The graph shows the metabolite levels and transcript expression for the Leloir pathway, glycolysis, trehalose cycle, glycerol biosynthesis, TCA cycle, and electron transport chain. Purple steps cost ATP or inorganic phosphate (Pi), while green steps generate ATP or Pi. Strains were cultured in SC + 2% galactose. The arrows are color-coded to represent the RNA-Seq gene expression differences of S. uvarum gal80∆ gal80b∆ relative to wild-type at 4 hr (red, up in the mutant; blue, down in the mutant; black, similar to wild-type; mixed colors (e.g. black and blue) indicate that the expression of genes involved in this step differs). The boxes next to each metabolite represent the log2 of the metabolite concentration differences relative to wild-type over time (0.5, 1.5, 3, 5, and 16 hr, respectively). The statistical significance for metabolite levels was assessed using Student’s t-tests (n = 3, p<0.05 with gray reported as not significant). The 1.5 hr to 5 hr time points correspond to the TGA phase, whereas the 16 hr time point corresponds to mid-log phase after recovery from the TGA phase. 1, the sum of the metabolite concentrations of glycerol-3-phosphate and glycerol-2-phosphate, the latter of which is not known to be a major metabolite in Saccharomyces; 2, the sum of the metabolite levels of 3-phosphoglycerate and 2-phosphoglycerate.