(A) Representative recordings of LC3 and LC5 and of network activity over one hour of TEA exposure. Roman numerals (I, II, III, IV) designate time points of reference throughout the remainder of the figure, as follows: I – control saline, II – acute TEA exposure identified as the maximum effect on loss of synchrony across LCs (i.e. lowest R2 value), III – 30 min of TEA exposure, IV – 60 min of TEA exposure. (B) Scatterplots show pairwise correlation of time-matched voltages (sampled at 10 kHz) of the waveforms shown in the representative traces. R2 values are calculated from Pearson’s correlation tests for these two cells. Loss and restoration of conserved output is demonstrated by changes in coherence in the scatterplot as well as in R2 value. (C) Synchrony of waveforms of the two cells seen in panels A and B plotted as R2 values over the entire time course of the experiment. Roman numerals and large gray circles represent the values that were obtained from the scatterplots as each time point shown in panel B. TEA perfusion persists from time zero through 60 min. Box plots show distributions of R2 values from cross-correlation analyses of LC voltage waveforms for pairs of LCs from N = 11 preparations. Lines within boxes mark the median, box boundaries represent 25th and 75th percentiles, whiskers represent 5th and 95th percentiles, and points represent outlying observations. Groups with significant differences in median synchrony (p<0.05; Wilcoxon signed rank tests) are denoted with different letters. (D) Excitability of LCs was quantified by five measurements (mean ± SD). Analysis of each preparation used the average of 10 consecutive bursts at each time point (N = 8 preparations). Number of spikes per burst, spike frequency within each burst, and the latency between pacemaker firing and first motor neuron spike (SC-LC Phase Delay) were calculated from extracellular traces. Total depolarization and amplitude of each burst are based on intracellular recordings. Significant differences across groups (p<0.05; paired t-tests) are denoted with different letters, such that any two bars with a letter in common are not significantly different.