Peak intensity changes in the 600MHz 1H-15N TROSY spectra are reported for the integrin WT β1 TM/CT and two mutants (K752E, K752R) as induced either by 4 mol% 16-DSA as the hydrophobic paramagnet or by 10 mM Gd-DTPA as the water soluble paramagnet. The spectra were collected at 45°C and the samples contained ~500 µM β1 TM/CT, 20% q = 0.3 D6PC-DMPC bicelles, 1 mM EDTA, 250 mM IMD buffer at pH 6.5, with10% D2O. The overlaid spectra for WT β1 TM/CT containing no paramagnetic probe (black, bottom) and with Gd-DTPA (red, on top) are shown (A), while the corresponding spectra for the two mutants are shown in supporting Figure 3. The absence of a red peak indicates that the peak is broadened beyond detection by the presence of the paramagnetic probe. The overlay of spectra for WT β1 TM/CT containing 16-DSA (red) versus no paramagnetic probe (black) are shown (B). Panel C shows the peak intensity changes induced by 10 mM Gd-DTPA on the three forms of the integrin β1 TM/CT, while panel D shows the results for 16-DSA. The intensity ratio (I/I0) reported for each TROSY amide resonance is the observed peak intensity in the presence of the paramagnetic probe divided by the intensity of the corresponding peak under probe-free diamagnetic conditions. Two replicates of this experiment giving consistent results were performed. We show one representative example. The error is estimated from the noise level, or 5% of value, whichever is larger.