co-localisation of Dlp with …
Inhibition of Dpp signalling in Mad mutant clones up-regulates the Dpp repressed gene brk, but does not increase Dlp protein level, suggesting that Dlp is not repressed by Dpp signalling. Scale bars …
(A–B) Third instar wing disc expressing LexOeGFPDpp under control of DppLG, immunostained for extracellular GFP to visualise spreading of Dpp. Expression of V5Pent in the dorsal compartment with …
(A–C) Third instar wing discs expressing UASDppHA (A), UASNotumV5 (B) or UASShfV5 (C) with apGal4. No reduction in DallyYFP is seen in any condition. Lower panels are enlargements of indicated …
(A) Localisation of TkvHA in a WT third instar wing disc. (B) UASV5Pent expressed with apGal4 co-localised with TkvHA in puncta, but no reduction in TkvHA protein level was seen. Arrows show …
(A) Wing disc expressing UASV5Pent with apGal4, in a background of TkvHA and DallyYFP. Lower panels are enlargements of boxed regions. Arrows indicate Dally, Tkv and Pent co-localisation. (B) Wing …
(A) Control third instar wing disc expressing LexOV5Pent with DppLHV1 LexA driver. Reduction of DallyYFP is seen in both dorsal (above dashed line) and ventral (below dashed line) regions of the …
(A) Two examples of wing discs expressing UASchcRNAi in the dorsal compartment at the Gal80ts restrictive temperature of 30°C for 24 hr. Substantial reduction in fluorescence is seen when …
(A) Adult wings of WT (left) and pent△2–5 (right). 9% of pent△2–5 wings have defects in the wing margin (n=35). Scale bar is 500 µm. (B) Adult wings of wild type (upper image) and pent△2–5 (lower …
(A–C) Control disc (A), or discs expressing DlpGFP with either V5Pent (C) or △SPV5Pent (B), which is non-functional and included to ensure equal number of UAS constructs. Expression of Pent …
Pent binds to glypicans and induces their signal-independent internalisation and lysosomal degradation. In the absence of Pent (left), glypican levels on cell surfaces increase. Elevated co-receptor …