An evidence based hypothesis on the existence of two pathways of mitochondrial crista formation

  1. Max E Harner  Is a corresponding author
  2. Ann-Katrin Unger
  3. Willie JC Geerts
  4. Muriel Mari
  5. Toshiaki Izawa
  6. Maria Stenger
  7. Stefan Geimer
  8. Fulvio Reggiori
  9. Benedikt Westermann
  10. Walter Neupert  Is a corresponding author
  1. Max Planck Institute of Biochemistry, Germany
  2. Universität Bayreuth, Germany
  3. Universiteit Utrecht, Netherlands
  4. University of Groningen, Netherlands

Abstract

Metabolic function and architecture of mitochondria are intimately linked. More than 60 years ago, cristae were discovered as characteristic elements of mitochondria that harbor the protein complexes of oxidative phosphorylation, but how cristae are formed, remained an open question. Here we present experimental results obtained with yeast that support a novel hypothesis on the existence of two molecular pathways that lead to generation of lamellar and tubular cristae. Formation of lamellar cristae depends on the mitochondrial fusion machinery through a pathway that is required also for homeostasis of mitochondria and mitochondrial DNA. Tubular cristae are formed via invaginations of the inner boundary membrane by a pathway independent of the fusion machinery. Dimerization of the F1FO-ATP synthase and presence of the MICOS complex are necessary for both pathways. The proposed hypothesis is suggested to apply also to higher eukaryotes, since the key components are conserved in structure and function throughout evolution.

Article and author information

Author details

  1. Max E Harner

    Max Planck Institute of Biochemistry, Martinsried, Germany
    For correspondence
    max.harner@med.uni-muenchen.de
    Competing interests
    The authors declare that no competing interests exist.
  2. Ann-Katrin Unger

    Cell Biology and Electron Microscopy, Universität Bayreuth, Bayreuth, Germany
    Competing interests
    The authors declare that no competing interests exist.
  3. Willie JC Geerts

    Biomolecular Imaging, Bijvoet Center, Universiteit Utrecht, Utrecht, Netherlands
    Competing interests
    The authors declare that no competing interests exist.
  4. Muriel Mari

    Department of Cell Biology, University Medical Center Groningen, University of Groningen, Groningen, Netherlands
    Competing interests
    The authors declare that no competing interests exist.
  5. Toshiaki Izawa

    Max Planck Institute of Biochemistry, Martinsried, Germany
    Competing interests
    The authors declare that no competing interests exist.
  6. Maria Stenger

    Cell Biology and Electron Microscopy, Universität Bayreuth, Bayreuth, Germany
    Competing interests
    The authors declare that no competing interests exist.
  7. Stefan Geimer

    Cell Biology and Electron Microscopy, Universität Bayreuth, Bayreuth, Germany
    Competing interests
    The authors declare that no competing interests exist.
  8. Fulvio Reggiori

    Department of Cell Biology, University Medical Center Groningen, University of Groningen, Groningen, Netherlands
    Competing interests
    The authors declare that no competing interests exist.
  9. Benedikt Westermann

    Cell Biology and Electron Microscopy, Universität Bayreuth, Bayreuth, Germany
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-2991-1604
  10. Walter Neupert

    Max Planck Institute of Biochemistry, Martinsried, Germany
    For correspondence
    Neupert@biochem.mpg.de
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-0571-4419

Funding

Max-Planck-Gesellschaft

  • Max E Harner
  • Ann-Katrin Unger
  • Toshiaki Izawa
  • Walter Neupert

Carl Friedrich von Siemens Stiftung

  • Walter Neupert

Jung-Stiftung für Wissenschaft und Forschung

  • Max E Harner

Ludwig-Maximilians-Universität München

  • Max E Harner

Netherlands organization for Scientific Research (DN82-303)

  • Fulvio Reggiori

Deutsche Forschungsgemeinschaft (DN82-303)

  • Fulvio Reggiori

Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (CRSII3_154421)

  • Fulvio Reggiori

ZonMw (ZonMW VICI)

  • Fulvio Reggiori

Netherlands organization for Scientific Research (822.02.014)

  • Fulvio Reggiori

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Copyright

© 2016, Harner et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Max E Harner
  2. Ann-Katrin Unger
  3. Willie JC Geerts
  4. Muriel Mari
  5. Toshiaki Izawa
  6. Maria Stenger
  7. Stefan Geimer
  8. Fulvio Reggiori
  9. Benedikt Westermann
  10. Walter Neupert
(2016)
An evidence based hypothesis on the existence of two pathways of mitochondrial crista formation
eLife 5:e18853.
https://doi.org/10.7554/eLife.18853

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https://doi.org/10.7554/eLife.18853