Gene sets and the numbers contained within them are indicated by the key. The revised list of amplifed genes with lower-than-expected expression were partitioned into thirds, based on genes with the greatest (top third) or smallest (bottom third) reduction in expresison compared to expectation. (A) The fraction of genes in each group for which at least three of 103 strains showed gene amplification. (B) The distribution of Buffering Scores for genes with CNV. Here, Buffering Score represents the number of 103 strains with a gene duplication divided by expression constraint (Vg/Vm, see Hose et al. for details). Higher values indicate a higher propensity for CNV despite expression constraint. The orange line indicates the median value for amplified genes with proportionate expression as a reference point. Asterisks indicate statistical significance (p<0.035) compared to the amplified genes with proportionate expression. In some cases, the trends were consistent but not significant (likely owing to small sample sets).