1. Neuroscience

Astrocytes contribute to synapse elimination via type 2 inositol 1,4,5-trisphosphate receptor-dependent release of ATP

  1. Junhua Yang
  2. Hongbin Yang
  3. Yali Liu
  4. Xia Li
  5. Liming Qin
  6. Huifang Lou
  7. Shumin Duan  Is a corresponding author
  8. Hao Wang  Is a corresponding author
  1. Zhejiang University School of Medicine, China
https://doi.org/10.7554/eLife.15043
Share this article
Cite this article
  1. Junhua Yang
  2. Hongbin Yang
  3. Yali Liu
  4. Xia Li
  5. Liming Qin
  6. Huifang Lou
  7. Shumin Duan
  8. Hao Wang
(2016)
Astrocytes contribute to synapse elimination via type 2 inositol 1,4,5-trisphosphate receptor-dependent release of ATP
eLife 5:e15043.
https://doi.org/10.7554/eLife.15043
  2. Download BibTeX
  3. Download .RIS

Abstract

Selective elimination of unwanted synapses is vital for the precise formation of neuronal circuits during development, but the underlying mechanisms remain unclear. Using inositol 1,4,5-trisphosphate receptor type 2 knockout (Itpr2−/−) mice to specifically disturb somatic Ca2+ signaling in astrocytes, we showed that developmental elimination of the ventral posteromedial nucleus relay synapse was impaired. Interestingly, intracerebroventricular injection of ATP, but not adenosine, rescued the deficit in synapse elimination in Itpr2−/− mice. Further studies showed that developmental synapse elimination was also impaired in P2ry1−/− mice and was not rescued by ATP, indicating a possible role of purinergic signaling. This hypothesis was confirmed by MRS-2365, a selective P2Y1 agonist, could also rescue the deficient of synapse elimination in Itpr2−/− mice. Our results uncovered a novel mechanism suggesting that astrocytes release ATP in an IP3R2-dependent manner to regulate synapse elimination.

https://doi.org/10.7554/eLife.15043.001

eLife digest

Neighbouring neurons connect to each other and share information through structures known as synapses. As the brain develops, many synapses turn out to be redundant. Just like trees in a garden that need to be trimmed, these redundant synapses must be pruned in order to form the right pattern of connections between different neurons. Brain cells called astrocytes play a key role in synaptic pruning, but it is unclear exactly how astrocytes coordinate this process.

One important way in which astrocytes communicate with neurons is through a process called calcium signaling, in which the movement of calcium ions into or out of the cell sets off a cascade of activity inside the astrocytes. Yang et al. have now studied developing mice that lacked a gene that is essential for calcium signaling in astrocytes. Two weeks after they were born, these mice still had redundant synapses that are normally lost after birth. However, injecting the developing brain with a substance called ATP prevented this defect and allowed synapses to be correctly pruned. This is likely to be because astrocytes also use ATP to communicate with neurons, and ATP compensated for the missing calcium signaling.

The experiments also uncovered the specific structure – called the P2Y1 receptor – on the outer surface of a neuron that ATP latches on to in order to help remove synapses. Further work is now needed to reveal how activating the P2Y1 receptor coordinates synaptic removal.

https://doi.org/10.7554/eLife.15043.002

Introduction

Synapse elimination, a process of pruning inappropriate synapses during development is essential for the formation of neuronal circuits and proper brain function (Katz and Shatz, 1996; Paolicelli et al., 2011; Sanes and Lichtman, 1999). Disruption of this process is likely involved in many neurological diseases such as schizophrenia and autism (Hoffman and McGlashan, 1997; Tang et al., 2014; Tsai et al., 2012). Although activity-dependent competition among different inputs has been suggested as the key mechanisms for synapse elimination, the underlying molecular and cellular mechanisms remain unclear. In addition to the retino-geniculate and climbing fiber-Purkinje cell pathways (Chen and Regehr, 2000; Kano and Hashimoto, 2009), the principal trigeminal nucleus-ventral posteriormedial thalamic nucleus (Pr5-VPm) connection in mice has proven to be an excellent model in which to investigate developmental synapse elimination in the central nervous system (Arsenault and Zhang, 2006). VPm neurons receive a contra-lateral Pr5 projection and relay the information to the somatosensory cortex. At an early age like postnatal day 7 (P7), each VPm neuron receives an average of 7–8 Pr5 inputs. Most of these inputs are eliminated in an activity-dependent manner during development and the majority of VPm neurons only receive a single Pr5 input by P16.

Over the past decade, emerging evidence has suggested that astrocytes not only provide structural and metabolic support to neighboring neurons, but also participate in synapse formation, synaptic transmission, and synaptic plasticity (Allen, 2014; Henneberger et al., 2010; Zhang et al., 2003). Structurally, astrocytes contact 50–90% of the synapses in a given brain region (Genoud et al., 2006; Oliet et al., 2001) and form one component of the “tripartite synapse” (Araque et al., 1999). Functionally, intracellular Ca2+ ([Ca2+]i) elevation in neighboring astrocytes can be induced by activation of the synapse, triggering the release of gliotransmitters such as ATP and D-serine that modulate synaptic efficacy and plasticity (Chen et al., 2013; Fields and Burnstock, 2006; Henneberger et al., 2010; Yang et al., 2003). It is generally accepted that astrocytes release gliotransmitters in a G-protein-coupled receptor/ inositol 1,4,5-trisphosphate receptor (GPCR/IP3R)-mediated Ca2+-dependent manner. Studies in both acute slice preparations and in vivo have shown that, in response to neuronal activity, GPCR-mediated Ca2+ signaling in astrocytes depends on the activation of IP3R2, a subunit that is solely expressed in astrocytes (Fiacco and McCarthy, 2004; Navarrete et al., 2012). Controversially, studies also indicated that IP3R2-mediated Ca2+ signaling in astrocytes may not be involved in the acute modulation of neuronal activity and synaptic transmission (Agulhon et al., 2010; Petravicz et al., 2008). Indeed, there are other Ca2+ signaling pathways in astrocytes which do not involve IP3R2. A recent study showed that although the somatic Ca2+ signaling was eliminated, Ca2+ signaling in processes was intact in the astrocyes of Itpr2−/− mice (Srinivasan et al., 2015). Therefore, IP3-induced Ca2+ increase is only the major source for somatic Ca2+ signaling in astrocytes. Recent studies have shown that astrocytes could mediate synapse elimination in a neuronal activity-dependent manner through two distinct pathways: either directly by phagocytosis through the MEGF10 and MERTK pathways, or by activating the classical complement cascade via astrocyte-derived transforming growth factor-β (TGF-β) (Bialas and Stevens, 2013; Chung et al., 2013). Here, we hypothesized that IP3R2-dependent Ca2+ signaling in astrocytes might be involved in regulating developmental synapse elimination. The results from studies in the cerebellum suggested that Ca2+-permeableα-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs) in Bergmann glia are required for the developmental elimination of climbing fiber-Purkinje cell inputs, which provides a clue that astrocytic Ca2+ signaling might be involved in synapse elimination (Iino et al., 2001). Unfortunately, it is difficult to draw an exclusive conclusion since Bergmann glia are morphologically distinct from astrocytes and detect synaptic activity at cerebellar synapses primarily though activation of Ca2+-permeable AMPARs as well as GPCRs.

By combining electrophysiological, pharmacological, and immunohistochemical methods, we discovered that selectively disturbing [Ca2+]i signaling in astrocytes using Itpr2−/− mice impaired the developmental elimination of VPm relay synapses. Intracerebroventricular injection of ATP, but not adenosine, rescued the impaired synapse elimination in these mice. We further found that developmental synapse elimination was also impaired in P2ry1−/− mice and could not be rescued by ATP. Last, the deficit of synapse elimination in Itpr2−/− mice was rescued by intracerebroventricular injection a selective P2Y1 receptors agonist MRS-2365. Overall, we provide direct evidence to show that astrocytes contribute to synapse elimination in an IP3R2-dependent manner through activation of purinergic signaling.

Results

Synapse elimination is disrupted at the VPm relay synapse in Itpr2−/− mice at P16-17

To determine the role of astrocytic Ca2+ signaling in synapse elimination, we need an effective way to selectively disrupt the function of astrocytes. In mammals, astrocytes rely on IP3R2-mediated intracellular Ca2+ signaling to perform their functions. A large literature suggested that IP3R2 is the only subtype astrocytes expressed and is not expressed in microglia and neurons in the cerebrum. Thus Itpr2−/− mice could be used to study the specific roles of Ca2+ signaling in astrocytes for synapse function (Hertle and Yeckel, 2007; Li et al., 2015; Sharp et al., 1999). In line with previous studies, we also found that IP3R2 was co-expressed with GFAP, but not bio-markers for microglia or neurons in the brain areas we tested including hippocampus (Figure 1—figure supplement 1). Using Ca2+ imaging in acute brain slices, we next found that the ATP -induced somatic [Ca2+]i elevation in the astrocytes but not in the neurons of Itpr2−/− mice (Figure 1—figure supplement 2b,c) was abolished in both of the VPm and hippocampus, confirming that Ca2+ signaling was selectively impaired in astrocytes in Itpr2−/− mice. Next, we examined developmental synapse elimination by whole-cell patch recording in acute brain slices. Interestingly, we found a marked difference in the mean number of inputs received by each VPm neuron between WT and Itpr2−/− mice at P16-18 (WT = 1.2 ± 0.02, n = 26 cells from 4 mice; Itpr2−/− = 2.1 ± 0.10, n = 40 cells from 6 mice; p<0.01, Figure 1d). In WT mice, only 27% (7 of 26) of VPm relay neurons received multiple Pr5 inputs at this age (Figure 1a,c), whereas most of these neurons (72%, 32 of 42) in Itpr2−/− mice received multiple Pr5 inputs (Figure 1b,c). VPm relay neurons receive two major excitatory inputs: from layer VI cortex and the other from the Pr5 that express vesicular glutamate transporter 1 (VGluT1) and VGluT2, respectively (Graziano et al., 2008). Each Pr5 input forms multiple synaptic contacts with VPm neurons and thus, the number of inputs indicates how many axonal projections while the VGluT2 staining represents number of synaptic terminals. The pruning of somatic innervations by Pr5 inputs in the VPm is always related to the elimination of VPm relay synapses, as showed by previous studies (Takeuchi et al., 2014; Zhang et al., 2012). To further verify that there were more synapses in KO mice, we immunostained for VGluT2. Consistent with the electrophysiological results, we observed more VGluT2 puncta around the soma as well as the total numbers of puncta in Itpr2−/− mice (Figure 1e,f), indicating that there were more synapses in KO mice. In addition, we found that neuron number did not significantly change in the VPm between WT and Itpr2−/− mice at this age (Figure 1—figure supplement 3). These results strongly suggested that astrocytic IP3R2-dependent Ca2+ signaling is required for synapse elimination.

Figure 1 with 4 supplements see all
Download asset Open asset
Developmental synapse elimination was impaired in Itpr2−/− mice at P16-17.

(a and b) Left panels, sample traces of membrane current in response to stimuli over a range of intensities in VPm neurons at P16-17 in WT (a) and Itpr2−/− mice (b). Currents recorded at +40 mV are mediated by NMDA receptors, and those at -70 mV are mediated by AMPA receptors. Right panels, peak current versus stimulus intensity for WT (a) and Itpr2−/− mice (b). (c) Distributions of the number of Pr5 axons innervating each VPm neuron during P16-17 in WT (n = 26 cells) and Itpr2−/− (n = 42 cells) mice. ***p<0.001, χ2 test. (d) Histogram of average number of inputs received by each VPm neuron at P16-17 in WT and Itpr2−/− mice. ***p<0.001, unpaired Student’s t test. Error bars indicate SEM. (e) Sample confocal images of immunostained neurons and Pr5 axonal terminals in the VPm at P16. Neurons were labeled by the NeuN antibody (green), and Pr5 axon terminals were labeled by the VGluT2 antibody (red). Inset is higher-magnification of the boxed area. Scale bar, 5 µm. (f) Quantification of VGluT2 puncta/soma (left, n = 40 cells/group) and VGluT2 puncta/neuron (right, n = 12 sections from 4 mice/group) for WT and Itpr2−/− mice. ***p<0.001, unpaired Student’s t test. Error bars indicate SEM.

https://doi.org/10.7554/eLife.15043.003

During development, the strengthening of immature synapses often concurrent with the removal of redundant inputs. In this study, we did not find any change in maximal AMPAR-EPSCs, N-methyl-D-aspartate receptor (NMDA-) EPSCs and APMAR-EPSC/NMDAR-EPSC ratios recorded at VPm relay synapses when WT mice were compared with Itpr2−/− at P16 (Figure 1—figure supplement 4). Given that the Itpr2−/− mice had more inputs and synapses, the strength of each input or individual synapse was weaker than that in WT mice (Figure 1—figure supplement 4b). These data revealed that the deficit of synapse elimination was sufficient to affect synaptic strengthening during development.

Connectivity of Pr5-VPm pathway is not affected in Itpr2−/− mice at P7

To distinguish whether the deficit in synapse elimination was due to the failure of developmental elimination or abnormal synaptogenesis at an early age, we tested the connectivity of the Pr5-VPm projection at P7. We found that the number of inputs received by VPm neurons was comparable in the WT and Itpr2−/− mice (Figure 2a–d). Neither the numbers of VGluT2 puncta per soma nor per neuron differed between WT and Itpr2−/− mice at this age, indicating similar numbers of synapses in these mice (Figure 2e,f). These results suggested that synapse formation was normal at early postnatal stage in Itpr2−/− mice. To address the possibility that the deficit in synapse elimination we found at P16 was due to a delay in development, we assessed the synapse numbers at P30 by immunostaining for VGluT2. We found that even at P30, there were more Pr5-VPm synapses in Itpr2−/− mice than in WT mice (Figure 2—figure supplement 1a,b). Therefore, knockout of IP3R2 disrupted developmental synapse elimination at the VPm relay synapses.

Figure 2 with 1 supplement see all
Download asset Open asset
Connectivity of Pr5-VPm pathway was comparable in WT and Itpr2−/− mice at P7.

(a and b) Left panels, sample traces showing membrane current in response to stimuli at a range of intensities in VPm neurons at P7 in WT (a) and Itpr2−/− (b) mice. Right panels, peak current versus stimulus intensity for WT (a) and Itpr2−/− mice (b). (c) Distributions of the number of Pr5 axons innervating each VPm neuron at P7 did not differ between WT (n = 33 cells) and Itpr2−/− (n = 35 cells) mice. p=0.78, χ2 test. (d) Histogram of average number of inputs received by each VPm neuron at P7 in WT and Itpr2−/− mice (WT = 4.8 ± 0.3, n = 33; Itpr2−/− = 4.9 ± 0.3, n = 33). p=0.74, unpaired Student’s t test. (e) Sample confocal images of immunostained neurons and Pr5 axonal terminals in the VPm at P7 in WT and Itpr2−/− mice. Neurons were visualized with the NeuN antibody (green), and Pr5 axonal terminals were labeled by the VGluT2 antibody (red). Inset is higher-magnification of the boxed area. Scale bar, 5 µm. (f) Quantification of VGluT2 puncta/soma (left, n = 24 cells/group,) and VGluT2 puncta/neuron (right, n = 9 sections from 3 mice/group) for WT and Itpr2−/− mice. p>0.05, unpaired Student’s t test. Error bars indicate SEM.

https://doi.org/10.7554/eLife.15043.008

Intracerebroventricular injection of ATP from P11 to P15 rescued the deficit in synapse elimination in Itpr2−/− mice

Increasing evidence suggests that astrocytes release a number of gliotransmitters, such as glutamate, ATP, and D-serine to regulate synaptic transmission and synaptic plasticity (Chen et al., 2013; Henneberger et al., 2010; Jourdain et al., 2007). Among these gliotransmitters, we were particularly interested in ATP and assumed that it might play a role in synapse elimination for the following reasons: 1) the basal ATP levels are reduced in Itpr2−/− mice (Cao et al., 2013); 2) astrocytes release ATP in a Ca2+-dependent manner (Lalo et al., 2014; Zhang et al., 2007); and 3) ATP regulates synaptic plasticity (Chen et al., 2013). We then tested whether the basal ATP level in the VPm changes during development at the time when exploratory activity increases in WT mice. A significant increase in the basal ATP level occurred at P18 compared with that at P7 in WT mice (Figure 3a). This developmental up-regulation of the basal ATP level was absent in Itpr2−/− mice (Figure 3a). In addition, we found that the basal ATP level was comparable between WT and Itpr2−/− mice at P7 but was significantly reduced in KO mice at P18 (Figure 3a). These results suggested that, during development, the basal ATP level increases in WT but not in Itpr2−/− mice. Next, we tested whether the deficit of synapse elimination in Itpr2−/− mice could be rescued by compensatory ATP. To achieve this goal, we implanted a cannula in the left lateral ventricular of the brain (Figure 3b) and found that intracerebroventricular injection of ATP from P11 to P15 rescued the synapse elimination deficit (Figure 3e,i,l,m). At P16-17, unlike aCSF-treated mice, the majority of VPm neurons in Itpr2−/− mice that had received ATP (50 µM) treatment was innervated by a single Pr5 input (Figure 3l). Immunostaining for VGluT2 also revealed that the number of synapses significantly decreased in Itpr2−/− mice with ATP treatment (Figure 3p,q). These results indicate that ATP treatment is sufficient to rescue synapse elimination deficit in Itpr2−/− mice and the removal of redundant synapses is independent of IP3R2-dependent Ca2+ signaling. Cannula implantation caused injury may induce inflammatory responses of astrocytes and microglia differentially between aCSF and ATP treated-Itpr2−/− mice, thereby affected the rescue effects. We found that reactive astrocytes and activated microglia around the site of cannula placement in aCSF and ATP treated-Itpr2−/− mice were identical (Figure 3—figure supplement 1), and ruled out this possibility. We next applied a low dose ATP (5 µM) and found that the impaired synapse elimination cannot be rescued in Itpr2−/− mice, suggesting the rescue effect of ATP was in a dose-dependent manner (Figure 3n,o).

Figure 3 with 1 supplement see all
Download asset Open asset
Intracerebroventricular injection of ATP from P11 to P15 rescued the synapse elimination deficit in Itpr2−/− mice.

(a) Basal ATP levels at P8 and P17-19 in WT and Itpr2−/− mice. *p<0.05, two-way ANOVA followed by Bonferroni post hoc test, n = 5 mice per age. n.s, not significant. Error bars indicate SEM. (b) Schematic illustrating the cannula was implanted to the left lateral ventricle. (c) Experimental design of aCSF, ATP (50 µM), ATPγS (50 µM), and adenosine (50 µM) administration and analysis. Injections were given twice per day from P11 to P15. (d-g) Sample traces showing membrane current in response to stimuli with a range of intensities in VPm neurons from Itpr2−/− mice at P16-17 with a short period of aCSF (d), ATP (e), ATPγS (f) and adenosine (g) treatment. (h-k) Peak current versus stimulus intensity for aCSF (h), ATP (i), ATPγS (j) and adenosine (k) treatment in Itpr2−/− mice. (l) Distributions of the number of Pr5 axons innervating each VPm neuron at P16-17 with aCSF (n = 29 cells), ATP (n = 32 cells), adenosine (n = 27 cells), and ATPγS (n = 32 cells) treatment in Itpr2−/− mice. **p<0.01, ***p<0.001, χ2 test. (m) Histogram of average number of inputs received by each VPm neuron at P16-17 in WT and Itpr2−/− mice followed by different injections. ***p<0.001, one-way ANOVA followed by Bonferroni post hoc test. Error bars indicate SEM. (n) Distributions of the number of Pr5 axons innervating each VPm neuron at P16-17 with different concentrations of ATP treatment in Itpr2−/− mice. ***p<0.001, χ2 test. (o) Histogram of average number of inputs received by each VPm neuron at P16-17 in Itpr2−/− mice followed by different concentrations of ATP injection. ***p<0.001, unpaired Student’s t test. (p) Sample confocal images of immunostained neurons and Pr5 axon terminals in the VPm at P16. Inset is higher-magnification of the white boxed area. Scale bar, 5 µm. (q) Quantification of VGluT2 puncta/soma (left, n = 40 cells/group) and VGluT2 puncta/neuron (right, n = 9 sections from 3 mice/group) for WT, Itpr2−/−, ATP and adenosine-treated Itpr2−/− mice. ***p<0.001, one-way ANOVA. Error bars indicate SEM.

https://doi.org/10.7554/eLife.15043.010

Given that ATP is readily hydrolyzed, and to exclude the possibility that the degradation products of ATP play a role in synapse elimination, we injected the non-hydrolyzable ATP analog ATPγS and found that, ATPγS (50 µM) also rescued the synapse elimination impairment in Itpr2−/− mice (Figure 3f,j,l,m). These results suggested that astrocytes mediate synapse elimination via the Ca2+-dependent release of ATP. Since adenosine is a degradation product of ATP and could modulate synaptic plasticity by activating adenosine receptors (Pascual et al., 2005), we set out to exclude the possibility that adenosine rescued the synapse elimination deficit in Itpr2−/− mice. We found intracerebroventricular injection of adenosine (50 µM) throughout P11-P15 had no effect on Pr5-VPm connectivity at P16 in these mice (Figure 3g, k, l, m). Thus, the synapse elimination deficit in Itpr2−/− mice was rescued by ATP, but not adenosine.

ATP modulates synapse elimination through activation of P2Y1 receptors

Having shown that ATP rescued the synapse elimination deficit at the VPm relay synapses in Itpr2−/− mice, the next question is how this process occurs. In the CNS, ATP could activate purinegic signaling through P2X or P2Y receptors to modulate synapse function. Particularly, P2Y1 receptors have been shown participated in ATP-induced heterosynaptic long-term depression (LTD) in hippocampus (Chen et al., 2013), and LTD has been suggested correlated to synapse elimination (Bastrikova et al., 2008; Wiegert and Oertner, 2013). To test the possibility of involvement of P2Y1 receptors in synapse elimination at the VPm, we first examined the expression pattern of P2Y1 receptors and found that unlike in the hippocampus (Zhu and Kimelberg, 2004), they were only expressed in neurons in the VPm (Figure 4—figure supplement 1). We next found that ATP-induced somatic Ca2+ elevation in astrocytes was impaired in hippocampus, but not in the VPm in P2ry1−/− mice (Figure 4—figure supplement 2), further indicated that the expression pattern of P2Y1 receptors might be different in hippocampus when compared with that in the VPm. In line with immunostaining results, we also found that ATP-induced Ca2+ elevation in VPm neurons of P2ry1−/− mice was impaired (Figure 4—figure supplement 2). These results suggested that even in the VPm, the expression pattern of P2Y1 may have specificity among different cell types. Next, we determined whether synapse elimination was disrupted in P2ry1−/− mice. Compared to WT mice, the majority of VPm neurons in P2ry1−/− mice received multiple Pr5 inputs at P16 (WT = 30%, 13 of 33 cells; P2ry1−/− = 72%, 34 of 47 cells, Figure 4g). Consistent with the electrophysiological results, we confirmed the synapse elimination deficit in P2ry1−/− mice with immunostaining for VGluT2 (Figure 4i). There was more VGluT2 staining around the somata of VPm neurons in P2ry1−/− mice than in WT mice (Figures 4i,j). Therefore, synapse elimination was also impaired in P2ry1−/− mice. To address the abnormal of synaptic connectivity in P2ry1−/− mice at P16 was actually due to developmental failure, we tested Pr5-VPm synapses at P7. We found that the connectivity of Pr5-VPm pathway was comparable between WT and P2ry1−/− mice at P7 (Figure 4—figure supplement 3). In addition, the synapse elimination deficit in these mice was not rescued by intracerebroventricular injection of ATP from P11 to P15 (Figure 4c, f, g, h). These results suggested that synapse elimination promoted by ATP may depend on P2Y1 receptors. If this is the case, one direct evidence would be the rescue of the synapse elimination defect with application of the P2Y1 agonist in Itpr2−/− mice. We next found that the deficit of synapse elimination was rescued by intracerebroventricular injection of a selective P2Y1 receptors agonist, MRS-2365 (20 µM) from P11 to P15 (Figure 5). Unlike aCSF treatment, the majority of VPm neurons in Itpr2−/− mice with treatment of MRS-2365 innervated by a single Pr5 input (Figure 5c). The mean number of inputs received by each VPm neuron was significantly decreased in Itpr2−/− mice with intracerebroventricular injection of MRS-2365 (2.0 ± 0.76 MRS-2365 versus 1.33 ± 0.48 aCSF, Figure 5d). Taken together, these data confirmed that ATP promotes synapse elimination via P2Y1 receptors.

Figure 4 with 3 supplements see all
Download asset Open asset
Synapse elimination was also impaired in P2ry1−/− mice at P16-17 and cannot be rescued by ATP.

(a-c) Sample traces of membrane current in response to stimuli at a range of intensities in VPm neurons at P16-17 in WT (a), P2ry1−/− (b), and P2ry1−/− with ATP injection (c) mice. (d-f) Peak current versus stimulus intensity for WT (d), P2ry1−/− (e), and P2ry1−/− with ATP injection (f) mice. (g) Distributions of the number of Pr5 axons innervating each VPm neuron at P16-17 in WT (n = 33 cells), P2ry1−/− (n = 47 cells) and P2ry1−/− with ATP injection (n = 39 cells) mice. **p<0.01, χ2 test. (h) Average number of Pr5 axonal inputs received by individual VPm relay neurons significantly increased in P2ry1−/− mice compared to WT controls. ***p<0.001 by one-way ANOVA. (i) Sample confocal images of immunostained neurons and Pr5 axon terminals in the VPm at P16. Neurons were visualized with the NeuN antibody (green), and Pr5 axon terminals were labeled by the VGluT2 antibody (red). Inset is higher-magnification of the boxed area. Scale bar, 5 µm. (j) Quantification of VGluT2 puncta/soma (left, n = 42 cells/group) and VGluT2 puncta/neuron (right, n = 9 sections from 3 mice/group) in WT and P2ry1−/− mice. ***p<0.001, unpaired Student’s t test. Error bars indicate SEM.

https://doi.org/10.7554/eLife.15043.012
Figure 5
Download asset Open asset
Synapse elimination was rescued by P2Y1 receptor agonist MRS-2365 in Itpr2−/− mice.

(a and b) Left panels, sample traces of membrane current in response to stimuli over a range of intensities in VPm neurons at P16-17 in aCSF (a) and MRS-2365 (b) treated-Itpr2−/− mice. Right panels, peak current versus stimulus intensity for aCSF (a) and MRS-2365 (b) treated-Itpr2−/− mice. (c) Distributions of the number of Pr5 axons innervating each VPm neuron during P16-17 in aCSF (n = 32 cells) and MRS-2365 treated-Itpr2−/− mice(n = 30 cells). ***p<0.001, χ2 test. (d) Histogram of average number of inputs received by each VPm neuron at P16-17 in aCSF (n = 32 cells) and MRS-2365 (n = 30 cells) treated-Itpr2−/− mice. ***p<0.001, unpaired Student’s t test. Error bars indicate SEM.

https://doi.org/10.7554/eLife.15043.016

Discussion

Here, by using electrophysiological recording in transgenic mice, we found that astrocytes contribute to synapse elimination through Ca2+-dependent release of ATP and activation of P2Y1 receptors. Specifically, we demonstrated that (1) developmental synapse elimination in the VPm was impaired in the Itpr2−/− mice; (2) intracerebroventricular injection of ATP and ATPγS, but not adenosine, from P11 to P15, rescued the synapse elimination deficit in Itpr2−/− mice; (3) P2ry1−/− mice also showed impaired synapse elimination and this could not be rescued by ATP; and (4) deficient of synapse elimination in Itpr2−/− mice was rescued by intracerebroventricular injection of MRS-2365, a selective P2Y1 receptors agonist, from P11 to P15.

In the CNS, neurons make redundant synaptic connections early in development, and most of inappropriate synapses are eliminated later to form precise neural circuitry (Lichtman and Colman, 2000; Luo and O'Leary, 2005). This process is believed to largely rely on neuronal activity, while the underlying mechanisms are still unclear. Recent studies have shown that astrocytes show a transient [Ca2+]i elevation, which depends on the GPCR/IP3R pathway, in response to neuronal activity and subsequently release gliotransmitters to modulate synaptic transmission and plasticity (Araque et al., 2014). Therefore, astrocytic [Ca2+]i elevation is a consequence of neuronal activity. Based on these reasons, we proposed that activity-dependent competition for synapse elimination involves both postsynaptic neuronal activation and astrocytic [Ca2+]I elevation. A large literature has shown that GPCR/IP3R-dependent Ca2+ signaling is critical for astrocytes to release gliotransmitters and modulate synaptic transmission. Conversely, recent studies from McCarthy’s laboratory have suggested that IP3R2-mediated Ca2+ signaling in astrocytes may not be involved in the acute modulation of neuronal activity and synaptic transmission (Fiacco et al., 2007; Petravicz et al., 2008). Two studies have shown that there were still some Ca2+ fluctuations occurring in astrocytes process, although the somatic Ca2+ signaling was eliminated in Itpr2−/− mice (Kanemaru et al., 2014; Srinivasan et al., 2015). These results suggested that Ca2+ signaling was not totally removed in these mutants. In line with previous studies, we found that the lack of ATP-induced Ca2+ increase in astrocytes soma in these mutants. Our studies strongly suggested that astrocytic IP3R2-dependent somatic Ca2+ signaling is required for developmental synapse elimination. Considering the dynamic and complexity, the astrocytic Ca2+ signaling in the process and the soma may play different roles in gliotransmitter release and regulating neuronal functions (Volterra et al., 2014).

It is important to note that synapse elimination was only partly impaired in the Itpr2−/− and P2ry1−/− mice. Although we found both anatomical and electrophysiological evidence that these mice had significant deficits in developmental synapse elimination, some degree of synaptic pruning still occurred during P7 to P16, suggesting that there are multiple mechanisms for controlling synapse elimination. Indeed, synaptic pruning in the VPm relay synapses occurs at several developmental stages (Wang and Zhang, 2008). Before P10, pruning is insensitive to whisker deprivation, but then later, the elimination of redundant inputs is largely dependent on somatosensory experience. In the mutants, such as Itpr2−/− mice, the nature of sensory information transmitted from whisker to the VPm might be altered, thus causes the defects of synapse elimination. We found surprisingly, the connectivity between Pr5 and VPm in Itpr2−/− mice undergone ATP and ATPγS treatments restored to WT mice level. These results suggested that no matter sensory information had been changed or not, ATP was sufficient to rescue the impairment of synapse elimination in Itpr2−/− mice. However, the rescue effect of ATP may occur at a site between the whisker and VPm, and this possibility could not be ruled out by our study.

It is about P13 that mice open their eyes and start to use their vibrissae to explore the environment. Accompanying this behavioral change, we found a significant enhancement of the basal ATP level in the VPm of WT mice between P7 and P18 (Figure 3a). However, the basal ATP level failed to be up-regulated in Itpr2−/− mice during development. In line with previous studies (Cao et al., 2013), we also found a decreased basal ATP level in the brain of Itpr2−/− mice. ATP can be released by both neurons and glia. The decreased ATP level in Itpr2−/− mice was most likely due to astrocytes since IP3R2 was not expressed in neurons and [Ca2+]i elevation did not change in neurons. Although ATP itself is a signaling molecule by activating P2X or P2Y receptors, the degraded form adenosine is also a ligand for adenosine receptors and causes synaptic depression (Wu and Saggau, 1994). The fact that adenosine injection through P11-15 did not rescue the synapse elimination deficit in Itpr2−/− mice excludes the possibility of a role for adenosine in synapse elimination. In addition to being a signal molecule, ATP is also energy currency in the brain and the phenotype of Itpr2−/− mice could be a result of global metabolic stress. This possibility is very unlikely because either ATPγS, a non-hydrolyzable ATP analog or one selective P2Y1 receptors agonist MRS-2365 could rescue the deficit of synapse elimination in Itpr2−/− mice. In our study, we concluded that ATP was not acting as an energy supply to play a role in synapse elimination.

Although both of Itpr2−/− and P2ry1−/− mice showed deficits of developmental elimination of relay synapses at the VPm, it is not necessary that they are mechanistically related. That is, the phenotypes in these two mutants are two independent events. The results of P2Y1 receptors agonist injection rescued the deficient of synapse elimination in Itpr2−/− mice provided a direct linkage between IP3R2-dependent releasing of ATP and its downstream purinergic pathway. Unlike IP3R2, studies have shown that P2Y1 receptors are widely expressed in astrocytes, microglia and neurons. Thus, loss function of P2Y1 receptors in all three types of cell may contribute to the phenomenon of impaired synapse elimination in P2ry1−/− mice. First, P2Y1 receptors have been shown to be expressed by astrocytes (Pascual et al., 2012), therefore the finding that P2ry1−/− mice has impaired synapse elimination could be due to a failure to increase calcium levels in astrocytes in response to ATP. This possibility could be excluded by the results of astrocytes in the VPm of P2ry1-/- mice responded to ATP with a similar increase in intracellular Ca2+ when compared to cells in the WT mice (Figure 4—figure supplement 2). Additionally, we found that P2Y1 receptors were not expressed by astrocytes in the VPm (Figure 4—figure supplement 1a), but abundantly expressed in the hippocampus astrocytes (data not shown). Second, P2Y1 receptors have been reported to be expressed in microglia and ATP activates microglia. The phenotype of synapse elimination defects in P2ry1-/- mice could be explained by injection of ATP activates microglia-mediated synapse elimination. At present, there were some mRNA and functional clues showing that microglia expressed P2Y1 receptors, whereas the protein evidence was very few (Fields and Burnstock, 2006). In our study, we did not find any P2Y1 protein expression on microglia in both of hippocampus (data not shown) and the VPm (Figure 4—figure supplement 1b). Therefore, the protein level of P2Y1 receptors expressed on microglia in the VPm, if any, was very low. However, we still could not totally exclude the possibility that microglia may contribute to synapse elimination through P2Y1 receptors. Further studies by using cell-type specific knockout mice are needed to answer this question. Last, ATP-mediates synapse elimination may occur through activation of neuronal P2Y1 receptors. This speculation is most likely due to P2Y1 receptors are abundantly expressed in VPm neurons (Figure 4—figure supplement 1c). In addition, our Ca2+ imaging data showing that ATP-evoked Ca2+ response in VPm neurons was significantly decreased in P2ry1-/- mice. Our findings strongly suggest that ATP may activate neuronal P2Y1 receptors to promote synapse elimination. This hypothesis is supported by a previous study showing that activation of neuronal P2Y1 receptors is required for astrocytes derived ATP mediated LTD in hippocampus (Chen et al., 2013). Due to a close linkage proposed between LTD and synapse elimination (Wiegert and Oertner, 2013), one possible model is that ATP and downstream purinergic signaling could recognize unwanted synapses. Certainly, further studies are needed to elucidate the whole picture of pathways that how activation of purinergic signal mediating synapse elimination.

Materials and methods

Animals

IP3R2-knockout (Itpr2−/−) mice were a kind gift from Dr. Ju Chen (University of California, San Diego) and maintained as a heterozygous line. Heterozygous (Itpr2+/−) mice were interbred to generate homozygous full-mutant mice (Itpr2−/−) and WT littermate controls (Itpr2+/+), which were used in experiments. Cx3cr1GFP/+ mice，hGFAP-GFP mice, and heterozygous P2ry1−/− mice were obtained from Jackson Laboratories.

Slice preparation

Request a detailed protocol

Sagittal slices were obtained as previously described (Wang and Zhang, 2008). Mice and littermate controls were anesthetized with sodium pentobarbital, and then perfused with ice-cold oxygenated slicing solution. The brain was removed rapidly and immersed in ice-cold slicing solution containing (in mM): 110 choline chloride, 7 MgCl2·6H2O, 2.5 KCl, 0.5 CaCl2·H2O, 1.3 NaH2PO4, 25 NaHCO3, 20 glucose, saturated with 95% O2 and 5% CO2. The brain was cut into 300 µm slices on a vibratome (MicromHM650V). Slices were allowed to recover for 40 min at 32°C and then at room temperature for recording in artificial cerebrospinal fluid (aCSF) containing (in mM): 125 NaCl, 2.5 KCl, 2 CaCl2·H2O, 1.3 MgCl2·6H2O, 1.3 NaH2PO4, 25 NaHCO3, 10 glucose. Oxygen was continuously supplied during recovery and recording. To block inhibitory synaptic transmission, picrotoxin (100 µM) was added to the bath.

Electrophysiology

Request a detailed protocol

All recordings were made at room temperature in a submerged recording chamber with constant aCSF perfusion. Patch electrodes had a resistance of 2–4 MΩ when filled with an internal solution containing (in mM): 110 Cs methylsulfate, 20 TEA-Cl, 15 CsCl, 4 ATP-Mg, 0.3 GTP, 0.5 EGTA, 10 HEPES, 4.0 QX-314, and 1.0 spermine (pH adjusted to 7.2 with CsOH, 290–300 mOsm with sucrose). Whole-cell voltage-clamp recordings were made from the soma of VPm neurons with an Axopatch 200B amplifier and Digidata 1322A with pCLAMP 8.1 software (Molecular Devices). Cells were visualized under an upright microscope (BX51WI, Olympus) with infrared optics. Signals were filtered at 2 kHz and digitized at 10 kHz. The series resistance (Rs) was <20 MΩ with no compensation and data were discarded when this varied by ≥ 20%.

To determine the numbers of inputs to a VPm neuron, we recorded evoked EPSCs from the same cells at holding potentials of -70 and +40 mV cell over a wide range of stimulus intensity. A concentric electrode (World Precision Instruments) was placed on the medial lemniscal fiber bundle and stimuli (typically from 20 to 600 µA, 100 µs) were delivered at 0.1 Hz via a Grass S88 stimulator. To distinguish lemniscal synaptic responses from corticothalamic responses, paired-pulse stimulation with an interval of 100 ms was used. First, we used increments of 50–100 µA to search for step numbers. Then we used small increments of 1–10 µA near each transition point to ensure that it was actually a single step. After that, we applied a stronger stimulus, the intensity of which was at least twice than the last step.

Extracellular ATP measurement

Request a detailed protocol

The concentration of extracellular ATP was determined with a bioluminescent ATP assay kit (Sigma), as previously described (Zhang et al., 2007). The ectonucleotidase inhibitor dipyridamole (10 µM) was added to the extracellular solution throughout the experiment to decrease ATP hydrolysis. Luminescence was measured with a luminometer (Varioskan Flash, Thermo) according to the manufacturer’s instructions. A calibration curve was constructed from standard ATP samples and the luminescence of the incubated medium was measured as the background ATP level. To measure the ATP content in acute VPm slices, they were incubated in oxygenated ACSF for 10 min at room temperature. The ACSF was then collected for ATP assay. For quantification, the amount of protein was used for normalization and was determined using the Enhanced BCA protein assay kit (Beyotime, China).

Immunostaining

View detailed protocol

Animals were anesthetized with sodium pentobarbital, and perfused with 0.9% saline followed by ice-cold 4% paraformaldehyde. Brains were removed and post-fixed overnight in 4% paraformaldehyde at 4°C and then transferred to 30% sucrose in PBS at 4°C for 2 days. Sagittal sections 30 or 40 µm thick were cut on a microtome (Leica CM 1850). Sections were dried, washed three times in 0.01M PBS and with 0.3% Triton-100X in 0.1M PBS (40 min) or frozen methanol (10 min at -20°C), then blocked with 10% BSA for 1 hr at room temperature. Sections were incubated with primary antibodies as follows: VGluT2 (guinea-pig polyclonal, 1:2000, Millipore), NeuN (monoclonal mouse, 1:500; Millipore), GFAP (rabbit, 1:500, Chemicon), GFAP (monoclonal mouse, 1:500; Synaptic System), Iba1 (polyclonal rabbit, 1:200; Chemicon), IP3R2 (polyclonal rabbit, 1:200; Santa Cruz), P2Y1 (polyclonal rabbit, 1:200; Abcom) at 4°C for 12–24 hr. Secondary Alexa-conjugated antibodies were added at 1:1000 in 0.1 M PBS for 2 hr at room temperature. Images were captured using an Olympus FV-1200 inverted confocal microscope.

Brain slice Ca2+ imaging

Request a detailed protocol

In brief, for Ca2+ imaging in hippocampus, WT, Itpr2−/− mice, and P2ry1-/- (P13-P16) were sacrificed and coronal slices of hippocampus were cut and incubated in ACSF at room temperature, saturated with 95% O2 and 5% CO2. For Ca2+ imaging in VPm, sagittal slices containing VPm were cut. Slices were incubated with 10 µM Fluo-4/AM (Invitrogen) or Cal-250 (AATBioquest) for 45 min at room temperature in oxygenated ACSF. In these conditions, most of the loaded cells in the stratum radiatum and VPm were astrocytes as confirmed by SR101 staining. CA1, dentate gyrus, and VPm neurons were also loaded in these conditions. Before imaging, slices were transferred to stain-free ACSF for at least 10 min to wash out the stain. Images were acquired every 3 s using an Olympus FV-1200 confocal microscope. Average fluorescence intensity was measured from analysis boxes over the cell-bodies of astrocytes and neurons. When the baseline was stable, ATP (100 µM) was added to evoke intracellular Ca2+ release from astrocytes and neurons. Increases in the fluorescence intensity over baseline were calculated for each trace and are reported as ΔF/F0.

Quantification of VGluT2

Request a detailed protocol

Confocal images were collected with a 60× objective (NA 1.3, water) on an Olympus FV-1200 inverted confocal microscope. Z-stacks were obtained by 10 consecutive steps with 0.5 µm interval thickness. To determine the VGluT2 puncta on the soma of a thalamic VPm neuron, data were opened with Fluoview software (Olympus) and then exported as 8-bit tiff files. Neurons were randomly selected and VGluT2 puncta were counted manually using the Cell Counter plugin of ImageJ. In most cases, the VGlut2 puncta are separated from each other. Sometimes there are multiple puncta that stay very close, but we can still discriminate them by the little gaps among them. Occasionally, there is a big puncta that has no gap, which is then referred as one puncta. We count the puncta that contact the NeuN as puncta/soma. To quantify the average VGluT2 puncta per neuron, the density of VGluT2 puncta was analyzed using the Analyze Particles command in ImageJ (version 1.43). Thalamic VPm neuron number was counted manually using the Cell Counter plugin of ImageJ. The puncta/neuron is then determined by all the puncta number divided by all neuron number in a given image.

Neuron density quantification in the VPm

Request a detailed protocol

For quantification of neuron density, 9 sections from 3 different animals (3 sections per animal) were used. The VPm was acquired by using a 10×objective (NA 0.4). Thalamic VPm neuron numbers were counted manually by using the Cell Counter plugin of Image J. To calculate the density of neurons, only the area of the VPm was measured.

Cannula implantation and pharmacological experiments

Request a detailed protocol

Mice at P10 were deeply anaesthetized with isoflurane and placed into a stereotactic apparatus. A 30-gauge stainless steel infusion cannula (RWD life science) was implanted unilaterally in the left lateral ventricle (-0.4 mm from bregma, 0.9 mm lateral from midline, and 2.5 mm vertical from the cortical surface) and was fixed to the skull with dental cement. After one day recovery, drugs including aCSF, ATP (5 or 50 µM, 2 µl), adenosine (50 µM, 2 µl), ATPγS (50 µM, 2 µl), MRS-2365 (20 µM, 2 µl) were delivered to lateral ventricle slowly by using a microsyringe pump which was connected to the infusion cannula by a PE20 tube. Injections were given twice per day at the interval of 12 hr from P11 to P15. After experiments, the location of the cannula was verified histologically and discarded the animal which did not target to lateral ventricle.

Inflammatory response quantification

Request a detailed protocol

To analysis the inflammatory responses of astrocytes and microglia, fixed sections were immunolabeled for GFAP and Iba1. For quantification the maximum distance of reactive cells to the injury site, three images were collected from each animal on a confocal microscopy by using 1 µm z-step. Astrocytes in the cortex express a low level of GFAP in normal situation. They were very sensitive to injury as indicated by an increased GFAP expression. Therefore, the distance of GFAP positive cells to injury site was measured as the distance of inflammatory responses. For quantification of reactive astrocyte, the number of GFAP positive cells within 150 µm from the injury site was counted. For quantification of microglia activation, the number of Iba1+ microglia within 150 µm from the injury site was counted. Then the percentage of amedoid-like activated microglia was calculated.

Statistical analysis

Request a detailed protocol

All results are presented as mean ± SEM. GraphPad Prism 5 (La Jolla, CA) software was used for all statistical analyses. Differences between two groups were evaluated by un-paired or paired Student’s t-test. For multi-group comparisons, one-way ANOVA with Tukey’s multiple-comparisons test was used. Two-way ANOVA followed by the Bonferroni post hoc test was used for Figure 3A. Frequency distributions were analyzed with χ2 test. The significance level was set at p<0.05.

References

Article and author information

Author details

  1. Junhua Yang

    Department of Neurobiology, Key Laboratory of Medical Neurobiology of Ministry of Health of China, Key Laboratory of Neurobiology, Zhejiang University School of Medicine, Hangzhou, China
    Contribution
    JY, Drafting the article, Final approval of the version to be published, Conception and design, Acquisition of data, Analysis and interpretation of data
    Contributed equally with
    Hongbin Yang
    Competing interests
    The authors declare that no competing interests exist.

  2. Hongbin Yang

    Department of Neurobiology, Key Laboratory of Medical Neurobiology of Ministry of Health of China, Key Laboratory of Neurobiology, Zhejiang University School of Medicine, Hangzhou, China
    Contribution
    HY, Drafting the article, Final approval of the version to be published, Conception and design, Acquisition of data, Analysis and interpretation of data
    Contributed equally with
    Junhua Yang
    Competing interests
    The authors declare that no competing interests exist.

  3. Yali Liu

    Department of Neurobiology, Key Laboratory of Medical Neurobiology of Ministry of Health of China, Key Laboratory of Neurobiology, Zhejiang University School of Medicine, Hangzhou, China
    Contribution
    YL, Discussing the revision of the article, Final approval of the version to be published, Acquisition of data, Analysis and interpretation of data
    Competing interests
    The authors declare that no competing interests exist.

  4. Xia Li

    Department of Neurobiology, Key Laboratory of Medical Neurobiology of Ministry of Health of China, Key Laboratory of Neurobiology, Zhejiang University School of Medicine, Hangzhou, China
    Contribution
    XL, Discussing the revision of the article, Final approval of the version to be published, Contributed unpublished essential data or reagents
    Competing interests
    The authors declare that no competing interests exist.

  5. Liming Qin

    Department of Neurobiology, Key Laboratory of Medical Neurobiology of Ministry of Health of China, Key Laboratory of Neurobiology, Zhejiang University School of Medicine, Hangzhou, China
    Contribution
    LQ, Discussing the revision of the article, Final approval of the version to be published, Contributed unpublished essential data or reagents
    Competing interests
    The authors declare that no competing interests exist.

  6. Huifang Lou

    Department of Neurobiology, Key Laboratory of Medical Neurobiology of Ministry of Health of China, Key Laboratory of Neurobiology, Zhejiang University School of Medicine, Hangzhou, China
    Contribution
    HL, Discussing the revision of the article, Final approval of the version to be published, Contributed unpublished essential data or reagents
    Competing interests
    The authors declare that no competing interests exist.

  7. Shumin Duan

    Department of Neurobiology, Key Laboratory of Medical Neurobiology of Ministry of Health of China, Key Laboratory of Neurobiology, Zhejiang University School of Medicine, Hangzhou, China
    Contribution
    SD, Discussing the revision of the article, Final approval of the version to be published, Conception and design
    For correspondence
    duanshumin@zju.edu.cn
    Competing interests
    The authors declare that no competing interests exist.

  8. Hao Wang

    Department of Neurobiology, Key Laboratory of Medical Neurobiology of Ministry of Health of China, Key Laboratory of Neurobiology, Zhejiang University School of Medicine, Hangzhou, China
    Contribution
    HW, Conception and design, Analysis and interpretation of data, Drafting or revising the article
    For correspondence
    haowang@zju.edu.cn
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-1862-8229

Funding

National Natural Science Foundation of China (31471022)

  • Shumin Duan
  • Hao Wang

Major State Basic Research Program of China (2011CB504400)

  • Shumin Duan
  • Hao Wang

Major State Basic Research Program of China (2013CB945600)

  • Shumin Duan
  • Hao Wang

Major State Basic Research Program of China (2015CB755600)

  • Shumin Duan
  • Hao Wang

Major State Basic Research Program of China (2015AA020515)

  • Shumin Duan
  • Hao Wang

The Program for Introducing Talents in Discipline to Universities

  • Shumin Duan

The Fundamental Research Funds for the Central Universities (2014FZA7007)

  • Shumin Duan

Zhejiang Provincial Natural Science Foundation of China (Y2110057)

  • Shumin Duan
  • Hao Wang

National Natural Science Foundation of China (81221003)

  • Shumin Duan
  • Hao Wang

National Natural Science Foundation of China (91232000)

  • Shumin Duan
  • Hao Wang

National Natural Science Foundation of China (31490590)

  • Shumin Duan
  • Hao Wang

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Acknowledgements

We thank Dr. Zhong-wei Zhang, Dr. Da-ting Lin, Dr. Xiang-yao Li, Dr. Jia-dong Chen, and Dr. IC Bruce for critical comments on this manuscript. This work was supported by grants from the Major State Basic Research Program of China (2011CB504400, 2013CB945600, 2015CB755600, 2015AA020515), the National Natural Science Foundation of China (31471022, 81221003, 91232000, 31490590), the Program for Introducing Talents in Discipline to Universities, the Zhejiang Provincial Natural Science Foundation of China (Y2110057), and the Fundamental Research Funds for the Central Universities (2014FZA7007).

Ethics

Animal experimentation: Animal experiments were conducted in accordance with the Guidelines for the Care and Use of Laboratory Animals of Zhejiang University, and were approved by Committee of Laboratory Animal Center of Zhejiang University (ZJU201501402). All surgery was performed under sodium pentobarbital anesthesia, and every effort was made to minimize suffering.

Copyright

© 2016, Yang et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 3,714
    views
  • 811
    downloads
  • 76
    citations

Views, downloads and citations are aggregated across all versions of this paper published by eLife.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Open citations (links to open the citations from this article in various online reference manager services)

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

  1. Junhua Yang
  2. Hongbin Yang
  3. Yali Liu
  4. Xia Li
  5. Liming Qin
  6. Huifang Lou
  7. Shumin Duan
  8. Hao Wang
(2016)
Astrocytes contribute to synapse elimination via type 2 inositol 1,4,5-trisphosphate receptor-dependent release of ATP
eLife 5:e15043.
https://doi.org/10.7554/eLife.15043

Share this article

https://doi.org/10.7554/eLife.15043

Categories and tags

Research organism

Further reading

    1. Neuroscience

    Associative plasticity of granule cell inputs to cerebellar Purkinje cells

    Rossella Conti, Céline Auger
    Research Article

    Granule cells of the cerebellum make up to 175,000 excitatory synapses on a single Purkinje cell, encoding the wide variety of information from the mossy fibre inputs into the cerebellar cortex. The granule cell axon is made of an ascending portion and a long parallel fibre extending at right angles, an architecture suggesting that synapses formed by the two segments of the axon could encode different information. There are controversial indications that ascending axon (AA) and parallel fibre (PF) synapse properties and modalities of plasticity are different. We tested the hypothesis that AA and PF synapses encode different information, and that the association of these distinct inputs to Purkinje cells might be relevant to the circuit and trigger plasticity, similar to the coincident activation of PF and climbing fibre inputs. Here, by recording synaptic currents in Purkinje cells from either proximal or distal granule cells (mostly AA and PF synapses, respectively), we describe a new form of associative plasticity between these two distinct granule cell inputs. We show for the first time that synchronous AA and PF repetitive train stimulation, with inhibition intact, triggers long-term potentiation (LTP) at AA synapses specifically. Furthermore, the timing of the presentation of the two inputs controls the outcome of plasticity and induction requires NMDAR and mGluR1 activation. The long length of the PFs allows us to preferentially activate the two inputs independently, and despite a lack of morphological reconstruction of the connections, these observations reinforce the suggestion that AA and PF synapses have different coding capabilities and plasticity that is associative, enabling effective association of information transmitted via granule cells.

    1. Neuroscience

    Pharyngeal neuronal mechanisms governing sour taste perception in Drosophila melanogaster

    Bhanu Shrestha, Jiun Sang ... Youngseok Lee
    Research Article

    Sour taste, which is elicited by low pH, may serve to help animals distinguish appetitive from potentially harmful food sources. In all species studied to date, the attractiveness of oral acids is contingent on concentration. Many carboxylic acids are attractive at ecologically relevant concentrations but become aversive beyond some maximal concentration. Recent work found that Drosophila ionotropic receptors IR25a and IR76b expressed by sweet-responsive gustatory receptor neurons (GRNs) in the labellum, a peripheral gustatory organ, mediate appetitive feeding behaviors toward dilute carboxylic acids. Here, we disclose the existence of pharyngeal sensors in Drosophila melanogaster that detect ingested carboxylic acids and are also involved in the appetitive responses to carboxylic acids. These pharyngeal sensors rely on IR51b, IR94a, and IR94h, together with IR25a and IR76b, to drive responses to carboxylic acids. We then demonstrate that optogenetic activation of either Ir94a+ or Ir94h+ GRNs promotes an appetitive feeding response, confirming their contributions to appetitive feeding behavior. Our discovery of internal pharyngeal sour taste receptors opens up new avenues for investigating the internal sensation of tastants in insects.

    1. Neuroscience

    Stable sequential dynamics in prefrontal cortex represents subjective estimation of time

    Yiting Li, Wenqu Yin ... Baoming Li
    Research Article

    Time estimation is an essential prerequisite underlying various cognitive functions. Previous studies identified ‘sequential firing’ and ‘activity ramps’ as the primary neuron activity patterns in the medial frontal cortex (mPFC) that could convey information regarding time. However, the relationship between these patterns and the timing behavior has not been fully understood. In this study, we utilized in vivo calcium imaging of mPFC in rats performing a timing task. We observed cells that showed selective activation at trial start, end, or during the timing interval. By aligning long-term time-lapse datasets, we discovered that sequential patterns of time coding were stable over weeks, while cells coding for trial start or end showed constant dynamism. Furthermore, with a novel behavior design that allowed the animal to determine individual trial interval, we were able to demonstrate that real-time adjustment in the sequence procession speed closely tracked the trial-to-trial interval variations. And errors in the rats’ timing behavior can be primarily attributed to the premature ending of the time sequence. Together, our data suggest that sequential activity maybe a stable neural substrate that represents time under physiological conditions. Furthermore, our results imply the existence of a unique cell type in the mPFC that participates in the time-related sequences. Future characterization of this cell type could provide important insights in the neural mechanism of timing and related cognitive functions.