The Arabidopsis transcription factor ABIG1 relays ABA signaled growth inhibition and drought induced senescence
- Carnegie Institution for Science, United States
Figures
Figure 1 with 1 supplement
Control of Arabidopsis Abscisic Acid Insensitive Growth 1 (ABIG1/HAT22) expression.
(A) Set of eight ORK genes (OPPOSITELY REGULATED BY REVOLUTA AND KANADI). (B) Structure of the ABIG1 gene. HD - DNA binding homeodomain, ZIP-leucine zipper responsible for homodimerization, EAR – …
Figure 1—figure supplement 1
ABIG1 mRNA levels are reduced in homozygous abig1-4 mutants.
(A) ABIG1 mRNA in wild type (Col-0) and abig1-4 mutant plants.
Figure 2
Stomatal closure and germination of abig1-1 mutant seedlings in response to exogenous ABA.
(A) Germination of wild type (wt) and mutant seeds on MS medium with 5 microMolar ABA. Germination of abi1-1, abi2-1, abi3-1 and abi4-1 mutants is ABA resistant while germination of wild type and abi…
Figure 3 with 1 supplement
Vegetative growth of abig1-1 mutant seedlings in response to exogenous ABA.
One week old wt and abig1-1 mutant plants were transferred to the ABA containing medium of varying concentration (x axis is in microMolar). Leaf number (A), petiole length of leaf three (B) and …
Figure 3—figure supplement 1
Response of abig1-4 mutants to exogenous ABA.
(A–C) One week old wt (Col-0) and abig1-1 mutant plants were transferred to ABA containing medium of varying concentration. Leaf number (A), petiole length of leaf three (B) and primary root length …
Figure 4
Induced overexpression of ABIG1 mimics ABA treatment.
(A) Wild type plants (left) grown on standard MS plates (upper row) and on plates containing 5 microMolar estradiol (lower row). Each row is a series of leaves taken from a single rosette (first …
Figure 5 with 3 supplements
abig1-1 mutants are resistant to drought.
(A) Well watered plants. Wild-type Ler (left) and abig1-1 (right) plants grown in a shared pot. (B) Plants from which water was withheld for 17 days. Wild-type (left) and abig1-1 (right) plants …
Figure 5—figure supplement 1
Response of abig1-4 mutants to drought treatment.
(A) Well watered plants. Wild-type Col-0 (left) and abig1-4 (right) plants grown in a shared pot. (B) Plants from which water was withheld for 17 days. Wild-type Col-0 (left) and abig1-4 (right) …
Figure 5—figure supplement 2
Measurement of levels of SAG113/HAI1 mRNA levels in wild type and abig1 mutants in response to exogenously added ABA.
An experiment was performed on three biological replicates, each with two technical replicates. ABA treatment was on 7 day old liquid grown seedlings treated with ABA for 120 min.
Figure 5—figure supplement 3
Response of abig1-1 mutant outcrossed to Columbia to drought.
(A) Well watered plants. Wild-type Col-0 (left) and abig1-1 (right) plants grown in a shared pot. (B) Plants from which water was withheld for 17 days. Wild-type Col-0 (left) and abig1-1 (right) …
Figure 6 with 1 supplement
Genes regulated in response to ABIG1 induction by estradiol.
(A) Genes up-regulated in response to ABIG1 induction by estradiol. (B) Genes down-regulated in response to ABIG1 induction by estradiol. Fold change relative to time 0 is given in log base 2. …
Figure 6—figure supplement 1
mRNA levels of cytokinin biosynthetic gene CYP735A2/At1g67110 in abig1 mutants and in response to exogenously added ABA.
Experiment shows the results of three biological replicates, each with three technical replicates. ABA treatment of liquid grown seven day old seedlings was for 30 min.
Figure 7
Model for ABIG1 action in the plant.
ABA concentration increases following drought. ABIG1 mRNA increases in response and, in a pathway that is distinct from the pathways controlling germination and stomatal closure, causes a reduction …
Additional files
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Supplementary file 1
RNA expression data tables.
(A) RNA sequence data for genes showing up-regulation in response to estradiol induction of XVE:ABIG1 plants. (B) RNA sequence data for genes showing down-regulation in response to estradiol induction of XVE:ABIG1 plants. (C) Expression of cytokinin network genes in response to estradiol induced activation of ABIG1. (D) Expression of ethylene network genes in response to estradiol induced activation of ABIG1. (E) Expression of abscisic acid network genes in response to estradiol induced activation of ABIG1. (F) Expression of chlorophyll degradation network genes in response to estradiol induced activation of ABIG1. (G) Expression of jasmonate network genes in response to estradiol induced activation of ABIG1. (H) Sequence of primers used for genotyping and for Q-RT-PCR.
- https://doi.org/10.7554/eLife.13768.015
