1. Biophysics and Structural Biology
  2. Plant Biology
Download icon

Mechanistic insight into a peptide hormone signaling complex mediating floral organ abscission

  1. Julia Santiago
  2. Benjamin Brandt
  3. Mari Wildhagen
  4. Ulrich Hohmann
  5. Ludwig A Hothorn
  6. Melinka A Butenko
  7. Michael Hothorn Is a corresponding author
  1. University of Geneva, Switzerland
  2. University of Oslo, Norway
  3. Leibniz University, Germany
Research Article
Cited
16
Views
2,701
Comments
0
Cite as: eLife 2016;5:e15075 doi: 10.7554/eLife.15075

Abstract

Plants constantly renew during their life cycle and thus require to shed senescent and damaged organs. Floral abscission is controlled by the leucine-rich repeat receptor kinase (LRR-RK) HAESA and the peptide hormone IDA. It is unknown how expression of IDA in the abscission zone leads to HAESA activation. Here we show that IDA is sensed directly by the HAESA ectodomain. Crystal structures of HAESA in complex with IDA reveal a hormone binding pocket that accommodates an active dodecamer peptide. A central hydroxyproline residue anchors IDA to the receptor. The HAESA co-receptor SERK1, a positive regulator of the floral abscission pathway, allows for high-affinity sensing of the peptide hormone by binding to an Arg-His-Asn motif in IDA. This sequence pattern is conserved among diverse plant peptides, suggesting that plant peptide hormone receptors may share a common ligand binding mode and activation mechanism.

Article and author information

Author details

  1. Julia Santiago

    1. Structural Plant Biology Laboratory, Department of Botany and Plant Biology, University of Geneva, Geneva, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
  2. Benjamin Brandt

    1. Structural Plant Biology Laboratory, Department of Botany and Plant Biology, University of Geneva, Geneva, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
  3. Mari Wildhagen

    1. Department of Biosciences, Section for Genetic and Evolutionary Biology, University of Oslo, Oslo, Norway
    Competing interests
    The authors declare that no competing interests exist.
  4. Ulrich Hohmann

    1. Structural Plant Biology Laboratory, Department of Botany and Plant Biology, University of Geneva, Geneva, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
  5. Ludwig A Hothorn

    1. Institute of Biostatistics, Leibniz University, Hannover, Germany
    Competing interests
    The authors declare that no competing interests exist.
  6. Melinka A Butenko

    1. Department of Biosciences, Section for Genetic and Evolutionary Biology, University of Oslo, Oslo, Norway
    Competing interests
    The authors declare that no competing interests exist.
  7. Michael Hothorn

    1. Structural Plant Biology Laboratory, Department of Botany and Plant Biology, University of Geneva, Geneve, Switzerland
    For correspondence
    1. michael.hothorn@unige.ch
    Competing interests
    The authors declare that no competing interests exist.

Reviewing Editor

  1. Mingjie Zhang, Reviewing Editor, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, China

Publication history

  1. Received: February 8, 2016
  2. Accepted: April 7, 2016
  3. Accepted Manuscript published: April 8, 2016 (version 1)
  4. Version of Record published: April 27, 2016 (version 2)

Copyright

© 2016, Santiago et al

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 2,701
    Page views
  • 847
    Downloads
  • 16
    Citations

Article citation count generated by polling the highest count across the following sources: Crossref, Scopus, PubMed Central.

Comments

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Download citations (links to download the citations from this article in formats compatible with various reference manager tools)

Open citations (links to open the citations from this article in various online reference manager services)

Further reading

    1. Microbiology and Infectious Disease
    Kenneth J Ertel et al.
    Research Article