1. Computational and Systems Biology
  2. Evolutionary Biology
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Further support for aneuploidy tolerance in wild yeast and effects of dosage compensation on gene copy-number evolution

  1. Audrey P Gasch  Is a corresponding author
  2. James Hose
  3. Michael A Newton
  4. Maria Sardi
  5. Mun Yong
  6. Zhishi Wang
  1. University of Wisconsin-Madison, United States
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Cite this article as: eLife 2016;5:e14409 doi: 10.7554/eLife.14409

Abstract

In Hose et al., we performed a genome-sequencing survey and reported that aneuploidy was frequently observed in wild strains of S. cerevisiae. We also profiled transcriptome abundance in naturally aneuploid isolates and found that 10-30% of amplified genes, depending on the strain and affected chromosome, show lower-than-expected expression compared to gene copy number. We argued that this gene group is enriched for genes subject to one or more modes of dosage compensation, where mRNA abundance is decreased in response to higher dosage of that gene. A recent manuscript by Torres et al. refutes our prior work. Here we provide a response to Torres et al., along with additional analysis and controls to support our original conclusions. We maintain that aneuploidy is well tolerated in the wild strains of S. cerevisiae that we studied and that the group of genes enriched for those subject to dosage compensation show unique evolutionary signatures.

Article and author information

Author details

  1. Audrey P Gasch

    Laboratory of Genetics, University of Wisconsin-Madison, Madison, United States
    For correspondence
    agasch@wisc.edu
    Competing interests
    The authors declare that no competing interests exist.
  2. James Hose

    Laboratory of Genetics, University of Wisconsin-Madison, Madison, United States
    Competing interests
    The authors declare that no competing interests exist.
  3. Michael A Newton

    Genome Center of Wisconsin, University of Wisconsin-Madison, Madison, United States
    Competing interests
    The authors declare that no competing interests exist.
  4. Maria Sardi

    Laboratory of Genetics, University of Wisconsin-Madison, Madison, United States
    Competing interests
    The authors declare that no competing interests exist.
  5. Mun Yong

    Laboratory of Genetics, University of Wisconsin-Madison, Madison, United States
    Competing interests
    The authors declare that no competing interests exist.
  6. Zhishi Wang

    Department of Statistics, University of Wisconsin-Madison, Madison, United States
    Competing interests
    The authors declare that no competing interests exist.

Reviewing Editor

  1. Duncan T Odom, University of Cambridge, United Kingdom

Publication history

  1. Received: January 14, 2016
  2. Accepted: February 26, 2016
  3. Accepted Manuscript published: March 7, 2016 (version 1)
  4. Version of Record published: March 9, 2016 (version 2)

Copyright

© 2016, Gasch et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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