53BP1 and USP28 mediate p53-dependent cell cycle arrest in response to centrosome loss and prolonged mitosis

  1. Chii Shyang Fong
  2. Gregory Mazo
  3. Tuhin Das
  4. Joshua Goodman
  5. Minhee Kim
  6. Brian P O'Rourke
  7. Denisse Izquierdo
  8. Meng-Fu Bryan Tsou  Is a corresponding author
  1. Memorial Sloan Kettering Cancer Center, United States
  2. Oberlin College, United States
  3. Weill Cornell Medical School, United States

Abstract

Mitosis occurs efficiently, but when it is disturbed or delayed, p53-dependent cell death or senescence is often triggered after mitotic exit. To characterize this process, we conducted CRISPR-mediated loss-of-function screens using a cell-based assay in which mitosis is consistently disturbed by centrosome loss. We identified 53BP1 and USP28 as essential components acting upstream of p53, evoking p21-dependent cell cycle arrest in response not only to centrosome loss, but also to other distinct defects causing prolonged mitosis. Intriguingly, 53BP1 mediates p53 activation independently of its DNA repair activity, but requiring its interacting protein USP28 that can directly deubiquitinate p53 in vitro and ectopically stabilize p53 in vivo. Moreover, 53BP1 can transduce prolonged mitosis to cell cycle arrest independently of the spindle assembly checkpoint (SAC), suggesting that while SAC protects mitotic accuracy by slowing down mitosis, 53BP1 and USP28 function in parallel to select against disturbed or delayed mitosis, promoting mitotic efficiency.

Article and author information

Author details

  1. Chii Shyang Fong

    Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, United States
    Competing interests
    The authors declare that no competing interests exist.
  2. Gregory Mazo

    Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, United States
    Competing interests
    The authors declare that no competing interests exist.
  3. Tuhin Das

    Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, United States
    Competing interests
    The authors declare that no competing interests exist.
  4. Joshua Goodman

    Oberlin College, Oberlin, United States
    Competing interests
    The authors declare that no competing interests exist.
  5. Minhee Kim

    BCMB Graduate Program, Weill Cornell Medical School, New York, United States
    Competing interests
    The authors declare that no competing interests exist.
  6. Brian P O'Rourke

    Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, United States
    Competing interests
    The authors declare that no competing interests exist.
  7. Denisse Izquierdo

    BCMB Graduate Program, Weill Cornell Medical School, New York, United States
    Competing interests
    The authors declare that no competing interests exist.
  8. Meng-Fu Bryan Tsou

    Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, United States
    For correspondence
    tsoum@mskcc.org
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-2159-8836

Reviewing Editor

  1. Jon Pines, The Gurdon Institute, United Kingdom

Publication history

  1. Received: March 22, 2016
  2. Accepted: July 1, 2016
  3. Accepted Manuscript published: July 2, 2016 (version 1)
  4. Version of Record published: July 15, 2016 (version 2)

Copyright

© 2016, Fong et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Chii Shyang Fong
  2. Gregory Mazo
  3. Tuhin Das
  4. Joshua Goodman
  5. Minhee Kim
  6. Brian P O'Rourke
  7. Denisse Izquierdo
  8. Meng-Fu Bryan Tsou
(2016)
53BP1 and USP28 mediate p53-dependent cell cycle arrest in response to centrosome loss and prolonged mitosis
eLife 5:e16270.
https://doi.org/10.7554/eLife.16270

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