(A) Cp110-deficient MCC cilia fail to beat directionally. gfp-cfap20 injected embryos were used to visualize ciliary beating (10 s projections are shown). (Related to Video 2–3). (B) Quantification of cilia motility data. ***p<0.001 from χ²-test. (C–D) cp110MO dose-dependent phenotypes of MCC ciliation, basal bodies and apical Actin. cp110MO doses used: 0pmol (control injected; first row), 3pmol (second row), 5pmol (third row), and 7pmol (fourth row). (C) Cilia were visualized by immunofluorescence for Acetylated-α-tubulin (cilia, Ac.-α-tub., red), rootlets were visualized by Clamp-GFP (white). n embryos/MCCs: control (6/19), cp110MO 3pmol (6/22), 5pmol (6/22), 7pmol (6/21). (D) Actin (red), basal bodies (Centrin-CFP, white), rootlets (Clamp-GFP, green). n embryos/MCCs: control (6/22), cp110MO 3pmol (6/17), 5pmol (6/22), 7pmol (6/29).