(A) P15 slice in interface chamber. Recording electrode (R) between two stimulating electrodes (S1 and S2) separated by more than 500 µm. Rectangles indicate the regions from which tissue was …
(A) Spine density (#/µm length of reconstructed dendrite) from perfusion-fixed (PF) decreased under control stimulation (Con) and increased at 120 min after TBS induction of LTP (F (2, 60)=26.45, p<0…
(A) Three neighboring presynaptic boutons located along the same axon (translucent gray). Bouton 1 (b1) was an MSB with two postsynaptic partners (PSDs, red) that contained a mitochondrion (navy), …
(A) Nondocked vesicles versus mitochondrial distance (Dm) had a statistically significant breakpoint at 2.97 ± 0.67 µm (t(165) = 4.4, p<0.001). (B) The breakpoint for docked vesicles per PSD area …
Representative axons are shown from the control (Con) and TBS sites for each time point (5’, 30’, 120’ = minutes) after the induction of LTP at P15. Each axon is oriented to illustrate sequentially …
Representative 3D reconstructions of boutons with mitochondria (navy, top row) and without mitochondria (second row) with PSDs (red), docked vesicles (blue), and nondocked vesicles (green) at (A) 5 …
Synapses with mitochondria located among the presynaptic vesicles (Mito) were compared to those without mitochondria (>1 µm to Mito). At 30 min following TBS, boutons with mitochondria had (A) fewer …
(A–B) Representative EMs of presynaptic boutons (green B) from 120 min control (A1 mito, A2 no mito) and TBS (B1 mito, B2 no mito) conditions (red arrows, PSDs in A1, B2, scale bar is 0.5 µm). (C) …
(A–B) Representative 3D reconstructions of axons from P15 and adult (Adult) control and TBS conditions at 120 min. (Total axon segment lengths – Lt, black lines – mitochondrial dimensions, length – …
(A1) The orthodox mitochondrial configuration had cristae with uniformly thin widths (<20 nm) and homogeneously gray matrices. (A2) The condensed mitochondrial configuration had wider and more …
Sample sizes for axons, boutons, vesicles, and mitochondria under each condition. Table 1 provides total n’s for each of the figures. Dashes mean that category was not used in a particular figure. Fi…
Age | P15 | Adult | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Time | Perfused | 5 min | 30 min | 120 min | 120 min | |||||
Condition | R88 | R89 | CON | LTP | CON | LTP | CON | LTP | CON | LTP |
# dendrites | 9 | 8 | 19 | 20 | 23 | 28 | 24 | 22 | 8 | 8 |
# bricks | 2 | 2 | – | – | – | – | 4 | 4 | 6, 4 | 6, 4 |
Total Brick Volume (µm2) | 54 | 65 | – | – | – | - | 147 | 125 | 165, 99 | 222, 145 |
Axons (total n) | ||||||||||
85 (44) | 77 (37) | – | - | – | – | – | – | – | – | |
– | – | 132 | 108 | 143 | 184 | 88 | 144 | – | – | |
– | – | – | – | 143 | 184 | 88 | 144 | – | - | |
– | - | – | – | – | – | – | – | 225 | 289 | |
– | – | – | – | – | – | 71 | 121 | 80 | 80 | |
Figure 9 axon length | – | – | – | – | – | – | 12.5 ± 2.9 | 13.0 ± 2.9 | 11.0 ± 1.4 | 12.6 ± 1.7 |
Boutons (total n) | ||||||||||
89 | 84 | – | – | – | – | 165 | 208 | – | – | |
77 | 113 | – | – | – | – | – | – | – | – | |
– | – | 132 | 108 | 143 | 184 | 88 | 144 | – | – | |
– | – | – | – | 143 | 184 | 88 | 144 | – | - | |
– | - | – | - | – | - | – | - | 225 | 289 | |
Mitochondria (total n) | ||||||||||
36 | 39 | – | - | – | – | – | – | – | – | |
– | – | – | - | – | - | 128 | 196 | 265 | 334 | |
– | - | – | - | – | - | 80 | 80 | 80 | 80 | |
– | – | – | – | – | – | 71 | 121 | 80 | 80 | |
– | – | – | – | – | – | 88 | 105 | 161 | 159 | |
– | – | – | – | – | – | 33 | 36 | 40 | 40 |